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- Title
- Immunoassay test strip for Microcystin-LR detection.
- Creator
- Xu, Jiesi., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
Microcystin-LR (MCLR) is hepatotoxic to animals and humans with disruption of liver structure causing cytoskeletal damage, necrosis and pooling of blood in the liver, leading to large increase in liver weight. It is also a strong liver tumor promoter and protein phosphatase inhibitor. Microcysin-LR binds protein phosphatases 1 and 2A, and influences regulation of cellular protein phosphorylation. In the present study, a colloidal gold based immunoassay test strip was developed for Microcystin...
Show moreMicrocystin-LR (MCLR) is hepatotoxic to animals and humans with disruption of liver structure causing cytoskeletal damage, necrosis and pooling of blood in the liver, leading to large increase in liver weight. It is also a strong liver tumor promoter and protein phosphatase inhibitor. Microcysin-LR binds protein phosphatases 1 and 2A, and influences regulation of cellular protein phosphorylation. In the present study, a colloidal gold based immunoassay test strip was developed for Microcystin-LR detection. The detection limit was found to be 1 ng/mL. 5 nm colloidal gold test strips exhibits more efficient for detection, compared with 20 nm colloidal gold test strips. The interaction between Microcystin-LR antibody (immunoglobulin G) and colloidal gold nanoparticles was investigated by various analytical methods, including Ultraviolet/Visible (UV/VIS), Fourier Transform Infrared (FTIR) and Fluorescence spectroscopy as well as transmission electron microscopy (TEM).
Show less - Date Issued
- 2010
- PURL
- http://purl.flvc.org/FAU/2683532
- Subject Headings
- Immunoassay, Biosensors, Environmental chemistry, Cyanobacterial toxins, Drinking water, Microbiology
- Format
- Document (PDF)
- Title
- Plastic Debris in the Marine Environment: Fate, Fragmentation, and Niche Opportunities of the Plastisphere.
- Creator
- Bos, Ryan P., Mincer, Tracy J., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
- Abstract/Description
-
The goal of this dissertation was to examine the fate and fragmentation of plastic debris in the marine environment and characterize the microbial communities colonizing naturally occurring substrates and geologically recent plastic inputs in the ocean using analytical chemistry and genomic techniques. Altogether, the data presented herein reveal the presence of heretofore undescribed plastic accumulation zones in the ocean and depict a stark contrast in microbial traits between early and...
Show moreThe goal of this dissertation was to examine the fate and fragmentation of plastic debris in the marine environment and characterize the microbial communities colonizing naturally occurring substrates and geologically recent plastic inputs in the ocean using analytical chemistry and genomic techniques. Altogether, the data presented herein reveal the presence of heretofore undescribed plastic accumulation zones in the ocean and depict a stark contrast in microbial traits between early and mature plastic biofilm stages. These data further suggest that emergent plastic biofilm properties can be forecasted across environmental gradients, with the largescale genomic characteristics of early colonizers varying little across conditions. Chapter I of this thesis is an introduction to the current body of work regarding the plastisphere. Chapter II explores the ‘cradle to grave’ fragmentation, transformation, and transport of model microplastic particles and single-use plastic items in an artificial beach setting. Chapter III presents the first metagenomic insights into early biofilm formation on virgin microplastic surfaces in the marine environment and how early colonizers self-assemble, compared to mature, taxonomically, and metabolically diverse biofilms residing on free-drifting plastic pollution. Chapter IV further investigates microbial adaptations for initial colonization of virgin control and plastic surfaces and examines biofilm assemblage dynamics by employing metagenomics on a 16-day time series in a wastewater treatment facility. Chapter V synthesizes observations from the previous core chapters and discusses what these findings mean in a broader ecological and evolutionary perspective. Appendix 1 is a reprint of the manuscript describing the distribution of microplastics beneath the inner and outer plastic accumulation zones of the South Atlantic Subtropical Gyre. Appendix 2 is a submitted manuscript detailing the isolation, characterization, and selective adaptations of Vibrio bacteria colonizing eel leptocephali, free-drifting plastic pollution, Sargassum, and seawater in the North Atlantic Ocean.
Show less - Date Issued
- 2023
- PURL
- http://purl.flvc.org/fau/fd/FA00014127
- Subject Headings
- Plastic scrap, Plastic scrap--Environmental aspects, Biofilms, Fragmentation (Chemistry)
- Format
- Document (PDF)
- Title
- Recovering Titanium Dioxide (TiO2) after its Use to Treat Leachate for Reuse on Future Leachate Flows.
