Current Search: Keating, Patricia (x)
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- Title
- Potential estuarine water quality improvement via marine invertebrate bioremediation.
- Creator
- Draughon, Lisa D., Scarpa, John, Keating, Patricia, Hartmann, James X.
- Date Issued
- 2008
- PURL
- http://purl.flvc.org/fau/fd/FA00007399
- Subject Headings
- Estuaries, Water quality, Marine invertebrates, Bioremediation
- Format
- Document (PDF)
- Title
- Quantification and Analysis of Cancer-Cell Derived Exosomes.
- Creator
- Urdaneta, Alexandra, Iragavarapu-Charyulu, Vijaya, Keating, Patricia
- Abstract/Description
-
Over 90% of cancer-related deaths are due to metastasis. Tumor-cell derived extracellular vesicles or exosomes are thought to contribute to metastasis. However, there is no standardized method to isolate exosomes. We hypothesize that polymer-based kits, in particular ExoQuick-TC (EQTC), may be better for isolating exosomes when compared to ultracentrifugation. In this study, we used three different methods to isolate exosomes from 4T1 murine mammary tumor cells. Samples isolated through...
Show moreOver 90% of cancer-related deaths are due to metastasis. Tumor-cell derived extracellular vesicles or exosomes are thought to contribute to metastasis. However, there is no standardized method to isolate exosomes. We hypothesize that polymer-based kits, in particular ExoQuick-TC (EQTC), may be better for isolating exosomes when compared to ultracentrifugation. In this study, we used three different methods to isolate exosomes from 4T1 murine mammary tumor cells. Samples isolated through ultracentrifugation (UC), EQTC, and Total Exosome Isolation Reagent (TEIR) were analyzed to assess quantity and quality of exosomes by nanoparticle tracking analysis (NTA) and flow cytometry (FC). Using NTA, we found that each method yielded samples with varying average concentration and particle size. FC analysis revealed UC to be the most effective method in yielding a high number of verified exosomes. Standardizing the isolation method and assessment will help in determining the role of exosomes in cancer metastasis.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FAU_SR00000055
- Subject Headings
- College students --Research --United States.
- Format
- Document (PDF)
- Title
- Hypoxia Inducible Factor-Alpha Promotes Expression of Pro-tumorigenic Semaphorin7A in Mammary Tumor Cells.
- Creator
- Karram, Joseph P., Garcia-Areas, Ramon A., Libreros, Stephania, Graduate College, Castro, C., Keating, Patricia, Iragavarapu-Charyulu, Vijaya
- Abstract/Description
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It is estimated that one in eight women will be diagnosed with breast cancer. Developing an understanding of the tumor microenvironment is critical for developing treatments for breast cancer patients. It has been well established that hypoxia, or a lack of oxygen, is fundamental in creating a microenvironment that enables metastasis via eliciting angiogenic processes. Poorly differentiated blood vessels can fashion an oxygen-deprived microenvironment that triggers the expression of...
Show moreIt is estimated that one in eight women will be diagnosed with breast cancer. Developing an understanding of the tumor microenvironment is critical for developing treatments for breast cancer patients. It has been well established that hypoxia, or a lack of oxygen, is fundamental in creating a microenvironment that enables metastasis via eliciting angiogenic processes. Poorly differentiated blood vessels can fashion an oxygen-deprived microenvironment that triggers the expression of transcription factor Hypoxia Inducible Factor alpha HIF-1alpha that in turn can up regulate genes mediating a protumor effect. Our laboratory has discovered that mammary tumors express Semaphorin7a SEMA7A, a HIF-1alpha inducible protein. This study’s objective is to delineate the mechanism for hypoxia induction in mammary cells. Cobalt Chloride CoCl2 was used to mimic hypoxia in mammary tumor cell cultures. Flow cytometry was used to determine HIF-1alpha activity in the mammary cells. Benign EpH4 mammary cells expressing low SEMA7A greatly increased its expression after response to hypoxic stimuli as determined by qPCR and SEMA7A-specific ELISA. Pretreatment of EpH4 cells with a HIF-1alpha inhibitor Chemotin blocked induction of SEMA7A. Paradoxically, CoCl2 did not raise expression in the highly metastatic 4T1 cells which experience high levels of SEMA7A. Treatment of 4T1 cells with Chemotin under normoxic conditions inhibit HIF-1alpha activity and decrease SEMA7A levels. Determining the role of SEMA7A in the hypoxia axis could further elucidate novel pathways in breast cancer, suggesting that malignant tumor cells can utilize HIF-1alpha in a hypoxic independent manner.
