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- Title
- Unlocking the Phytotherapeutic Synergism between Beta-lapachone and Soybean-derived Genistein in Human Prostate cancer cells.
- Creator
- Oseni, Saheed Oluwasina, Sandoval-Bernal, Bibiana, Kumi-Diaka, James, Graduate College
- Abstract/Description
-
Prostate cancer after many years is still the second most common cancer in American men with about 233,000 new cases and 29,480 deaths estimated to be occurring in 2014. Despite the wide spectra of reports demonstrating the anti-cancer phytotherapeutic potentials of beta-lapachone and soybean-derived genistein in various tumors, little emphasis had been placed on their synergistic effects in androgen-independent PC3 and androgen-dependent LNCaP prostate cancer cell lines. In this study, we...
Show moreProstate cancer after many years is still the second most common cancer in American men with about 233,000 new cases and 29,480 deaths estimated to be occurring in 2014. Despite the wide spectra of reports demonstrating the anti-cancer phytotherapeutic potentials of beta-lapachone and soybean-derived genistein in various tumors, little emphasis had been placed on their synergistic effects in androgen-independent PC3 and androgen-dependent LNCaP prostate cancer cell lines. In this study, we aim to characterize the combined effects of genistein and b-lapachone on the phyto/chemosensitivity of LNCaP and PC3 human prostate cancer cells in-vitro, using MTT assay and LDH assay to study treatment-induced growth inhibition and cytotoxicity. Annexin-V-FITC and PI-TUNEL assays were also used to determine the potential treatment-induced apoptosis and/or necrosis. Our results revealed that both PC3 and LNCaP are phytosensitive to both single and combined treatments, though time-and dose-dependent. We observed that our treatments induced dual death pathways-apoptosis and necrosis-in both cell types and also observed that growth inhibition in both correlated positively with cell death in which, b-lapachone and genistein induced cell cycle arrest at the G1 and/or S phase and G2–M checkpoints respectively. Invariably, our results indicate that combination treatments with b-lapachone and genistein are more potent in killing both PC3 and LNCaP cancer cells than treatment with either genistein or b-lapachone alone. Our current results are therefore in agreement with the hypothesis that drugcombinations that target cell cycles at different critical checkpoints are more effective in causing cell death.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00005845
- Format
- Document (PDF)
- Title
- Pyroelectric Crystal Generated Very Low Dose X-rays Enhanced the Phytotherapeutic Effects of Beta-lapachone in Hormone Dependent Prostate Cancer Cells In Vitro.
- Creator
- Oseni, Saheed Oluwasina, Kumi-Diaka, James, Jebelli, Joubin, Graduate College, Goldsmith, Harris, Kaldas, George, Branly, Rolando
- Abstract/Description
-
In 2015, an estimated 220, 800 new cases and 27, 540 deaths are expected to occur due to prostate cancer in US men, thus adding to the economic burden of the over 2.6 million men currently battling the disease. Plethora of studies have demonstrated the phytotherapeutic potentials of beta-lapachone, a phytochemical compound derived from the bark of the lapacho tree, native to South America. Betalapachone (β-lap) has been shown to exhibit its anti-cancer effects majorly by the futile cycling...
Show moreIn 2015, an estimated 220, 800 new cases and 27, 540 deaths are expected to occur due to prostate cancer in US men, thus adding to the economic burden of the over 2.6 million men currently battling the disease. Plethora of studies have demonstrated the phytotherapeutic potentials of beta-lapachone, a phytochemical compound derived from the bark of the lapacho tree, native to South America. Betalapachone (β-lap) has been shown to exhibit its anti-cancer effects majorly by the futile cycling between the oxidized and the two electron reduction of β-lap mediated by NAD(P)H:quinone oxidoreductase (NQO1) resulting in the generation of reactive oxygen species (ROS) using NADH or NAD(P) as electron sources. β-lap is known to selectively kill human cancer cells, since NQO1 is expressed more abundantly in numerous human solid tumors than in the adjacent normal tissues; NQO1 has been shown to be exceptionally under expressed in hormone dependent prostate cancer cells (LNCaP) compared to the hormone independent prostate cancer cells (PC3). This study was aimed to investigate the enhancing effects of very low dose radiation (VLDR (20mGy)) derived from a pyroelectric crystal generator on the phytotherapeutic activity of beta-lapachone in LNCaP cell line in vitro accessed by MTT and Trypan blue assay. Treatment-induced intracellular levels of ROS were also assessed using Nitro blue tetrazolium assay. NQO1 activities in LNCaP cells were also investigated following treatment with VLDR and/or β-lap using Dicoumarol (NQO1 inhibitor). Results indicate that LNCaP cells respond significantly to combined treatments compared to single treatments.
Show less - Date Issued
- 2015
- PURL
- http://purl.flvc.org/fau/fd/FA00005905
- Format
- Document (PDF)
- Title
- Impact of Vitamin C on Genistein induced apoptosis in treatment of prostate cancer cells.
- Creator
- Famuyiwa, Toluleke, Boe, Andrew, Esiobu, Nwadiuto, Graduate College, Kumi-Diaka, James
- Abstract/Description
-
Background: Prostate Cancer, in the absence of skin cancer, is the most prevalent type of cancer found in the male population. Reactive Oxygen Species (ROS) can promote cancer cell proliferation when they are at elevated levels. Vitamin C is a water-soluble antioxidant capable of inhibiting the formation of ROS. Genistein, an isoflavone found in plants, also possesses the ability to inhibit ROS formation. Objective To determine the potential therapeutic synergy between genistein and vitamin C...
Show moreBackground: Prostate Cancer, in the absence of skin cancer, is the most prevalent type of cancer found in the male population. Reactive Oxygen Species (ROS) can promote cancer cell proliferation when they are at elevated levels. Vitamin C is a water-soluble antioxidant capable of inhibiting the formation of ROS. Genistein, an isoflavone found in plants, also possesses the ability to inhibit ROS formation. Objective To determine the potential therapeutic synergy between genistein and vitamin C and investigate mechanism of action of genistein and/or vitamin C. Methods: Trypan blue assay was carried out to know the % of viable cells. Varying concentrations of genistein with a constant concentration of Vitamin C was used to treat LNCaP cells. After treatment of the cells with genistein and Vitamin C, MTT assay of the cancer cells was performed and absorbance read through an ELISA reader. This gives the values needed for interpreting cell viability after treatment. A statistical analysis performed to determine whether the obtained results are statistically significant. Results: The results obtained from our experiments are inconclusive with regards to the impact of Vitamin C on apoptotic cancer cell death following genistein treatment. However the combination of genistein and vitamin C was more efficient in tumor suppression than when the drugs were given separately. Conclusion: This study suggests that treatment of prostate cancer using genistein can be enhanced by adjuvant treatment with vitamin C. This study is of potential clinical success in reducing the cell death by necrosis.
Show less - Date Issued
- 2015
- PURL
- http://purl.flvc.org/fau/fd/FA00005876
- Format
- Document (PDF)
- Title
- Anticancer activities of genistein-topotecan combination in LNCaP prostate cancer cells.
- Creator
- Hörmann, Vanessa P., Kumi-Diaka, James, Graduate College
- Date Issued
- 2011-04-08
- PURL
- http://purl.flvc.org/fcla/dt/3164543
- Subject Headings
- Prostate --Cancer --Alternative treatment, Cancer --Chemotherapy, Isoflavones --therapeutic use
- Format
- Document (PDF)
