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Effects of GUS Gene Integration in Tobacco Plants
Title
Effects of GUS Gene Integration in Tobacco Plants.
Creator
Cruz, Laura, Zhang, Xing-Hai
Abstract/Description
The β-glucuronidase (GUS) gene was isolated in 1986 from the bacterium Escherichia coli. Since then it has been widely used as a reporter gene in genetically modified organisms serving to study gene expression and tissue specificity of different promoter sequences. We have introduced the GUS gene into tobacco plants through Agrobacterium-mediated genomic transformation. The plants that were confirmed to be expressing the GUS gene were grown to propagate a new (T1) generation. The T1 plants...
Show moreThe β-glucuronidase (GUS) gene was isolated in 1986 from the bacterium Escherichia coli. Since then it has been widely used as a reporter gene in genetically modified organisms serving to study gene expression and tissue specificity of different promoter sequences. We have introduced the GUS gene into tobacco plants through Agrobacterium-mediated genomic transformation. The plants that were confirmed to be expressing the GUS gene were grown to propagate a new (T1) generation. The T1 plants were analyzed for tissue specificity of GUS expression. The results to date seem to indicate that there is some variation in GUS expression between plant lines. The mechanisms of GUS gene integration in the plant genome as well as the possible effects it can have on a plant’s genomic structure are assessed.
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Date Issued
2014
PURL
http://purl.flvc.org/fau/fd/FA0005009
Subject Headings
College students --Research --United States.
Format
Document (PDF)
Genetic Engineering of Tomato Plants Expressing β-Glucuronidasethrough Agrobacterium-mediated Transformation
Title
Genetic Engineering of Tomato Plants Expressing β-Glucuronidasethrough Agrobacterium-mediated Transformation.
Creator
Justs, Adriana, Kaplan, Noah, Zhang, Xing-Hai, Office of Undergraduate Research and Inquiry
Abstract/Description
Advancements in biotechnology have allowed us to study genetics and plant physiology by engineering transgenic plants. For our research we transformed Micro-Tom, a tomato variety developed for use in genetic research, using Agrobacterium mediated transformation. Within a time span of fourteen weeks, we inserted two distinct plasmid constructs (pCAMBIA2301 and E1492). Plants have the unique ability to regenerate their tissue and we took advantage of this ability to regenerate the transgenic...
Show moreAdvancements in biotechnology have allowed us to study genetics and plant physiology by engineering transgenic plants. For our research we transformed Micro-Tom, a tomato variety developed for use in genetic research, using Agrobacterium mediated transformation. Within a time span of fourteen weeks, we inserted two distinct plasmid constructs (pCAMBIA2301 and E1492). Plants have the unique ability to regenerate their tissue and we took advantage of this ability to regenerate the transgenic plants with antibiotic selection. Approximately one third of the explants endured the infection process and fourteen of these survived in the presence of kanamycin. By the end of the fourteenth week, eleven out of our fourteen plantlets had fully developed roots but only four survived to maturity. After verification with PCR and qPCR, we found that we generated two transgenic plants. Here we describe all the methods and techniques used to achieve these compelling results.could be the potential cause of this neurodegenerative disease, will help elucidate the role of this amino acid in ALS.
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Date Issued
2016
PURL
http://purl.flvc.org/fau/fd/FA00005578
Subject Headings
College students --Research --United States.
Format
Document (PDF)
Diagnosis of Citrus Greening Disease by qPCR Analysis
Title
Diagnosis of Citrus Greening Disease by qPCR Analysis.
Creator
Rocha, Fernando, Zhang, Xing-Hai
Abstract/Description
Citrus greening, also known as Huanglongbing disease, is a phloem restrictive disease that affects orange as well as other citrus trees. The disease is caused by the gram negative bacteria Candidatus Liberibacter asiaticus. The bacteria is transmitted by the Asian psyllid, Diaphorina citri. The bacteria causes the tree to produce small and bitter oranges, the roots shrink and the leaves molt. There is currently no cure for this disease. The best way to manage citrus greening is by removing...
Show moreCitrus greening, also known as Huanglongbing disease, is a phloem restrictive disease that affects orange as well as other citrus trees. The disease is caused by the gram negative bacteria Candidatus Liberibacter asiaticus. The bacteria is transmitted by the Asian psyllid, Diaphorina citri. The bacteria causes the tree to produce small and bitter oranges, the roots shrink and the leaves molt. There is currently no cure for this disease. The best way to manage citrus greening is by removing infected trees, implementing healthy planting material and controlling the psyllid population. Quantitative real time PCR (qPCR) was used to verify whether or not a given orange tree had citrus greening disease. DNA was extracted from leaves from eight trees. A qPCR analysis was performed using a primer with the bacteria DNA. Three trees were successfully diagnosed with citrus greening using this method.
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Date Issued
2018
PURL
http://purl.flvc.org/fau/fd/FAU_SR00000051
Subject Headings
College students --Research --United States.
Format
Document (PDF)
Regulatory Pattern of PUN Promoter for Gene Expression
Title
Regulatory Pattern of PUN Promoter for Gene Expression.
Creator
Velez, Stephanie, Kirke, Justin, Zhang, Xing-Hai, Office of Undergraduate Research and Inquiry
Abstract/Description
The purpose of this research was to analyze the regulatory pattern of the PUN promoter in the expression of a marker gene, β-glucoronidase (GUS), within regenerated tobacco plants. The genes for neomycin phosphotransferase (NPT II) and GUS were included in the coding region of the Ti plasmid construct. The NPTII gene drove antibiotic resistance and was used to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical staining and genetic...
Show moreThe purpose of this research was to analyze the regulatory pattern of the PUN promoter in the expression of a marker gene, β-glucoronidase (GUS), within regenerated tobacco plants. The genes for neomycin phosphotransferase (NPT II) and GUS were included in the coding region of the Ti plasmid construct. The NPTII gene drove antibiotic resistance and was used to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical staining and genetic assays rendered putative transgenic lines that were cultivated for further assessment of progeny. First generation histochemical analysis of 14-day tissue formation resulted in no levels of expression for the GUS gene, which demonstrated that the flower-specific PUN promoter was not active in the leaf tissue. Further testing of gene activity throughout all stages of tissue formation for the first generation lines is required in order to assess regulatory pattern of the PUN promoter.
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Date Issued
2016
PURL
http://purl.flvc.org/fau/fd/FA00005606
Subject Headings
College students --Research --United States.
Format
Document (PDF)