View All Items
(1 - 20 of 501)
Pages
- Title
- An unrecognized ancient lineage of green plants persists in deep marine waters.
- Creator
- Zechman, F. W., Verbruggen, H., Leliaert, F., Ashworth, M., Buchheim, M. A., Fawley, M. W., Spalding, H., Pueschel, C. M., Buchheim, J. A., Verghese, B., Hanisak, M. Dennis
- Date Issued
- 2010
- PURL
- http://purl.flvc.org/FCLA/DT/3174108
- Subject Headings
- Marine plants, Marine flora
- Format
- Document (PDF)
- Title
- Genetics of Selected Marine Bacteria.
- Creator
- Pursglove, Diana L., Waddell, Glenn H., Florida Atlantic University
- Abstract/Description
-
Marine bacteria are usually described as those bacteria which grow optimally in sea water. Their complex requirements for specific ions (sodium, potassium and magnesium) have been used to differentiate marine from terrigenous bacteria. These requirements, however, vary at the gene level through mutation. Both spontaneous and induced (ultraviolet light) mutations to loss of the ion requirement were observed. Spontaneous frequencies were low (below 10^-6); induced frequencies, high (10^-1 to 10...
Show moreMarine bacteria are usually described as those bacteria which grow optimally in sea water. Their complex requirements for specific ions (sodium, potassium and magnesium) have been used to differentiate marine from terrigenous bacteria. These requirements, however, vary at the gene level through mutation. Both spontaneous and induced (ultraviolet light) mutations to loss of the ion requirement were observed. Spontaneous frequencies were low (below 10^-6); induced frequencies, high (10^-1 to 10^-8). Back mutations were noticed. A stepwise mechanism was postulated for the mechanism of the mutation involved in the appearance of ion independent bacteria in a population of cells. Genetic analysis of the mechanism by which variations in ion requirements occurred included determination of base ratios and of transformation frequencies. The base ratios of mutant and parent bacteria were similar enough and the transformation frequencies high enough (10^-3 to 10^-5) to show a close relationship between the two types of bacteria. This similarity indicated that, although the genome of marine bacteria, which clearly controls the requirement for specific ions from the values of mutation frequencies, is altered by the action of ultraviolet light, the change was not observable by the methods used. The change could have been induced on the gene level to effect the synthesis of structural or functional proteins (enzymes).
Show less - Date Issued
- 1968
- PURL
- http://purl.flvc.org/fau/fd/FA00000814
- Subject Headings
- Marine microbiology, Marine bacteria
- Format
- Document (PDF)
- Title
- Marine biotechnology: realizing the potential.
- Creator
- Pomponi, Shirley A., Baden, Daniel G., Zohar, Y.
- Date Issued
- 2007
- PURL
- http://purl.flvc.org/FCLA/DT/3338530
- Subject Headings
- Marine biotechnology, Marine pharmacology, Marine natural products
- Format
- Document (PDF)
- Title
- Carbon Balance Studies on Selected Species of Marine Filamentous Fungi.
- Creator
- Dias, Anthony P., Sguros, Peter L., Florida Atlantic University
- Abstract/Description
-
Selected species of marine filamentous fungi, Halosphaeria mediosetigera, Humicola alopallonella, Culcitalna achraspora and Orbimyces spectabilis, grown in a semi-synthetic medium containing glucose 0.5, ammonium nitrate 0.24, tris(hydroxyrnethyl)aminomethane 0.12 and yeast extract 0.1% (Medium A) and modifications thereof have been studied with regard to cellular composition, carbon fate and growth kinetics. Cultures were aerated with sterile, carbon dioxide-free air and washed mycelia were...
