Current Search: Photosynthetic pigments--Analysis (x)
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- Title
- Photosynthetic pigment ratios in relation to Photic Flux.
- Creator
- Grant, Cidya S., Louda, J. William, Florida Atlantic University
- Abstract/Description
-
The molar and weight ratios of the photosynthetic pigments of ten algal species were analyzed in an attempt to determine if knowledge of the light field could be used to give better ratios for estimating the chlorophyll a contributed from each algal division to total chlorophyll a or biomass. High performance liquid chromatography (HPLC) is one of the tools used in pigment-based chemotaxonomy for estimating the biomass of algal communities by determining the concentrations of the various...
Show moreThe molar and weight ratios of the photosynthetic pigments of ten algal species were analyzed in an attempt to determine if knowledge of the light field could be used to give better ratios for estimating the chlorophyll a contributed from each algal division to total chlorophyll a or biomass. High performance liquid chromatography (HPLC) is one of the tools used in pigment-based chemotaxonomy for estimating the biomass of algal communities by determining the concentrations of the various chlorophylls and carotenoids. Stable ratios of chlorophyll a: marker pigment are used as multiplying factors for calculating the amount of chlorophyll a contributed from each major algal group to total chlorophyll a. Stable chlorophyll a : marker pigment ratios are not always easy to determine, so light field was investigated as a factor for determining consistently reliable ratios. This data will serve to determine new chlorophyll a: marker pigment ratios, as well as to verify or suggest changes to the existing ratios that are currently being used by our research group. An unknown pigment was isolated from Scytonema sp. grown in higher light fields and the ecological significance of this pigment was assessed.
Show less - Date Issued
- 2006
- PURL
- http://purl.flvc.org/fau/fd/FA00000768
- Subject Headings
- Photosynthetic pigments--Analysis, Marine algae, Plant pigments--Analysis, High performance liquid chromatography
- Format
- Document (PDF)
- Title
- Pigment analysis of benthic and pelagic algae in Lake Okeechobee, Florida.
- Creator
- Winfree, Nancy, Florida Atlantic University, Baker, Earl W.
- Abstract/Description
-
Water samples from Lake Okeechobee, Florida have been analyzed in order to relate pigment fingerprints to temporal and spatial variations. Chemical taxonomic identification of predominant photoautotrophs was based on pigment profiles evolved from literature reports and analysis of specific cultures. Carotenoids and chlorophylls were analyzed using high performance liquid chromatography (HPLC) and ultra-violet visible spectra obtained with a photo diode array detector (PDA). Pigments were...
Show moreWater samples from Lake Okeechobee, Florida have been analyzed in order to relate pigment fingerprints to temporal and spatial variations. Chemical taxonomic identification of predominant photoautotrophs was based on pigment profiles evolved from literature reports and analysis of specific cultures. Carotenoids and chlorophylls were analyzed using high performance liquid chromatography (HPLC) and ultra-violet visible spectra obtained with a photo diode array detector (PDA). Pigments were identified by coincident matches of retention time and UV/Visible spectral properties. Chemical taxonomic identification was determined by comparison with known photoautotrophic pigment 'fingerprints'. Experimental results show pigment arrays indicative of chromophytes, chlorophytes and cyanophytes as well as photoautotrophic bacteria present in Lake Okeechobee. The lake was also found to be heterogeneous and dynamic in terms of pigment composition. Major pigment groups changed as a function of location in the lake and as a function of season. A greater pigment diversity was found to exist in the benthos due to the presence of degradation products. HPLC-PDA pigment analysis for chemotaxonomic typing of photoautotrophic communities is attainable and can be used as a rapid viable alternative to microscopic examination of plankton and epiphyte samples. Also, taxonomic typing of organic matter and photoautotrophic communities of sediments, including bacterial populations, can be accomplished using this method.
Show less - Date Issued
- 1996
- PURL
- http://purl.flvc.org/fcla/dt/15261
- Subject Headings
- Plant pigments--Analysis, Photosynthetic pigments--Analysis, Freshwater algae, High performance liquid chromatography, Okeechobee, Lake (Fla)
- Format
- Document (PDF)