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The potential for the marine biotechnology industry
Title
The potential for the marine biotechnology industry.
Creator
Pomponi, Shirley A.
Date Issued
1999
PURL
http://purl.flvc.org/FCLA/DT/3343835
Subject Headings
Marine biotechnology, Biotechnology, Pharmaceutical biotechnology
Format
Document (PDF)
In vitro biosynthetic studies of stevensine, a natural product from the sponge Teichaxinella morchella
Title
In vitro biosynthetic studies of stevensine, a natural product from the sponge Teichaxinella morchella.
Creator
Andrade, Paul., Florida Atlantic University, Kerr, Russell G.
Abstract/Description
Stevensine is a biologically active secondary metabolite produced by several marine sponges, including Teichaxinella morchella. Two in vitro methods were used to investigate the biosynthetic origin of stevensine in the sponge T. morchella. A cell-free enzyme extract was developed to test for the incorporation of the potential radiolabeled metabolic precursors histidine, arginine, ornithine, and proline. Secondly, cell cultures of T. morchella were used to determine the biosynthetic origin of...
Show moreStevensine is a biologically active secondary metabolite produced by several marine sponges, including Teichaxinella morchella. Two in vitro methods were used to investigate the biosynthetic origin of stevensine in the sponge T. morchella. A cell-free enzyme extract was developed to test for the incorporation of the potential radiolabeled metabolic precursors histidine, arginine, ornithine, and proline. Secondly, cell cultures of T. morchella were used to determine the biosynthetic origin of stevensine from the radiolabeled amino acids histidine, arginine, ornithine, and proline. Histidine and ornithine/proline were converted to stevensine in the cell culture system. This represents the first study involving the use of cell cultures of a marine invertebrate to investigate the biosynthesis of a biologically active natural product. An understanding of the biosynthetic process leading to the production of this compound in the source organism could lead to the development of more efficient and environmentally safer production methods.
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Date Issued
1999
PURL
http://purl.flvc.org/fcla/dt/15620
Subject Headings
Sponges, Marine biotechnology, Marine pharmacology
Format
Document (PDF)
Marine biotechnology: realizing the potential
Title
Marine biotechnology: realizing the potential.
Creator
Pomponi, Shirley A., Baden, Daniel G., Zohar, Y.
Date Issued
2007
PURL
http://purl.flvc.org/FCLA/DT/3338530
Subject Headings
Marine biotechnology, Marine pharmacology, Marine natural products
Format
Document (PDF)
Isolation and Structural Elucidation of Novel Bioactive Natural Products from Marine Organisms of the Western Atlantic Ocean
Title
Isolation and Structural Elucidation of Novel Bioactive Natural Products from Marine Organisms of the Western Atlantic Ocean.
Creator
Zhang, Long, West, Lyndon, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
Abstract/Description
The aim of this dissertation was to elaborate the exploration of biologically active secondary metabolites from the marine sponge Cacospongia cf. linteiformis collected from the Bahamas and the soft coral Briareum asbestinum collected from two different sites in Florida State, Boca Raton and Dry Tortugas. In chapter one, a review on previous chemical and biological studies of the marine sponge C. cf. linteiformis and soft coral B. asbestinum is provided. Particular attention is given to...
