Current Search: Cells -- Motility (x)
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- Title
- Knockdown of KIF9 leads to defects in mitotic entry and progression in mammalian cells.
- Creator
- Alsina, Laura., Harriet L. Wilkes Honors College
- Abstract/Description
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Kinesin motors bind to microtubules and function in mitosis and intracellular transport depending on the position of the motor domain within the primary sequence (Hirokawa and Noda 2008). KIF9 has recently been shown to be involved in MTOC positioning and mitotic entry in Dictyostelium (Tikhonenko et al. 2009). To determine if a similar role for KIF9 exists in mammalian cells, we are using siRNA-mediated knockdown of KIF9 in COS-7 cells. Analysis of unsynchronized and cell-cycle synchronized...
Show moreKinesin motors bind to microtubules and function in mitosis and intracellular transport depending on the position of the motor domain within the primary sequence (Hirokawa and Noda 2008). KIF9 has recently been shown to be involved in MTOC positioning and mitotic entry in Dictyostelium (Tikhonenko et al. 2009). To determine if a similar role for KIF9 exists in mammalian cells, we are using siRNA-mediated knockdown of KIF9 in COS-7 cells. Analysis of unsynchronized and cell-cycle synchronized cells treated with siRNA to KIF9 reveal that the transition from G2 to M phase is delayed and that mitotic progression is also affected. Additionally, our data indicates that spindle pole function during anaphase may be abnormal in cells treated with siRNA, suggesting a role for KIF9 during that stage.
Show less - Date Issued
- 2010
- PURL
- http://purl.flvc.org/FAU/3334255
- Subject Headings
- Cells, Motility, Protoplasmic streaming, Cell organelles, Formation, Cellular signal transduction
- Format
- Document (PDF)
- Title
- Adopting the orphan: determining the role of the motor protein KIF9 during the cell cycle.
- Creator
- Rivera Rios, Miguel E., Harriet L. Wilkes Honors College
- Abstract/Description
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The kinesin superfamily of microtubule motor proteins is subdivided into families based upon structure and function. KIF9 is the founding member of the Kinesin-9 family, which is a largely uncharacterized group of kinesins. It was originally identified by sequence homology to other kinesins. Subsequent studies have shown that KIF9 interacts with proteins involved in cell shape remodeling, cell migration and proper centrosomal positioning. We have examined KIF9 function in mammalian cells...
Show moreThe kinesin superfamily of microtubule motor proteins is subdivided into families based upon structure and function. KIF9 is the founding member of the Kinesin-9 family, which is a largely uncharacterized group of kinesins. It was originally identified by sequence homology to other kinesins. Subsequent studies have shown that KIF9 interacts with proteins involved in cell shape remodeling, cell migration and proper centrosomal positioning. We have examined KIF9 function in mammalian cells using shRNA-mediated knockdown and GFP-plasmid overexpression. By knocking dow KIF9 expression in these cells, we have seen several effects on normal cell cycle progression. Using various cell cycle markers, we have observed a decrease in the number of cells in late S phase. In addition, there is a marked increase in the number of cells in early mitosis in unexpected time intervals. We propose that KIF9 is required for proper cell progression, via a potentially novel checkpoint mechanism.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3359321, http://purl.flvc.org/fau/fd/FADT3359321
- Subject Headings
- Cell organelles, Formation, Cellular signal transduction, Protoplasmic streaming, Cells, Motility, Cell division, Research
- Format
- Document (PDF)
- Title
- Characterization of the motor protein, KIF9, in mammalian cell mitotic progression.
- Creator
- Hoke, Jordan, Harriet L. Wilkes Honors College
- Abstract/Description
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The kinesin family of microtubule motors is divided into subfamilies based on structure and function. KIF9, founder of the Kinesin-9 family, has been found to interact with the GTPase Gem. Subsequent studies have shown that KIF9 is vital for flagellar movement and podosome regulation. Previous work has proposed KIF9 is required for microtubule organization as well as proper mitotic entry, progression and completion. In this study, I examined the function of KIF0 in mitotic progression using...
Show moreThe kinesin family of microtubule motors is divided into subfamilies based on structure and function. KIF9, founder of the Kinesin-9 family, has been found to interact with the GTPase Gem. Subsequent studies have shown that KIF9 is vital for flagellar movement and podosome regulation. Previous work has proposed KIF9 is required for microtubule organization as well as proper mitotic entry, progression and completion. In this study, I examined the function of KIF0 in mitotic progression using shRNA-mediated knockdown, and overexpression. In knockdown cells, I saw a significant delay in mitotic progression as well as an increase in multipolarity and multinuclearity, suggesting a failure of cytokinesis. Overexpression of KIF9 produced similar effects on mitotic progression, as well as a marked increase in chromosome distance during anaphase. Taken with previous results, my research indicated that KIF9 is required for normal mitotic progression and completion, possible via regulation of the contractile ring.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3359298
- Subject Headings
- Cells, Motility, Protoplasmic streaming, Cell organelles, Cellular signal transduction, Cytoskeletal proteins
- Format
- Document (PDF)
- Title
- The Effect of Fluid Flow on Human Sperm Fertility.
- Creator
- Rappa, Kari, Asghar, Waseem, Florida Atlantic University, College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science
- Abstract/Description
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Current sperm processing methods used in assisted reproductive technologies can cause damage to the sperm cell. New ways that mimic the natural guidance mechanisms present in the female genital tract may offer ways to sort sperm with better fertility parameters. Sperm that respond to these cues may have better over sperm health. Human sperm exhibit positive rheotaxis by orienting and swimming against the fluid released by the female genital tract. At certain flow rates sperm can actively...
Show moreCurrent sperm processing methods used in assisted reproductive technologies can cause damage to the sperm cell. New ways that mimic the natural guidance mechanisms present in the female genital tract may offer ways to sort sperm with better fertility parameters. Sperm that respond to these cues may have better over sperm health. Human sperm exhibit positive rheotaxis by orienting and swimming against the fluid released by the female genital tract. At certain flow rates sperm can actively orient and swim against the flow. Sperm retrieved that exhibit positive rheotaxis have higher motility and better morphology than the original semen sample.
Show less - Date Issued
- 2017
- PURL
- http://purl.flvc.org/fau/fd/FA00004938, http://purl.flvc.org/fau/fd/FA00004938
- Subject Headings
- Human reproduction--Immunological aspects., Fertility, Human., Cell separation., Reproductive health., Cilia and ciliary motion., Spermatozoa--Motility.
- Format
- Document (PDF)