Show moreSelected species of marine filamentous fungi, Halosphaeria mediosetigera, Humicola alopallonella, Culcitalna achraspora and Orbimyces spectabilis, grown in a semi-synthetic medium containing glucose 0.5, ammonium nitrate 0.24, tris(hydroxyrnethyl)aminomethane 0.12 and yeast extract 0.1% (Medium A) and modifications thereof have been studied with regard to cellular composition, carbon fate and growth kinetics. Cultures were aerated with sterile, carbon dioxide-free air and washed mycelia were analyzed for macroconstituent elementary composition, total carbon, protein, chitin and lipids. Culture filtrates were tested for total carbon, .extracellular carbohydrates and organic acids. Physiological profiles were obtained to ascertain and correlate the ·extent and rate of growth; and changes in pH, concentration of glucose . and anthronepositive materials. · Elementary analyses of washed fungal mycelia showed 41.0 to 49.0% carbon, 4.7 to 6.0% nitrogen and 5.4 to 6.5% · hydrogen. Optimal growth was accompanied by a concomitant fall in pH followed by a shift back to the alkaline side. Following autolysis at 8 days, almost one-third of the glucose substrate reappeared in the medium accompanied by smaller amounts of mannose with Halosphaeria. With Culcitalna, following exhaustion of glucose in the medium, autolysis was accompanied by the reappearance of glucose. Pentoses and a hexose other than glucose also were indicated by relative mobilities on thin layer chromatograms. The most profound pH changes were obtained with Humicola reaching a low of 2.4 in 15 days, but the pH at 45 days had stabilized around 5.3. Increasing the glucose concentration to 5.0% (Medium B) did not increase the mycelial yields to any substantial degree except for Halosphaeria. In all cases pH fluctuated around 4 between 10 and 25 days, hut the final pH was about 6. Production of mycelia was always accompanied by substantial utilization of the glucose in the medium. When the glucose concentration was raised to 5%, to force the buildup of metabolic products, and yeast extract omitted (Medium C), substantial amounts of glucose were utilized, but this was not manifested by parallel increases in growth. Carbon balance studies confirmed that pH changes were not due to the production of organic acids, but probably to preferential utilization of ailllllonium cations. The initial substrate carbon was found almost exclusively in the fungal mycelia and evolved carbon dioxide. Carbon recoveries obtained under standard conditions were 97.9, 97.4, 98.2 and 94.0% respectively for Halosphaeria mediosetigera, Humicola alopallonella, Culcitalna achraspora and Orbimyces spectabilis in the standard medium containing 0.5% glucose. In 5% glucose (Medium B) the recoveries were 93.0, 90.6 and 84.7% for the first three organisms mentioned. Time did not allow a complete investigation of the causes. of decreased carbon recoveries, but greater production of incompletely oxidized metabolites appeared probable. Economic coefficients were determined for three species in three media while determinations on the fourth species are in progress. Culcitalna gave values of 71 in Medium A, 40 in Medium B and 3 in Medium C; Halosphaeria gave 78 in A, 45 in B and 15 in C; Orbimyces gave 75 in A and 7 in C . The effect of omission of yeast extract and of elevated glucose concentration on the economic coeffic ients was pronounced . The only products detected under exaggerated carbon conditions were acetic acid in the culture broth of Culcitalna and a greenish pigment in the culture filtrate of Halospaeria. The analogously-suggested accumulation of 2-ketogluconic acid in the latter case could be indicative of the functioning of the hexose monophosphate shunt or another primary aerobic pathway.
Show less - Date Issued
- 1970
- PURL
- http://purl.flvc.org/fau/fd/FA00000743
- Subject Headings
- Marine fungi
- Format
- Document (PDF)
- Title
- EVIDENCE FOR THE EXISTENCE OF THE TRICARBOXYLIC ACID CYCLE AND GLYOXYLATE PATHWAY IN CERTAIN MARINE FILAMENTOUS FUNGI.
- Creator
- VEMBU, DEVI T., Florida Atlantic University
- Abstract/Description
-
Cellulolytic marine isolates, Culcitalna achraspora and Halosphaeria mediosetigera, were shaker-grown at 25 C in artificial seawater containing glucose, NH4NO3 , tris(hydroxymethyl) aminomethane and yeast extract, pH 7.5. Cultures were harvested in the late linear phase where whole cell endogenous respiration was maximal. Spectrophotometric determinations on acetone powder extracts revealed the presence of aconitase, NAD-linked isocitrate dehydrogenase, succinate dehydrogenase, fumarase,...
Show moreCellulolytic marine isolates, Culcitalna achraspora and Halosphaeria mediosetigera, were shaker-grown at 25 C in artificial seawater containing glucose, NH4NO3 , tris(hydroxymethyl) aminomethane and yeast extract, pH 7.5. Cultures were harvested in the late linear phase where whole cell endogenous respiration was maximal. Spectrophotometric determinations on acetone powder extracts revealed the presence of aconitase, NAD-linked isocitrate dehydrogenase, succinate dehydrogenase, fumarase, malate dehydrogenase and isocitratase. Culcitalna also showed NADP-linked isocitrate dehydrogenase activity. Manometric examinations of acetone powder and dialyzed sonic extracts were largely confirmatory. Oxidation of citric acid cycle intermediates by extracts were observed with Culcitalna for all but succinate with sonic extracts and for all but succinate and 2-oxoglutarate in acetone powder extracts. With Halosphaeria, neither extract could oxidize citrate or succinate, while acetone powder extracts were unable to degrade cis-aconitate and 2-oxoglutarate. Native mycelia were active only against malate. Attempts to permeate cell walls with ethyl esters of intermediates failed.
Show less - Date Issued
- 1972
- PURL
- http://purl.flvc.org/fcla/dt/13478
- Subject Headings
- Marine fungi
- Format
- Document (PDF)
- Title
- Isolation of Latrunculin A, 6,7-Epoxylatrunculin A, Fijianolide A, and Euryfuran from a new genus of the family Thorectidae.
