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Novel Detection Techniques for Viable but Nonculturable Vibrio Vulnificus Cells in Response to Elevated Salinity
Title
Novel Detection Techniques for Viable but Nonculturable Vibrio Vulnificus Cells in Response to Elevated Salinity.
Creator
McHenry, Brandon, McCarthy, Peter J., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
Vibrio vulnificus is a marine pathogen of human health concern, capable of causing potentially fatal wound infections in a select group of the population. Previous studies have indicated this species’ strong negative correlation with salinity, not typically found above 30 ppt. This study assessed the ability of V. vulnificus to become Viable But Nonculturable in response to elevated salinity (35 ppt) as well as investigated novel methods for confirming their entrance into this state. Results...
Show moreVibrio vulnificus is a marine pathogen of human health concern, capable of causing potentially fatal wound infections in a select group of the population. Previous studies have indicated this species’ strong negative correlation with salinity, not typically found above 30 ppt. This study assessed the ability of V. vulnificus to become Viable But Nonculturable in response to elevated salinity (35 ppt) as well as investigated novel methods for confirming their entrance into this state. Results showed a complete loss of culturability in both Environmental and Clinical strains of this bacterium by 9 days after inoculation. Using a High Content Imager, it was determined that these pathogens were not dying (< 10%) in response to the treatment and were partially becoming cocci (≈35%). This study indicates the importance of understanding the impact environmental parameters have on this human pathogen, and what it means for reliably detecting them.
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Date Issued
2019
PURL
http://purl.flvc.org/fau/fd/FA00013240
Subject Headings
Vibrio vulnificus, Pathogenic microorganisms--Detection, Salinity
Format
Document (PDF)
The correlations between OSTDS sites and the pollution of the surface water bodies in Broward County
Title
The correlations between OSTDS sites and the pollution of the surface water bodies in Broward County.
Creator
Rocher, Yvel, Bloetscher, Frederick, Florida Atlantic University, College of Engineering and Computer Science, Department of Civil, Environmental and Geomatics Engineering
Abstract/Description
Sampling data of many surface water bodies in Broward County tend to reveal abnormal levels of bacterial concentrations for total coliform, Escherichia coli and Enterococcus. Given the presence of septic systems in geohydrologically sensitive areas throughout the County, several studies (Morin 2005 and Bocca 2007) suggested a correlation in the high bacterial densities and the septic sites.
Date Issued
2014
PURL
http://purl.flvc.org/fau/fd/FA00004324, http://purl.flvc.org/fau/fd/FA00004324
Subject Headings
Bacterial pollution of water, Groundwater -- Pollution, Pathogenic microorganisms -- Detection, Sewage disposal, Sewerage, Water -- Pollution -- Point source identification, Water quality -- Management
Format
Document (PDF)