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The discovery of marine natural products with therapeutic potential
Title
The discovery of marine natural products with therapeutic potential.
Creator
McConnell, O. J., Longley, Ross E., Koehn, F. E., Harbor Branch Oceanographic Institute
Date Issued
1994
PURL
http://purl.flvc.org/fau/fd/FA00007447
Subject Headings
Marine natural products, Drug Discovery, Pharmacognosy
Format
Document (PDF)
Development of sponge cell cultures for biomedical application
Title
Development of sponge cell cultures for biomedical application.
Creator
Pomponi, Shirley A., Willoughby, Robin
Date Issued
2000
PURL
http://purl.flvc.org/FCLA/DT/3172822
Subject Headings
Natural products, Pharmacognosy, Bioactive compounds, Sponges, Sponges --Cytology
Format
Document (PDF)
Discorhabdins S, T, and U, New Cytotoxic Pyrroloiminoquinones from a Deep‐Water Caribbean Sponge of the Genus Batzella
Title
Discorhabdins S, T, and U, New Cytotoxic Pyrroloiminoquinones from a Deep‐Water Caribbean Sponge of the Genus Batzella.
Creator
Gunasekera, Sarath P., Zuleta, Ignacio A., Longley, Ross E, Wright, Amy E., Pomponi, Shirley A.
Date Issued
2003
PURL
http://purl.flvc.org/FCLA/DT/3164103
Subject Headings
Sponges, Nuclear magnetic resonance spectroscopy, Antineoplastic agents, Mass spectrometry, Pharmacognosy
Format
Document (PDF)
Terpene Biosynthesis in the Octocorals Erythropodium caribaeorum and Plexaurella spp.
Title
Terpene Biosynthesis in the Octocorals Erythropodium caribaeorum and Plexaurella spp.
Creator
Frenz, Jamie L., Kerr, Russell G., Florida Atlantic University
Abstract/Description
The marine environment is a prolific source of novel compounds for therapeutic use due to the complex biological and chemical diversity. Throughout the past 30-40 years, over 15,000 natural products have been discovered from the oceans, many of which display a broad range of potential clinical and commercial applications. Many marine invertebrates are sessile organisms that lack physical protection, and which chemical defense may be a possible explanation for these secondary metabolites....
Show moreThe marine environment is a prolific source of novel compounds for therapeutic use due to the complex biological and chemical diversity. Throughout the past 30-40 years, over 15,000 natural products have been discovered from the oceans, many of which display a broad range of potential clinical and commercial applications. Many marine invertebrates are sessile organisms that lack physical protection, and which chemical defense may be a possible explanation for these secondary metabolites. Despite the promise marine natural products have as potent pharmaceutical agents, one of the major factors delaying clinical use is the supply issue. These bioactive compounds are often found in trace amounts in the host organism, which makes harvesting from the reefs unfeasible. A general goal in our lab was to investigate the biosynthesis of secondary metabolite terpenes to ultimately provide a production method of these potent marine derived compounds. Eleutherobin and desmethyleleutherobin are diterpenes isolated from the Caribbean soft coral Erythropodium caribaeorum. These extremely valuable anticancer agents disrupt cell division by polymerizing and stabilizing microtubules, and have demonstrated tumor tissue selectivity toward selected breast, renal, ovarian and lung cancer cell lines. Determining the first intermediate in terpene biosynthesis is the initial step in developing a biotechnological production method of these cytotoxic agents. We investigated the complex chemistry of this coral using a radioactivity-guided isolation procedure, and isolated and partially characterized a diterpene hydrocarbon from E. caribaeorum. The close association between marine invertebrates, zooxanthellae and numerous bacteria gives rise to the question of the identity of the producer of secondary metabolites in marine organisms. If the symbiont produces these therapeutic agents, cell culture methods could be employed to supply the compounds rather than obtaining them from coral reefs. Sesquiterpenes have been isolated from the gorgonian Plexaurella spp., however, no investigations concerning host/symbiont contribution of the sesquiterpenes have been reported. We investigated the biosynthetic source of terpenes in this coral, and experimental evidence indicates that bacteria are responsible for sesquiterpene production. We also examined sesquiterpene variation of Plexaurella spp. from various locations, and found sesquiterpene content to vary within and between species, identifying Plexaurella as a chemically indistinguishable genus.
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Date Issued
2006
PURL
http://purl.flvc.org/fau/fd/FA00000853
Subject Headings
Coral reef ecology, Terpenes--Synthesis, Marine pharmacology, Pharmacognosy
Format
Document (PDF)
Elucidation of pseudopterosin biosynthesis: Oxidations of erogorgiaene and the intermediacy of seco-pseudopterosins
Title
Elucidation of pseudopterosin biosynthesis: Oxidations of erogorgiaene and the intermediacy of seco-pseudopterosins.
Creator
Ferns, Tyrone A., Florida Atlantic University, Kerr, Russell G.
Abstract/Description
The pseudopterosins and seco-pseudopterosins are diterpene glycosides isolated from the marine octocoral Pseudopterogorgia elisabethae . These compounds exhibit potent anti-inflammatory and analgesic properties superior to the industrial standard indomethacin. The overall goal of this work was to complete biosynthetic studies for the development of a biotechnological production method of the pseudopterosins and seco-pseudopterosins. The aim of this project was to identify all intermediates...
