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Queen conch (Strombus gigas) culture techniques forresearch, stock enhancement and growout markets
Title
Queen conch (Strombus gigas) culture techniques forresearch, stock enhancement and growout markets.
Creator
Davis, Megan
Date Issued
2000
PURL
http://purl.flvc.org/fau/fd/FA00007152
Subject Headings
Queen conch, Strombus gigas, Aquaculture, Culture technique
Format
Document (PDF)
Closed tubular cultivators: an innovative system for commercial culture of microalgae
Title
Closed tubular cultivators: an innovative system for commercial culture of microalgae.
Creator
Spektorova, L., Creswell, R. LeRoy, Vaughan, David E., Harbor Branch Oceanographic Institute
Date Issued
1997
PURL
http://purl.flvc.org/fau/fd/FA00007418
Subject Headings
Microalgae--Cultures and culture media, Algae culture--Technique, Aquaculture
Format
Document (PDF)
Quantitative assessment of marine sponge cells in vitro: Development of improved growth medium
Title
Quantitative assessment of marine sponge cells in vitro: Development of improved growth medium.
Creator
Willoughby, Robin, Pomponi, Shirley A.
Date Issued
2000
PURL
http://purl.flvc.org/FCLA/DT/2795606
Subject Headings
Animal cell biotechnology, Marine biology, Cell culture --Technique
Format
Document (PDF)
Scenographia
Title
Scenographia: investigations of line, light and the theatricality in the micro-scale.
Creator
Matsi, Sofâia., Dorothy F. Schmidt College of Arts and Letters, Department of Visual Arts and Art History
Abstract/Description
Informed by my training in the physical sciences, my thesis show presents results of research on the elements of line and dramatic staging. My process is documented through the experimental grounds of my sketchbooks. Originating from observational drawings of organic forms, my fascination with line quality and my desire for theatrical settings propelled these drawings to acquire a three dimensional presence. Through constructed book formats, staged micro-scenes, and photographs, I test my...
Show moreInformed by my training in the physical sciences, my thesis show presents results of research on the elements of line and dramatic staging. My process is documented through the experimental grounds of my sketchbooks. Originating from observational drawings of organic forms, my fascination with line quality and my desire for theatrical settings propelled these drawings to acquire a three dimensional presence. Through constructed book formats, staged micro-scenes, and photographs, I test my extracted, abstract forms in varying intimate environments. I control these worlds through scale, space and lighting, and I observe and record how they behave. I gather data through a methodical process that is infused with the empirical instincts I developed as a scientist. I express the resulting distilled organic forms in the most theatrical way I can invent.
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Date Issued
2011
PURL
http://purl.flvc.org/FAU/3171721
Subject Headings
Art and technology, Popular culture, Drawing, Technique, Installations (Art)
Format
Document (PDF)
ADVANCING THE CULTIVABILITY OF SOIL BACTERIA USING A DYNAMIC SOIL ENVIRONMENT AND SOIL EXTRACT METHOD
Title
ADVANCING THE CULTIVABILITY OF SOIL BACTERIA USING A DYNAMIC SOIL ENVIRONMENT AND SOIL EXTRACT METHOD.
Creator
España, Erick, Brooks, Randy W., Esiobu, Nwadiuto, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
Bacteria are inarguably the most ubiquitous and adaptive organisms on the planet. The vast, diverse community of microbes residing in soil are mostly studied using sequencing technologies because over 99% of them are currently uncultivable in the laboratory. This lack of diverse bacterial cultivation presents a serious challenge for modern microbiological and medical science where the discovery of novel antibiotic producers and microbial products has been outpaced by the rise in drug...
Show moreBacteria are inarguably the most ubiquitous and adaptive organisms on the planet. The vast, diverse community of microbes residing in soil are mostly studied using sequencing technologies because over 99% of them are currently uncultivable in the laboratory. This lack of diverse bacterial cultivation presents a serious challenge for modern microbiological and medical science where the discovery of novel antibiotic producers and microbial products has been outpaced by the rise in drug resistance. This study designed and tested two new cost-effective culture systems called the “Dynamic Soil Environment” and Soil Extract Systems with the goal of increasing the cultivable communities of diverse bacteria in a soil sample over standard methods. Illumina MiSeq sequencing and DADA2 pipeline protocols were used to analyze community DNA from cultivated samples and source soil metagenomes. Autoclaved soil extract media in the Soil Extract Experiment yielded a statistically significantly greater Shannon’s (p = 0.008) and Simpson’s diversity (p = 0.007) of bacteria over pH modified (6.4) nutrient agar media over 30 days of incubation. Autoclaved soil extract media was also able to cultivate, on average, 33% of species in bulk soil sequences compared to 27% from standard nutrient agar however these differences weren’t statistically significant. The length of incubation had a lesser effect than media type on yield of bacteria over 30 days in batch culture conditions. Species richness and diversity generally decreased over time except in soil extract samples. In the Dynamic Soil Environment experiment, membrane plates placed on a live soil environment produced a slightly higher diversity than autoclaved membrane plates and control plates without soil, however, these differences were not statistically significant except when analyzed with Chao1 diversity (0.041). Cultivated bacterial diversity and communities differed more according to media type than soil environment with statistically significant differences between standard and pH modified nutrient agar. Media with a 5.8 pH buffer produced a significantly higher relative abundance of the well-known antibiotic-producers, Actinobacteria (t(10) = -5.715, p < .000) and also Proteobacteria (t(10) = -10.127, p < .000). This study establishes cost-effective methods of cultivating more diverse bacterial communities for low-funded laboratories. Culture conditions for the reliable cultivation of higher relative abundances of bacterial groups belonging to Actinobacteria and Proteobacteria are also established with the Dynamic Soil Environment Experiment.
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Date Issued
2019
PURL
http://purl.flvc.org/fau/fd/FA00013304
Subject Headings
Bacteriological Techniques--methods, Bacteriology--Cultures and culture media, Soil
Format
Document (PDF)
Microalgae culture protocol
Title
Microalgae culture protocol.
Creator
Jaramillo, Juan C.
PURL
http://purl.flvc.org/fcla/dt/3359270
Subject Headings
Microalgae--Cultures and culture media, Microalgae--Handbooks, manuals, etc., Laboratory Techniques and Procedures, Laboratory Techniques and Procedures--Laboratory Manuals
Format
Document (PDF)