- Creator
- Gulavita, N. K., Gunasekera, Sarath P., Pomponi, Shirley A.
- Date Issued
- 1992
- PURL
- http://purl.flvc.org/FCLA/DT/3332996
- Subject Headings
- Sponges, Marine toxins, Marine metabolites
- Format
- Document (PDF)
- Title
- In vitro biosynthetic studies of stevensine, a natural product from the sponge Teichaxinella morchella.
- Creator
- Andrade, Paul., Florida Atlantic University, Kerr, Russell G.
- Abstract/Description
-
Stevensine is a biologically active secondary metabolite produced by several marine sponges, including Teichaxinella morchella. Two in vitro methods were used to investigate the biosynthetic origin of stevensine in the sponge T. morchella. A cell-free enzyme extract was developed to test for the incorporation of the potential radiolabeled metabolic precursors histidine, arginine, ornithine, and proline. Secondly, cell cultures of T. morchella were used to determine the biosynthetic origin of...
Show moreStevensine is a biologically active secondary metabolite produced by several marine sponges, including Teichaxinella morchella. Two in vitro methods were used to investigate the biosynthetic origin of stevensine in the sponge T. morchella. A cell-free enzyme extract was developed to test for the incorporation of the potential radiolabeled metabolic precursors histidine, arginine, ornithine, and proline. Secondly, cell cultures of T. morchella were used to determine the biosynthetic origin of stevensine from the radiolabeled amino acids histidine, arginine, ornithine, and proline. Histidine and ornithine/proline were converted to stevensine in the cell culture system. This represents the first study involving the use of cell cultures of a marine invertebrate to investigate the biosynthesis of a biologically active natural product. An understanding of the biosynthetic process leading to the production of this compound in the source organism could lead to the development of more efficient and environmentally safer production methods.
Show less - Date Issued
- 1999
- PURL
- http://purl.flvc.org/fcla/dt/15620
- Subject Headings
- Sponges, Marine biotechnology, Marine pharmacology
- Format
- Document (PDF)
- Title
- A marine biological sampling device (MBSD).
- Creator
- Clark, A. M.
- Date Issued
- 1985-10-28
- PURL
- http://purl.flvc.org/fcla/dt/3358982
- Subject Headings
- Sampling, Marine biology, Marine biology--Research
- Format
- Document (PDF)
- Title
- Mathematical modeling of plankton patchiness.
- Creator
- Ather, Simantha S., Noonburg, Erik G., Graduate College
- Date Issued
- 2011-04-08
- PURL
- http://purl.flvc.org/fcla/dt/3164458
- Subject Headings
- Marine plankton, Marine zooplankton, Mathematical models
- Format
- Document (PDF)
- Title
- The struggle of the marine workers.
- Creator
- Sparks, N.
- Date Issued
- 1930
- PURL
- http://purl.flvc.org/FCLA/DT/3336803
- Subject Headings
- Merchant marine -- United States., Marine workers.
- Format
- Document (PDF)
- Title
- In memoriam: John Hood Ryther 1922-2006.
- Creator
- Mann, Roger, Yentsch, Charles S., Lapointe, Brian E.
- Date Issued
- 2007
- PURL
- http://purl.flvc.org/FCLA/DT/3174063
- Subject Headings
- Plankton, Marine plankton, Marine biology, Biological oceanography
- Format
- Document (PDF)
- Title
- Inland marine fish culture in low salinity recirculating aquaculture systems.
- Creator
- Riche, Marty A., Pfeiffer, Timothy J., Wills, Paul S., Amberg, J. J., Sepúlveda, M. S.
- Date Issued
- 2012
- PURL
- http://purl.flvc.org/FCLA/DT/3343851
- Subject Headings
- Marine aquaculture, Mariculture, Marine fishes, Salinity
- Format
- Document (PDF)
- Title
- A new bastadin from the sponge Psammaplysilla purpurea.
- Creator
- Carney, J. R., Scheuer, P. J., Kelly-Borges, M., Harbor Branch Oceanographic Institute
- Date Issued
- 1993
- PURL
- http://purl.flvc.org/FCLA/DT/3318955
- Subject Headings
- Sponges --Research, Marine metabolites, Marine natural products
- Format
- Document (PDF)
- Title
- Isolation and structure elucidation of onnamide a, a new bioactive metabolite of a marine sponge, Theonella sp.
