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- Title
- GENOTYPIC SPERM SORTING: A less invasive “ART” to prevent Genetic Disorders in Newborns.
- Creator
- Adenmosun, Olumide O., Kumi-Diaka, James, Asghar, Waseem, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
- Abstract/Description
-
Genetic disorders like Cystic Fibrosis (CF) and X-linked Diseases (XLD) are inherited by offspring from parents who are healthy carriers of the autosomal recessive or allosomal genes. About 10-million Americans are healthy carriers of a mutant cysticfibrosis gene (predominantly F508del) and about 4% of newborns are at risk of being born with an X-linked disease. The current clinically approved mitigation plan for preventing genetic disorders in newborns from “at-risk couples” is to consider...
Show moreGenetic disorders like Cystic Fibrosis (CF) and X-linked Diseases (XLD) are inherited by offspring from parents who are healthy carriers of the autosomal recessive or allosomal genes. About 10-million Americans are healthy carriers of a mutant cysticfibrosis gene (predominantly F508del) and about 4% of newborns are at risk of being born with an X-linked disease. The current clinically approved mitigation plan for preventing genetic disorders in newborns from “at-risk couples” is to consider Preimplantation Genetic Testing for Monogenetic diseases (PGT-M). PGT-M involves an invasive microsurgical procedure that requires the removal of cells from 3-5day old embryos. To minimize this invasiveness, we proposed a less invasive approach to prevent genetic disorders in newborns by genotypically sorting sperm cells which may be used for fertilization events (IUI/IVF/ICSI) with specially characterized antigens on the sperm surface membrane. For the disease models being adopted in our study – CF and XLD; we utilized certain monoclonal antibodies (mab) to target the H-Y male antigen and the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein which are both selectively expressed on the sperm surface.
Show less - Date Issued
- 2021
- PURL
- http://purl.flvc.org/fau/fd/FA00013805
- Subject Headings
- Genetic disorders--Prevention, Genetic Testing, Reproductive technology, Cystic fibrosis
- Format
- Document (PDF)
- Title
- EFFECT OF N-METHYL-N'-NITRO-N-NITROSOGUANIDINE (MNNG) ON DNA OF CULTURED RAT HEPATOMA CELLS.
- Creator
- JHABVALA, PERSEUS, Florida Atlantic University, Stein, Abraham, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
The objective of this study was to elucidate the interaction of the carcinogen MNNG with nuclear DNA of Rat Hepatoma cells. The effect of a range of MNNG concentrations on RH cell DNA was studied by an analysis of the DNA fragments obtained in linear alkaline sucrose gradients. A macromolecular analysis of the sedimentation profile for 0.06 mM MNNG (Experiment I), and 0.1 mM MNNG (Experiment III), suggested that the system was paucidisperse and contained at least three components. The major...
Show moreThe objective of this study was to elucidate the interaction of the carcinogen MNNG with nuclear DNA of Rat Hepatoma cells. The effect of a range of MNNG concentrations on RH cell DNA was studied by an analysis of the DNA fragments obtained in linear alkaline sucrose gradients. A macromolecular analysis of the sedimentation profile for 0.06 mM MNNG (Experiment I), and 0.1 mM MNNG (Experiment III), suggested that the system was paucidisperse and contained at least three components. The major component Max 1 has a molecular weight comparable to that known for the mammalian replicon. Max 2 has a molecular weight twice that of Max 1 and Max 3 has a molecular weight half that of Max 1. The size of the replicon is comparable to that obtained by others. Inferences were drawn regarding the structure of chromatin and the role of the distribution of sites hypersensitive to methylation with respect to the oncogenes.
Show less - Date Issued
- 1985
- PURL
- http://purl.flvc.org/fcla/dt/14260
- Subject Headings
- Carcinogenicity testing, Cancer--Genetic aspects, DNA
- Format
- Document (PDF)