Show moreThe aim of this dissertation was to elaborate the exploration of biologically active secondary metabolites from the marine sponge Cacospongia cf. linteiformis collected from the Bahamas and the soft coral Briareum asbestinum collected from two different sites in Florida State, Boca Raton and Dry Tortugas. In chapter one, a review on previous chemical and biological studies of the marine sponge C. cf. linteiformis and soft coral B. asbestinum is provided. Particular attention is given to spongianolides and briarellins, two important classes of compounds isolated from C. cf. linteiformis and B. asbestinum, respectively, and their structural features and diverse bioactivities. In chapter two, the isolation and relative configuration determination of four epimeric sesterterpenoids, spongianolides E & F (18c, 18d, 19c, 19d) from C. cf. linteiformis collected from the Bahamas are discussed. Thanks to chemical modification (acetylation), diastereomeric 18c&18d and 19c&19d, respectively, were able to be isolated using chromatographic techniques for the first time, and then the relative configurations of 18c, 18d, 19c, 19d were determined based on NOESY NMR experiments. The bioactivity of mixture of compounds 18c, 18d, 19c, 19d were tested and it exhibited inhibition against Schnurri-3 (a regulator of postnatal bone mass). In chapter three, the isolation and structural elucidation of four new compounds, florellins A-D (49-52), from B. asbestinum collected off the coast of Boca Raton, FL are discussed. The molecular structures of these compounds were established by spectroscopic analysis. Compounds 49-52 are the first briarellins containing an acyl group at C-13, while 49 and 50 are the first briarellins possessing acylation at C-15. Florellins A–C (49-51) were screened and found cytotoxic against three human cell lines, BT474, WM266−4 and HEK293. In chapter four, the isolation and structural elucidation of four new compounds, florellins E-H (57-60), from B. asbestinum collected in Dry Tortugas, FL are discussed. The molecular structures of these compounds were established by spectroscopic analysis. Florellins F (58) and H (60) were screened against three human cell lines, BT474, WM266−4 and HEK293, but no cytotoxicity was exhibited. In chapter five, all the experimental procedures are described, including analytical instruments, animal materials, extraction and isolation processes, spectroscopic data and protocols of bioassays.
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Date Issued
2017
PURL
http://purl.flvc.org/fau/fd/FA00004850, http://purl.flvc.org/fau/fd/FA00004850
Subject Headings
Pharmacognosy., Natural products--Analysis., Marine pharmacology., Marine biotechnology., Marine algae--Biotechnology., Bioactive compounds.
Format
Document (PDF)
Isolation of marine natural products
Title
Isolation of marine natural products.
Creator
Wright, Amy E.
Date Issued
1998
PURL
http://purl.flvc.org/fau/fd/FA00007419
Subject Headings
Marine natural products, Bioactive compounds--Biotechnology
Format
Document (PDF)
Farming sponges for chemicals with pharmaceutical potential
Title
Farming sponges for chemicals with pharmaceutical potential.
Creator
Duckworth, Alan R., Harbor Branch Oceanographic Institute
Date Issued
2001
PURL
http://purl.flvc.org/FCLA/DT/3352965
Subject Headings
Sponges, Metabolites, Sponges--Biotechnology, Marine metabolites, Marine pharmacology
Format
Document (PDF)
The many faces of gene expression profiling: transcriptome analyses applied towardselucidating marine organismal interactions and metabolism
Title
The many faces of gene expression profiling: transcriptome analyses applied towardselucidating marine organismal interactions and metabolism.
Creator
Lopez, Jose V., Mouzon, B., McCarthy, Peter J., Kerr, Russell G.
Date Issued
2009
PURL
http://purl.flvc.org/fau/fd/FA00007286
Subject Headings
Gene expression, Transcriptome, Molecular biotechnology, Marine ecology, Marine organisms
Format
Document (PDF)
Quantitative assessment of marine sponge cells in vitro: Development of improved growth medium
Title
Quantitative assessment of marine sponge cells in vitro: Development of improved growth medium.
Creator
Willoughby, Robin, Pomponi, Shirley A.
Date Issued
2000
PURL
http://purl.flvc.org/FCLA/DT/2795606
Subject Headings
Animal cell biotechnology, Marine biology, Cell culture --Technique
Format
Document (PDF)
Therapeutic potential, mechanism of action, and ecology of novel marine natural products
Title
Therapeutic potential, mechanism of action, and ecology of novel marine natural products.
Creator
Winder, Priscilla L., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
Abstract/Description
The projects described in this dissertation are focused on compounds derived from the marine environment. Chapter 1 gives an introduction to the study of marine natural products to treat human ailments and a thorough review on compounds from lithistid sponges that have been isolated or synthesized since 2000. Chapter 2 describes the isolation and structure elucidation of two sesquiterpene substituted benzoquinone derivatives, petrosiquinones A and B, from a deep-water marine sponge from the...
