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Title: Effects of GUS Gene Integration in Tobacco Plants.
Creator: Cruz, Laura, Zhang, Xing-Hai
Abstract/Description: The β-glucuronidase (GUS) gene was isolated in 1986 from the bacterium Escherichia coli. Since then it has been widely used as a reporter gene in genetically modified organisms serving to study gene expression and tissue specificity of different promoter sequences. We have introduced the GUS gene into tobacco plants through Agrobacterium-mediated genomic transformation. The plants that were confirmed to be expressing the GUS gene were grown to propagate a new (T1) generation. The T1 plants...
Show moreThe β-glucuronidase (GUS) gene was isolated in 1986 from the bacterium Escherichia coli. Since then it has been widely used as a reporter gene in genetically modified organisms serving to study gene expression and tissue specificity of different promoter sequences. We have introduced the GUS gene into tobacco plants through Agrobacterium-mediated genomic transformation. The plants that were confirmed to be expressing the GUS gene were grown to propagate a new (T1) generation. The T1 plants were analyzed for tissue specificity of GUS expression. The results to date seem to indicate that there is some variation in GUS expression between plant lines. The mechanisms of GUS gene integration in the plant genome as well as the possible effects it can have on a plant’s genomic structure are assessed.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FA0005009
Subject Headings: College students --Research --United States.
Format: Document (PDF)

Title: Enhancement of nutrional value in tomato plants.
Creator: Hill, William, Zhang, Xing-Hai
Date Issued: 2013-04-05
PURL: http://purl.flvc.org/fcla/dt/3361091
Subject Headings: Tomatoes, Tomatoes--Nutrition, Lysine, Plant genetic engineering, Genetically modified foods
Format: Document (PDF)

Title: Diagnosis of Citrus Greening Disease by qPCR Analysis.
Creator: Rocha, Fernando, Zhang, Xing-Hai
Abstract/Description: Citrus greening, also known as Huanglongbing disease, is a phloem restrictive disease that affects orange as well as other citrus trees. The disease is caused by the gram negative bacteria Candidatus Liberibacter asiaticus. The bacteria is transmitted by the Asian psyllid, Diaphorina citri. The bacteria causes the tree to produce small and bitter oranges, the roots shrink and the leaves molt. There is currently no cure for this disease. The best way to manage citrus greening is by removing...
Show moreCitrus greening, also known as Huanglongbing disease, is a phloem restrictive disease that affects orange as well as other citrus trees. The disease is caused by the gram negative bacteria Candidatus Liberibacter asiaticus. The bacteria is transmitted by the Asian psyllid, Diaphorina citri. The bacteria causes the tree to produce small and bitter oranges, the roots shrink and the leaves molt. There is currently no cure for this disease. The best way to manage citrus greening is by removing infected trees, implementing healthy planting material and controlling the psyllid population. Quantitative real time PCR (qPCR) was used to verify whether or not a given orange tree had citrus greening disease. DNA was extracted from leaves from eight trees. A qPCR analysis was performed using a primer with the bacteria DNA. Three trees were successfully diagnosed with citrus greening using this method.
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Date Issued: 2018
PURL: http://purl.flvc.org/fau/fd/FAU_SR00000051
Subject Headings: College students --Research --United States.
Format: Document (PDF)

Title: Regulatory Pattern of PUN Promoter for Gene Expression.
Creator: Velez, Stephanie, Kirke, Justin, Zhang, Xing-Hai, Office of Undergraduate Research and Inquiry
Abstract/Description: The purpose of this research was to analyze the regulatory pattern of the PUN promoter in the expression of a marker gene, β-glucoronidase (GUS), within regenerated tobacco plants. The genes for neomycin phosphotransferase (NPT II) and GUS were included in the coding region of the Ti plasmid construct. The NPTII gene drove antibiotic resistance and was used to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical staining and genetic...
Show moreThe purpose of this research was to analyze the regulatory pattern of the PUN promoter in the expression of a marker gene, β-glucoronidase (GUS), within regenerated tobacco plants. The genes for neomycin phosphotransferase (NPT II) and GUS were included in the coding region of the Ti plasmid construct. The NPTII gene drove antibiotic resistance and was used to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical staining and genetic assays rendered putative transgenic lines that were cultivated for further assessment of progeny. First generation histochemical analysis of 14-day tissue formation resulted in no levels of expression for the GUS gene, which demonstrated that the flower-specific PUN promoter was not active in the leaf tissue. Further testing of gene activity throughout all stages of tissue formation for the first generation lines is required in order to assess regulatory pattern of the PUN promoter.
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Date Issued: 2016
PURL: http://purl.flvc.org/fau/fd/FA00005606
Subject Headings: College students --Research --United States.
Format: Document (PDF)

