Current Search: Reoviruses (x)
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- Title
- Differential cell killing of normal and transformed human lung fibroblasts by reovirus.
- Creator
- Loeffler, Jennifer L., Florida Atlantic University, Roner, Michael R.
- Abstract/Description
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When infected with reovirus ST3, strain Dearing, normal and transformed human lung fibroblasts exhibit differential sensitivities. Transformed cells (WI38 VA13 2RA) are destroyed within four days. In contrast, normal cells (WI38) maintain a productive infection for as long as two months. Attempts to examine the differences between these cells included the use of cDNA subtraction and a reovirus sigma 1 protein affinity chromatography column, both of which were hampered by technical...
Show moreWhen infected with reovirus ST3, strain Dearing, normal and transformed human lung fibroblasts exhibit differential sensitivities. Transformed cells (WI38 VA13 2RA) are destroyed within four days. In contrast, normal cells (WI38) maintain a productive infection for as long as two months. Attempts to examine the differences between these cells included the use of cDNA subtraction and a reovirus sigma 1 protein affinity chromatography column, both of which were hampered by technical difficulties. Two-dimensional gel electrophoresis revealed patterns of differential protein expression between infected and mock-infected normal and transformed cells. Visual comparison of Coomassie blue-stained gels revealed one protein which was present in uninfected normal cells but was missing in all other samples, as well as five proteins that were present in infected and mock-infected normal cells but were missing in both transformed cell samples. Autoradiography of 35S-labeled cell samples revealed an additional eleven proteins not seen with Coomassie staining. Further characterization of these proteins should uncover the mechanism of differential cell killing by reovirus.
Show less - Date Issued
- 2000
- PURL
- http://purl.flvc.org/fcla/dt/15776
- Subject Headings
- Reoviruses, Fibroblasts, Cell transformation
- Format
- Document (PDF)
- Title
- Identification and characterization of a newly discovered reovirus strain.
- Creator
- Jerusalmi, Alan, Florida Atlantic University, Roner, Michael R., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
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In this study, we report the identification and characterization of a new Reovirus strain isolated from the spinal fluid of a human patient. Unlike other reovirus serotypes that can be isolated from fecal matter, this strain was isolated from the spinal fluid of a patient; which suggests it may be a more pathogenic strain. The new isolate was compared to the three known strains of mammalian reoviruses. We have found that the virus is not a reassortment of the other three serotypes. This was...
Show moreIn this study, we report the identification and characterization of a new Reovirus strain isolated from the spinal fluid of a human patient. Unlike other reovirus serotypes that can be isolated from fecal matter, this strain was isolated from the spinal fluid of a patient; which suggests it may be a more pathogenic strain. The new isolate was compared to the three known strains of mammalian reoviruses. We have found that the virus is not a reassortment of the other three serotypes. This was confirmed by examination of the migration rates of the genomic dsRNA segments of all four viruses. The new isolate demonstrated measurable differences in viral protein molecular weights, when compared to the proteins of the three known serotypes. The molecular weight of the sigma I protein of the new isolate, was found to be more then 3Kd smaller then sigma 1 obtained from the other three serotypes. The largest difference in protein molecular weights was found with mu1C, which was about 7Kd smaller. The growth rate of the new virus was very similar to that of the other three serotypes. Some differences were found in select cell lines.
Show less - Date Issued
- 2000
- PURL
- http://purl.flvc.org/fcla/dt/12684
- Subject Headings
- Reoviruses, RNA viruses
- Format
- Document (PDF)
- Title
- Resistant or persistent reovirus infections: Of normal human embryonic lung fibroblast WI-38 cells.
- Creator
- Eukitis, Martine Marie., Florida Atlantic University, Roner, Michael R.
- Abstract/Description
-
Reovirus is a common virus that usually affects children; this infection causes symptoms such as respiratory and or gastrointestinal aliments. Morbidity most often occurs in impoverished countries where supportive hospitalization is not available. In the U.S. and other established countries morbidity is not an issue. When WI-38 cells are infected with reovirus the infection is either resisted by the cells or a persistent latent infection occurs. In this study, gene expression was analyzed by...