Show moreThe projects described in this dissertation are focused on compounds derived from the marine environment. Chapter 1 gives an introduction to the study of marine natural products to treat human ailments and a thorough review on compounds from lithistid sponges that have been isolated or synthesized since 2000. Chapter 2 describes the isolation and structure elucidation of two sesquiterpene substituted benzoquinone derivatives, petrosiquinones A and B, from a deep-water marine sponge from the Family Petrosiidae. Although initially purified following activity in a (Sb(B-catenin/Tcf4 assay they were later followed using tumor cell line cytotoxicity assays. Petrosiquinone A was the more active of the two compounds with moderate cytotoxicity in the DLD-1, PANC-1, and AsPC-1 cell lines. In Chapter 3, the isolation and structure elucidation of two new marine-derived macrolides, madeirolide A and B, isolated from a deep-water lithistid sponge of the genus Leiodermatium is described., They were isolated using numerous chromatographic techniques and the structures were elucidated on the basis of 1D and 2D NMR spectra coupled with high resolution-mass spectrometry (HR-MS) data. Madeirolide A and B inhibited the growth of the fungal pathogen Candida albicans with minimum inhibitory concentrations (MIC) of 12.5 and 25 (So(Bg/mL, respectively, but were not cytotoxic in tumor cell assays under the conditions tested. Chapter 4 describes work performed to determine the molecular target of lasonolide A using affinity chromatography. The target of lasonolide A is of interest since lasonolide A is known to kill cancer cells in vitro through a unique mechanism., This chapter highlights the research performed to create an affinity matrix with immobilized lasonolide. A target has not been confirmed but there are a number of interesting hits that are being pursued. In Chapter 5, a liquid chromatography-mass spectrometry (LC-MS) screening method was established in order to rapidly identify the metabolites from numerous collections of Lyngbya spp. obtained from Broward and Lee County, Florida sites that may help marine ecologists assess the effects of Lyngbya spp. blooms on the environment. A link between the metabolites produced and nutrients from both the algal tissue and water column was also explored.
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Date Issued
2009
PURL
http://purl.flvc.org/FAU/369392
Subject Headings
Natural products, Therapeutic use, Sponges, Ecology, Marine resources, Research, Marine biotechnology
Format
Document (PDF)
Development of Methods for Separation and Characterization of Proteins from Gorgonian Sources
Title
Development of Methods for Separation and Characterization of Proteins from Gorgonian Sources.
Creator
Jett, Steven W., Florida Atlantic University, Kerr, Russell G., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
Abstract/Description
Research into the chemical constituents of marine organisms is a relatively new and growing field, given that much of the marine environment has only in the last half century become accessible. Despite the strides that have been made in ocean exploration, the marine biome has still been only barely explored. Much of the impetus for the isolation and identification of chemical entities from marine organisms has been related to their potential as medicines. For a number of reasons, including...
Show moreResearch into the chemical constituents of marine organisms is a relatively new and growing field, given that much of the marine environment has only in the last half century become accessible. Despite the strides that have been made in ocean exploration, the marine biome has still been only barely explored. Much of the impetus for the isolation and identification of chemical entities from marine organisms has been related to their potential as medicines. For a number of reasons, including synthetic efficiency, this has meant that many marine compounds isolated and studied have been small molecules. In recent years, large strides have been made in techniques for the isolation and identification of biological macromolecules, especially proteins. The understanding of these molecules, and their relationship to the biochemical processes of the marine organisms from which they have been isolated, is important not only because of the potential for this information to help in the synthesis of medicines, but because it may help in the recognition of processes that affect the very viability of marine organisms increasingly exposed to anthropogenic threats to their environment. This work consists of four related studies involving the development of methods for the separation and identification of proteins from a number or gorgon ian species. Chromatographic and gel based methods were used to isolate an elisabethatriene synthase (ELS) which shows promise as a biotechnological tool for the production or pseudopterosins. A number of gorgonians were screened for the presence of proteins that might have antimicrobial properties, and several organisms were identified that might be of interest in this context. Two-dimensional gel electrophoresis methods were then developed to allow the construction of gel maps for the azooxanthellate gorgonian Leptogorgia minimata and the xoozanthellae gorgonian Pseudopterogorgia e/isahethae, the separated proteins were digested and analyzed by LC/MSMS. and the information generated was used to examine the proteome of the organism for functional and phylogenetic relationships. Finally, the gorgonian EuniceafiShow less
Date Issued
2007
PURL
http://purl.flvc.org/fau/fd/FA00000862
Subject Headings
Marine biology, Pharmaceutical biotechnology, Proteins--Analysis, Gene expression--Research--Methodology, Biomolecules--Analysis
Format
Document (PDF)