Title: Genetic Engineering of Tomato Plants Expressing β-Glucuronidasethrough Agrobacterium-mediated Transformation.
Creator: Justs, Adriana, Kaplan, Noah, Zhang, Xing-Hai, Office of Undergraduate Research and Inquiry
Abstract/Description: Advancements in biotechnology have allowed us to study genetics and plant physiology by engineering transgenic plants. For our research we transformed Micro-Tom, a tomato variety developed for use in genetic research, using Agrobacterium mediated transformation. Within a time span of fourteen weeks, we inserted two distinct plasmid constructs (pCAMBIA2301 and E1492). Plants have the unique ability to regenerate their tissue and we took advantage of this ability to regenerate the transgenic...
Show moreAdvancements in biotechnology have allowed us to study genetics and plant physiology by engineering transgenic plants. For our research we transformed Micro-Tom, a tomato variety developed for use in genetic research, using Agrobacterium mediated transformation. Within a time span of fourteen weeks, we inserted two distinct plasmid constructs (pCAMBIA2301 and E1492). Plants have the unique ability to regenerate their tissue and we took advantage of this ability to regenerate the transgenic plants with antibiotic selection. Approximately one third of the explants endured the infection process and fourteen of these survived in the presence of kanamycin. By the end of the fourteenth week, eleven out of our fourteen plantlets had fully developed roots but only four survived to maturity. After verification with PCR and qPCR, we found that we generated two transgenic plants. Here we describe all the methods and techniques used to achieve these compelling results.could be the potential cause of this neurodegenerative disease, will help elucidate the role of this amino acid in ALS.
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Date Issued: 2016
PURL: http://purl.flvc.org/fau/fd/FA00005578
Subject Headings: College students --Research --United States.
Format: Document (PDF)

Title: Characterization of Methionine Sulfoxide Reductases A and Bs from Tobacco Plant.
Creator: Ding, Di, Zhang, Xing-Hai, Florida Atlantic University
Abstract/Description: One methionine sulfoxide reductase A (TMSRA) and two methionine sulfoxide reductase Bs (TMSRB 1 and TMSRB2) were isolated from tobacco plants. TMSRA showed specificity for the reduction of Met-(S)-SO and both TMSRBs were specific for the reduction of Met-(R)-SO. TMSRA was the cytosolic form and both TMSRBs were plastid forms based on sequence comparison and expression tests. TMSRA and TMSRB2 could use either thioredoxin (TRX) or dithiothreitol (DTT) as reducing system, while TMSRB 1 showed...
Show moreOne methionine sulfoxide reductase A (TMSRA) and two methionine sulfoxide reductase Bs (TMSRB 1 and TMSRB2) were isolated from tobacco plants. TMSRA showed specificity for the reduction of Met-(S)-SO and both TMSRBs were specific for the reduction of Met-(R)-SO. TMSRA was the cytosolic form and both TMSRBs were plastid forms based on sequence comparison and expression tests. TMSRA and TMSRB2 could use either thioredoxin (TRX) or dithiothreitol (DTT) as reducing system, while TMSRB 1 showed little activity with TRX but much more activity with DTT, which was similar to the mitochondrial MSRB2 from mammals. Ferredoxin (FD) is not the reducing system for Msrs, but might reflect the redox status in the cell and control the activity of Msrs indirectly.
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Date Issued: 2006
PURL: http://purl.flvc.org/fau/fd/FA00000746
Subject Headings: Proteins--Chemical modification, Genetic regulation, Plant genetic engineering, Antioxidants
Format: Document (PDF)