- Creator
- Coffman, Neil, Meeroff, Daniel E., Florida Atlantic University, College of Engineering and Computer Science, Department of Civil, Environmental and Geomatics Engineering
- Abstract/Description
-
This thesis was about finding a recovery method for TiO2, using a TiO2 recovery technology, which was high enough to be economical ($10 - $15 per 1,000 gallons) to be adopted by wastewater treatment plants. When comparing recovery technologies, the top three which were investigated further through experimentation were a centrifuge, sedimentation tank, and microfilter membrane. Upon experimentation and research, the TiO2 recovery efficiencies of these technologies were 99.5%, 92.5%, and 96.3%,...
Show moreThis thesis was about finding a recovery method for TiO2, using a TiO2 recovery technology, which was high enough to be economical ($10 - $15 per 1,000 gallons) to be adopted by wastewater treatment plants. When comparing recovery technologies, the top three which were investigated further through experimentation were a centrifuge, sedimentation tank, and microfilter membrane. Upon experimentation and research, the TiO2 recovery efficiencies of these technologies were 99.5%, 92.5%, and 96.3%, respectively. When doing economic analysis on these technologies comparing TiO2 efficiencies and capital and operational costs, the centrifuge was the most preferred economic option. Also, its cost did were in the economical range ($10 - $15/1,000 gallons) which makes even this technology economical. Besides that, important and valuable information about TiO2: settling behavior, particle size and zeta potential, interactions with COD, and filter operations (particle characterization) were discovered for future research and future testing on this issue.
Show less - Date Issued
- 2015
- PURL
- http://purl.flvc.org/fau/fd/FA00004490, http://purl.flvc.org/fau/fd/FA00004490
- Subject Headings
- Environmental chemistry, Environmental engineering, Fluid dynamics, Microfluidic devices, Sewage disposal plants -- Management, Solution (Chemistry), Titanium dioxide -- Environmental aspects, Titanium dioxide -- Industrial applications
- Format
- Document (PDF)
- Title
- Experimental evaluation of the durability of fly ash-based geopolymer concrete in the marine environment.
- Creator
- Edouard, Jean-Baptiste., College of Engineering and Computer Science, Department of Civil, Environmental and Geomatics Engineering
- Abstract/Description
-
The construction industry is increasingly turning to the use of environmentally friendly materials in order to meet the sustainable aspect required by modern infrastructures. Consequently, for the last two decades, the expansion of this concept, and the increasing global warming have raised concerns on the extensive use of Portland cement due to the high amount of carbon dioxide gas associated with its production. The development of geopolymer concretes offers promising signs for a change in...
Show moreThe construction industry is increasingly turning to the use of environmentally friendly materials in order to meet the sustainable aspect required by modern infrastructures. Consequently, for the last two decades, the expansion of this concept, and the increasing global warming have raised concerns on the extensive use of Portland cement due to the high amount of carbon dioxide gas associated with its production. The development of geopolymer concretes offers promising signs for a change in the way of producing concrete. However, to seriously consider geopolymer binders as an alternative to ordinary Portland cement, the durability of this new material should be evaluated in any comparative analysis. The main purpose of this study was to evaluate the durability characteristics of low calcium fly ash-based geopolymer concretes subjected to the marine environment, compared to ordinary Portland cement concrete with similar exposure. To achieve this goal, 8 molar geopolymer, 14 molar geopolymer and ordinary Portland cement concrete mixes were prepared and tested for exposure in seawater. Compressive strengths in the range of 2900 to 8700 psi (20-60 MPa) were obtained. The corrosion resistance performance of steel-reinforced concrete beams, made of these mixes, was also studied, using an accelerated electrochemical method, with submergence in salt water. The test results indicated that the geopolymer concrete showed excellent resistance to chloride attack, with longer time to corrosion cracking, compared to ordinary Portland cement concrete.
Show less - Date Issued
- 2011
- PURL
- http://purl.flvc.org/FAU/3170960
- Subject Headings
- Reinforced concrete, Corrosion, Testing, Reinforced concrete construction, Concrete, Mixing, Quality control, Environmental chemistry, Industrial applications, Polymer composites
- Format
- Document (PDF)