Show moreReovirus is a common virus that usually affects children; this infection causes symptoms such as respiratory and or gastrointestinal aliments. Morbidity most often occurs in impoverished countries where supportive hospitalization is not available. In the U.S. and other established countries morbidity is not an issue. When WI-38 cells are infected with reovirus the infection is either resisted by the cells or a persistent latent infection occurs. In this study, gene expression was analyzed by comparing Reovirus-infected WI-38 cells with mock infected cells. P.R.O.M(TM) analysis was performed on RNA sent to Genka Biotechnology Inc. Bioinformatics was used to analyze the data. Reovirus infection of the WI-38 cells resulted in increased mRNA levels for a number of transcription regulatory genes, and possibly more significant, decreased mRNA levels for some very important regulatory genes. These changes may be responsible for establishing the antiviral replication environment observed in these normal cells.
Show less - Date Issued
- 2003
- PURL
- http://purl.flvc.org/fcla/dt/12991
- Subject Headings
- Reoviruses, Genetic regulation, Fibroblasts
- Format
- Document (PDF)
- Title
- Selective destruction of transformed cells by reovirus: Cellular targets and active reovirus proteins analysis.
- Creator
- Alter, Sarah., Florida Atlantic University, Roner, Michael R.
- Abstract/Description
-
It is known that Reovirus selectively destroys transformed cells, but the complex mechanism is not completely understood at this time. In this study, gene expression was examined by comparing Reovirus-infected SV-40 transformed human embryonic fibroblasts, with mock-infected cells. Among the 40 genes shown to be altered, 38 genes were up regulated, and 2 genes were down regulated. Out of the 40 genes having differential gene expression, 8 were significantly over induced. 3 of these were DNA...
Show moreIt is known that Reovirus selectively destroys transformed cells, but the complex mechanism is not completely understood at this time. In this study, gene expression was examined by comparing Reovirus-infected SV-40 transformed human embryonic fibroblasts, with mock-infected cells. Among the 40 genes shown to be altered, 38 genes were up regulated, and 2 genes were down regulated. Out of the 40 genes having differential gene expression, 8 were significantly over induced. 3 of these were DNA binding transcription factors. Activation of transcription factors following Reovirus infection suggests that gene expression is essential in Reovirus induced transformed cell killing. Another 3 genes were found to be tumor suppressor proteins and oncogenes expressed downstream of the Ras pathway. The over expression of these was shown to induce apoptosis induced cell killing by Reovirus. Finally, 2 of the 8 significantly up regulated genes cause cell cycle progression inhibition. The cell cycle arrest then leads to cell death.
Show less - Date Issued
- 2002
- PURL
- http://purl.flvc.org/fcla/dt/12966
- Subject Headings
- Reoviruses, Gene expression
- Format
- Document (PDF)
- Title
- An investigation of the nonstructural proteins of mammalian reovirus serotype 3: Mu NS and sigma NS.
- Creator
- Van Vliet, Kim Marie, Florida Atlantic University, Roner, Michael R.
- Abstract/Description
-
The reovirus nonstructural proteins muNS and sigmaNS are thought to play a role in assortment. To produce large quantities of the reovirus nonstructural proteins, two recombinant vaccinia virus systems were used, one for expression of muNS and the other for expression of muNS. The reovirus gene is under the control of the phage T7 RNA polymerase promoter. A recombinant vaccinia virus containing the gene for T7 RNA polymerase was added and protein expression was determined. Protein expression...
Show moreThe reovirus nonstructural proteins muNS and sigmaNS are thought to play a role in assortment. To produce large quantities of the reovirus nonstructural proteins, two recombinant vaccinia virus systems were used, one for expression of muNS and the other for expression of muNS. The reovirus gene is under the control of the phage T7 RNA polymerase promoter. A recombinant vaccinia virus containing the gene for T7 RNA polymerase was added and protein expression was determined. Protein expression was confirmed by infecting mouse fibroblast L929 cells, harvesting the cells and running an SDS-PAGE gel, followed by western blot, followed by Western-immunoperoxidase assay using a polyclonal antibody for the reovirus proteins, or by radioimmunoprecipitation followed by an SDS-PAGE gel. Purification of the recombinant proteins was accomplished by ammonium sulfate fractionation and column chromatography. The purified proteins will be utilized to further investigate the role of the reovirus nonstructural proteins in assortment.
Show less - Date Issued
- 2002
- PURL
- http://purl.flvc.org/fcla/dt/12930
- Subject Headings
- Reoviruses, RNA viruses
- Format
- Document (PDF)