Title: Molecular Characterization of a Nicotiana tabacum Chloroplast Mutant and in vitro Regeneration of a Cattail, Typha domingensis, from Embryonic Callus.
Creator: Tapia, Claudius E., Zhang, Xing-Hai, Florida Atlantic University
Abstract/Description: The thesis consists of two chapters, and within each chapter is a different set of methods and techniques that will be useful to me in future research endeavors. The first is the transformation of tobacco chloroplast and the analysis of a resultant chloroplast mutant, and the second is the establishment of a regeneration system for the aquatic plant Typha domingensis, commonly known as cattail. The unifying theme is plant transformation. The establishment of a regeneration system for a...
Show moreThe thesis consists of two chapters, and within each chapter is a different set of methods and techniques that will be useful to me in future research endeavors. The first is the transformation of tobacco chloroplast and the analysis of a resultant chloroplast mutant, and the second is the establishment of a regeneration system for the aquatic plant Typha domingensis, commonly known as cattail. The unifying theme is plant transformation. The establishment of a regeneration system for a potentially beneficial plant is useful for future transformations and the actual transformation and analysis of mutants is useful for the characterization of transformants. The chloroplast transformation was unsuccessful and analysis of the mutation demonstrated it as a null mutation under normal growth conditions. Cattail seeds were induced to form calli, then induced to regenerate into normal plants.
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Date Issued: 2007
PURL: http://purl.flvc.org/fau/fd/FA00000839
Subject Headings: Plant cell culture, Plant biotechnology, Typha
Format: Document (PDF)

Title: Genetic variation amongst different populations of Typha domingensis and Typha latifolia (cattails) in the Florida Everglades.
Creator: Tapia, Manuel N., Florida Atlantic University, Zhang, Xing-Hai
Abstract/Description: This thesis is intended to explore the genetic variation between cattail species (Typha spp.), within T. domingensis in different locations, anthropoegenic conditions, and possibly discover a hybrid in the Florida Everglades. Typha domingensis is the dominant cattail species in the Everglades, while Typha latifolia a less common species is also present. Five nuclear and chloroplast protein encoding genes from around 20 samples of cattail plants were collected randomly in the Water...
Show moreThis thesis is intended to explore the genetic variation between cattail species (Typha spp.), within T. domingensis in different locations, anthropoegenic conditions, and possibly discover a hybrid in the Florida Everglades. Typha domingensis is the dominant cattail species in the Everglades, while Typha latifolia a less common species is also present. Five nuclear and chloroplast protein encoding genes from around 20 samples of cattail plants were collected randomly in the Water Conservation Areas of the Everglades Protection Act, cloned and sequenced. The results of sequencing showed differences between the two species studied, using an insertion within an intron of the Type 2 Metallothionein-like protein gene as a marker to differentiate between the two species. A high degree of nucleotide polymorphisms interspecifically was revealed. Species identification based on morphology is not always reliable that is why our marker must be utilized to confirm the identity of a plant.
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Date Issued: 2006
PURL: http://purl.flvc.org/fcla/dt/13415
Subject Headings: Everglades (Fla ), Typha--Florida--Everglades, Wetland plants--Florida--Everglades, Habitat (Ecology)--Florida--Everglades
Format: Document (PDF)

Title: Analysis of SLKED gene expression in CRISPR/Cas9-mediated gene knockouts in Tomato (Micro-Tom).
Creator: Vichyavichien, Paveena, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Clustered regularly interspaced short palindromic repeats (CRISPR/CRISPR-associated (Cas) protein system, CRISPR/Cas9, uses single-guide RNA to guide Cas9 to the target site for genome editing. In this study, the CRISPR/Cas9 system was used to knockout KED in tomato (Solanum lycopersicum). KED was first identified while screening the wounded tobacco (Nicotiana tabacum) leaves. We found that alignment of the protein sequence of SlKED (Solanum lycopersicum KED) and NtKED (Nicotiana tabacum KED)...
Show moreClustered regularly interspaced short palindromic repeats (CRISPR/CRISPR-associated (Cas) protein system, CRISPR/Cas9, uses single-guide RNA to guide Cas9 to the target site for genome editing. In this study, the CRISPR/Cas9 system was used to knockout KED in tomato (Solanum lycopersicum). KED was first identified while screening the wounded tobacco (Nicotiana tabacum) leaves. We found that alignment of the protein sequence of SlKED (Solanum lycopersicum KED) and NtKED (Nicotiana tabacum KED) showed 55.1% identity. To investigate, we generated SlKED knockout tomato plants with a single base pair deletion, a five base pair deletion and a three base pair deletion with a single base pair insertion. We performed wounding assays and analyzed gene expression and found that the wounded SlKED knockout plant showed no gene induction. Furthermore, the biological assay results revealed that the tobacco hornworm (Manduca sexta) gained more mass when fed on the SlKED knockout plant. Our studies show that the KED gene plays a role in wound-induced mechanism and suggested it may involve in the plant defense system against biological stress and insect feeding.
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Date Issued: 2019
PURL: http://purl.flvc.org/fau/fd/FA00013275
Subject Headings: Genome editing, Gene expression, CRISPR/Cas9, CRISPR-associated protein 9
Format: Document (PDF)