Show moreThe pseudopterosins and seco-pseudopterosins are diterpene glycosides isolated from the marine octocoral Pseudopterogorgia elisabethae . These compounds exhibit potent anti-inflammatory and analgesic properties superior to the industrial standard indomethacin. The overall goal of this work was to complete biosynthetic studies for the development of a biotechnological production method of the pseudopterosins and seco-pseudopterosins. The aim of this project was to identify all intermediates involved in pseudopterosin biosynthesis. Previously, the Kerr lab has shown that elisabetatriene and erogorgiaene are the first two committed steps in pseudopterosin biosynthesis. In a continuation of those metabolic studies, the isolation and utilization of 7-hydroxyerogorgiaene, 7,8-dihydroxyerogorgiaene, seco-pseudopterosin J, amphilectosins A and B in pseudopterosin biosynthesis is reported. The utilization of these compounds in pseudopterosin biosynthesis was assessed by incubating these compounds in radiolabeled form with viable cell-free extracts of P. elisabethae and monitoring for the incorporation of a radiolabel in the pseudopterosins. We also report the isolation of the pseudopterosin aglycone from extracts of P. elisabethae and identified the aglycone as the end product of this metabolic pathway.
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Date Issued
2005
PURL
http://purl.flvc.org/fcla/dt/12164
Subject Headings
Marine pharmacology, Aquaculture, Biological products, Natural products, Pharmacognosy
Format
Document (PDF)
Design considerations in high-throughput automation for biotechnology protocols
Title
Design considerations in high-throughput automation for biotechnology protocols.
Creator
Cardona, Aura, Roth, Zvi S., Florida Atlantic University, College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science
Abstract/Description
In this dissertation a computer-aided automation design methodology for biotechnology applications is proposed that leads to several design guidelines. Because of the biological nature of the samples that propagate in the automation line, a very specific set of environmental and maximum allowed shelf time conditions have to be followed to obtain good yield. In addition all biotechnology protocols require precise sequence of steps, the samples are scarce and the reagents are costly, so no...
Show moreIn this dissertation a computer-aided automation design methodology for biotechnology applications is proposed that leads to several design guidelines. Because of the biological nature of the samples that propagate in the automation line, a very specific set of environmental and maximum allowed shelf time conditions have to be followed to obtain good yield. In addition all biotechnology protocols require precise sequence of steps, the samples are scarce and the reagents are costly, so no waste can be afforded.
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Date Issued
2014
PURL
http://purl.flvc.org/fau/fd/FA00004272, http://purl.flvc.org/fau/fd/FA00004272
Subject Headings
Biotechnological process control, Biotechnological process monitoring, Molecular biology -- Automation, Molecular biology -- Technique, Molecular cloning -- Technique, Pharmacognosy
Format
Document (PDF)
Isolation and Structural Elucidation of Novel Bioactive Natural Products from Marine Organisms of the Western Atlantic Ocean
Title
Isolation and Structural Elucidation of Novel Bioactive Natural Products from Marine Organisms of the Western Atlantic Ocean.
Creator
Zhang, Long, West, Lyndon, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
Abstract/Description
The aim of this dissertation was to elaborate the exploration of biologically active secondary metabolites from the marine sponge Cacospongia cf. linteiformis collected from the Bahamas and the soft coral Briareum asbestinum collected from two different sites in Florida State, Boca Raton and Dry Tortugas. In chapter one, a review on previous chemical and biological studies of the marine sponge C. cf. linteiformis and soft coral B. asbestinum is provided. Particular attention is given to...
Show moreThe aim of this dissertation was to elaborate the exploration of biologically active secondary metabolites from the marine sponge Cacospongia cf. linteiformis collected from the Bahamas and the soft coral Briareum asbestinum collected from two different sites in Florida State, Boca Raton and Dry Tortugas. In chapter one, a review on previous chemical and biological studies of the marine sponge C. cf. linteiformis and soft coral B. asbestinum is provided. Particular attention is given to spongianolides and briarellins, two important classes of compounds isolated from C. cf. linteiformis and B. asbestinum, respectively, and their structural features and diverse bioactivities. In chapter two, the isolation and relative configuration determination of four epimeric sesterterpenoids, spongianolides E & F (18c, 18d, 19c, 19d) from C. cf. linteiformis collected from the Bahamas are discussed. Thanks to chemical modification (acetylation), diastereomeric 18c&18d and 19c&19d, respectively, were able to be isolated using chromatographic techniques for the first time, and then the relative configurations of 18c, 18d, 19c, 19d were determined based on NOESY NMR experiments. The bioactivity of mixture of compounds 18c, 18d, 19c, 19d were tested and it exhibited inhibition against Schnurri-3 (a regulator of postnatal bone mass). In chapter three, the isolation and structural elucidation of four new compounds, florellins A-D (49-52), from B. asbestinum collected off the coast of Boca Raton, FL are discussed. The molecular structures of these compounds were established by spectroscopic analysis. Compounds 49-52 are the first briarellins containing an acyl group at C-13, while 49 and 50 are the first briarellins possessing acylation at C-15. Florellins A–C (49-51) were screened and found cytotoxic against three human cell lines, BT474, WM266−4 and HEK293. In chapter four, the isolation and structural elucidation of four new compounds, florellins E-H (57-60), from B. asbestinum collected in Dry Tortugas, FL are discussed. The molecular structures of these compounds were established by spectroscopic analysis. Florellins F (58) and H (60) were screened against three human cell lines, BT474, WM266−4 and HEK293, but no cytotoxicity was exhibited. In chapter five, all the experimental procedures are described, including analytical instruments, animal materials, extraction and isolation processes, spectroscopic data and protocols of bioassays.
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Date Issued
2017
PURL
http://purl.flvc.org/fau/fd/FA00004850, http://purl.flvc.org/fau/fd/FA00004850
Subject Headings
Pharmacognosy., Natural products--Analysis., Marine pharmacology., Marine biotechnology., Marine algae--Biotechnology., Bioactive compounds.
Format
Document (PDF)