Show less - Date Issued
- 2015
- PURL
- http://purl.flvc.org/fau/fd/FA00005887
- Format
- Document (PDF)
- Title
- Exploring the role of CHI3L1 in “pre-metastatic” lungs of mammary tumor-bearing mice.
- Creator
- Libreros, Stephania, Garcia-Areas, Ramon A., Keating, Patricia, Carrio, Roberto, Iragavarapu-Charyulu, Vijaya
- Date Issued
- 2013
- PURL
- http://purl.flvc.org/fau/fd/FAUIR000157
- Format
- Citation
- Title
- Semaphorin7A promotes tumor growth and exerts a pro-angiogenic effect in macrophages of mammary tumor-bearing mice.
- Creator
- Garcia-Areas, Ramon A., Libreros, Stephania, Amat, Samantha, Keating, Patricia, Carrio, Roberto, Robinson, Phillip, Blieden, Clifford, Iragavarapu-Charyulu, Vijaya
- Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FAUIR000158
- Format
- Citation
- Title
- Systemic Lupus Erythematosus: A.Studies on an ldiotype Specific Dendritic Cell Vaccine. B.Association of Lupus Calcinosis with Calcifying Nanoparticles.
- Creator
- Keating, Patricia, Florida Atlantic University, Hartmann, James X., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Systemic lupus erythrematosus (SLE) is an autoimmune disease characterized by the production of anti-DNA antibodies. The primary goal of this study was to activate T cells with specificity toward lupus B cells presenting anti-DNA antibody idiotopes on their surface. Monocyte derived dendritic cells were obtained from peripheral blood of healthy donors and lupus patients. Affinity purified anti-DNA antibodies were obtained from lupus patients' plasmas. The efficacy of different carrier...
Show moreSystemic lupus erythrematosus (SLE) is an autoimmune disease characterized by the production of anti-DNA antibodies. The primary goal of this study was to activate T cells with specificity toward lupus B cells presenting anti-DNA antibody idiotopes on their surface. Monocyte derived dendritic cells were obtained from peripheral blood of healthy donors and lupus patients. Affinity purified anti-DNA antibodies were obtained from lupus patients' plasmas. The efficacy of different carrier proteins, conjugated to lgG, was evaluated, and KLH found to be the most efficient for antigen uptake. The cognate dendritic cells were evaluated for their capacity to activate autologous T cells, and generate a Th1 mediated response which was evaluated by proliferation assays and interferony secretion. During the vaccine study a patient presented with panniculitis, CREST syndrome and a calcinotic exudate. A secondary goal of my study was to analyze this exudate. Calcifying nanoparticles were isolated in lymphocyte culture medium. They were characterized by Von Kassa staining for hydroxyapatite, solubilization by the calcium chelating agent EDT A, and light and scanning electron microscopy. A novel method was developed, using a specific monoclonal antibody, to analyze the calcifying nanoparticles. This method allowed for an approximate quantification of the particles. These particles increased in numbers when incubated for different time periods, and their numbers were decreased when incubated in the presence of minocyclin. Concomitantly, the panniculi in the patient underwent remission with long term antibiotic therapy. CNPs were also obtained from fetal bovine serum and human plasma samples from both lupus patients and healthy donors. Peripheral blood mononuclear cells from healthy donors and lupus patients were analyzed in vitro for their reactivity when incubated in the presence of a biofilm, generated by the calcifying nanoparticles. Viability, proliferation, and co-stimulatory marker up-regulation were determined in the presence or absence of the particles. Osteopontin was found highly expressed in the supernatants of the cells grown with CNPs. Microarray of the mononuclear cells of a healthy donor and a lupus patient incubated in the presence or absence of CNPs was performed, and the results coincided with those determined in vitro.
Show less - Date Issued
- 2007
- PURL
- http://purl.flvc.org/fau/fd/FA00000865
- Subject Headings
- Systemic lupus erythematosus--Molecular aspects, Systemic lupus erythematosus--Immunological aspects, Cell surface antigens, Autoimmune diseases--Research, Monoclonal antibodies--Diagnostic use
- Format
- Document (PDF)