- Creator
- Sakemi, S., Ichiba, T., Kohmoto, S., Saucy, G., Higa, T., Harbor Branch Oceanographic Institute
- Date Issued
- 1988
- PURL
- http://purl.flvc.org/FCLA/DT/3332945
- Subject Headings
- Sponges, Bioactive compounds, Marine metabolites, Marine Toxins
- Format
- Document (PDF)
- Title
- Bioactivity of marine organisms: relationships with taxonomy, geography and depth.
- Creator
- Reed, John K., Sennett, Susan H., McCarthy, Peter J., Pitts, Tara P., Wright, Amy E., Pomponi, Shirley A.
- Date Issued
- 1998
- PURL
- http://purl.flvc.org/fau/fd/FA00007421
- Subject Headings
- Marine organisms, Bioactive compounds, Marine natural products
- Format
- Document (PDF)
- Title
- Three new bromotyrosine derived metabolites of the sponge Psammaplysilla purpurea.
- Creator
- Jurek, J., Yoshida, W. Y., Scheuer, P. J., Kelly-Borges, M., Harbor Branch Oceanographic Institute
- Date Issued
- 1993
- PURL
- http://purl.flvc.org/FCLA/DT/3318965
- Subject Headings
- Marine natural products, Sponges --Research, Marine metabolites
- Format
- Document (PDF)
- Title
- Marine organisms as a source of novel lead structures for drug development.
- Creator
- Wright, Amy E.
- Date Issued
- 2000
- PURL
- http://purl.flvc.org/fau/fd/FA00007060
- Subject Headings
- Marine organisms, Marine natural products, Drug development
- Format
- Document (PDF)
- Title
- The oceans and human health: the discovery and development of marine-derived drugs.
- Creator
- Pomponi, Shirley A.
- Date Issued
- 2001
- PURL
- http://purl.flvc.org/fau/fd/FA00007240
- Subject Headings
- Marine natural products, Marine pharmacology, Drug development
- Format
- Document (PDF)
- Title
- Conclusions and perspectives for the future.
- Creator
- O'Shea, T. J., Bossart, Gregory D., Fournier, M., Vos, Joseph G., Harbor Branch Oceanographic Institute
- Date Issued
- 2003
- PURL
- http://purl.flvc.org/FCLA/DT/3352939
- Subject Headings
- Marine mammals, Toxicology--Research, Marine toxins
- Format
- Document (PDF)
- Title
- Natural Product Studies of Marine Organisms from the Western Atlantic.
- Creator
- Hall, Andrew, West, Lyndon, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
The projects described in this dissertation are focused on compounds derived from marine organisms collected from the western Atlantic marine environment. Chapter 1 provides an introduction to the study of natural products chemistry, marine natural products, and overview of the research undertaken from natural product chemists. Chapter 2 describes the isolation and structure elucidation of a series of rare diterpenoids from the gorgonian Briareum asbestinum, together with their conformational...
Show moreThe projects described in this dissertation are focused on compounds derived from marine organisms collected from the western Atlantic marine environment. Chapter 1 provides an introduction to the study of natural products chemistry, marine natural products, and overview of the research undertaken from natural product chemists. Chapter 2 describes the isolation and structure elucidation of a series of rare diterpenoids from the gorgonian Briareum asbestinum, together with their conformational analysis and biosynthetic interconversions. These rare diterpenes from Briareum asbestinum are linked by an unusual transannular oxa-6π electrocyclization which is described in detail and this work demonstates the biomimetic hemisynthesis of briareolate esters L (19) to B (22) achieved via an intermediary, briareolate ester G (2), through a controlled set of photoinduced isomerizations and a unique photochromic transannular oxa-6π electrocyclization. This work focuses largely on the mechanistic understanding of the photochemical production of these briarane diterpenoids and illustrates a unique UVA/UVC, photochromic switch which induces a transannular oxa- 6π electrocyclization. Chapter 3 describes the assay-guided isolation of marine antioxidants. This chapter focuses on the screening of marine organism extracts using the Ferric Reducing Antioxidant Power (FRAP) assay for antioxidant activity guided isolation of marine natural products. The chapter concludes with the activity guided isolation and structural elucidation of 1-O-palmitoyl-2-O-myristoyl-3-O-(6-sulfo-α-D-quinovopyranosyl)- glycerol (40) to show direct antioxidant potential through FRAP analysis. Chapter 4 describes the isolation, structural elucidation and pharmacological evaluation of the novel secondary metabolites iso-PsA(45), Iso-PsC (46), iso-PsD (47) as well as known Pseudopterosins A(41), B(42), C(43), D(44), K(48), K2’OAc(49), K2’OAc(50). These secondary metabolites were evaluated for both cytotoxicity. The chapter concludes with the screening of these compounds as αβ-amyloid fibril modulators utilizing atomic force microcopy (AFM).
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00004666
- Subject Headings
- Marine natural products., Diterpenes., Marine metabolites
- Format
- Document (PDF)