Title: Analysis of Tardigrade Damage Suppressor Protein (Dsup) Expressed in Tobacco.
Creator: Kirke, Justin, Zhang, Xing-Hai, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: DNA damage is one of the most harmful stress inducers in living organisms. Studies have shown that exposure to high doses of various types of radiation cause DNA sequence changes (mutation) and disturb protein synthesis, hormone balance, leaf gas exchange and enzyme activity. Recent discovery of a protein called Damage Suppressor Protein (Dsup), found in the tardigrade species Ramazzotius varieornatus, has shown to reduce the effects of radiation damage in human cell lines. We have generated...
Show moreDNA damage is one of the most harmful stress inducers in living organisms. Studies have shown that exposure to high doses of various types of radiation cause DNA sequence changes (mutation) and disturb protein synthesis, hormone balance, leaf gas exchange and enzyme activity. Recent discovery of a protein called Damage Suppressor Protein (Dsup), found in the tardigrade species Ramazzotius varieornatus, has shown to reduce the effects of radiation damage in human cell lines. We have generated multiple lines of tobacco plants expressing the Dsup gene and preformed numerous tests to show viability and response of these transgenic plants when exposed to mutagenic chemicals, UV radiation and ionizing radiation. We have also investigated Dsup function in association to DNA damage and repair in plants by analyzing the expression of related genes using RT-qPCR. We have also analyzed DNA damage from X-ray and UV treatments using an Alkaline Comet Assay. This project has the potential to help generate plants that are tolerant to more extreme stress environments, particularly DNA damage and mutation, unshielded by our atmosphere. The possibility of growing plants accompanying human space travel and extraterrestrial colonization inspires our imagination. Extremotolerant tardigrade genes such as Dsup may be a valuable avenue in helping to cultivate crops in these future endeavors.
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Date Issued: 2019
PURL: http://purl.flvc.org/fau/fd/FA00013384
Subject Headings: DNA damage, DNA Damage--radiation effects, Tardigrada, DNA Repair, Transgenic plants
Format: Document (PDF)

Title: Expression of a Dehydrin from the Polar Plant Cerastium arcticum in Transgenic Tobacco.
Creator: Hill, William, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Water scarcity induced by drought, temperature, and salinity has plagued agricultural sustainability in recent years with unprecedented revenue losses, raising concerns for worldwide food security. Recent studies have revealed unique botanical response mechanisms to combat water related stress, namely the expression of proteins known as the dehydrins. Dehydrin proteins have been shown to serve various intracellular protective functions. The gene for a SK5 type dehydrin from the arctic plant...
Show moreWater scarcity induced by drought, temperature, and salinity has plagued agricultural sustainability in recent years with unprecedented revenue losses, raising concerns for worldwide food security. Recent studies have revealed unique botanical response mechanisms to combat water related stress, namely the expression of proteins known as the dehydrins. Dehydrin proteins have been shown to serve various intracellular protective functions. The gene for a SK5 type dehydrin from the arctic plant Cerastium arcticum (CaDHN) was introduced into tobacco plants and water deficit tolerance was evaluated. Plants overexpressing CaDHN displayed improved tolerance to salt stress, but no improvement was observed under drought stress.
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Date Issued: 2015
PURL: http://purl.flvc.org/fau/fd/FA00004505, http://purl.flvc.org/fau/fd/FA00004505
Subject Headings: Cerastium., Plant bioitechnology., Plants, Effect of stress on., Crops, Effect of stress on., Plant gene expression., Plant proteins., Recombinant proteins.
Format: Document (PDF)

Title: Nitrate Use Efficiency In Tobacco Plants Constitutively Expressing A Maize Nitrate Transporter ZmNRT2.1.
Creator: Cruz, Jessica, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: The NRT2 (high affinity nitrate transporter 2) family is a part of the iHATS (inducible high affinity system) that studies have shown is responsible for the influx of nitrate into the plant cell after provision of nitrate. The ZmNRT2.1 from Zea mays was constitutively expressed in Nicotiana tabacum. To assess how over-expression of this foreign NRT2.1 affects nitrate influx by plants, nitrate content in leaf and root tissue, gene expression, and vegetal growth were analyzed in media with...
Show moreThe NRT2 (high affinity nitrate transporter 2) family is a part of the iHATS (inducible high affinity system) that studies have shown is responsible for the influx of nitrate into the plant cell after provision of nitrate. The ZmNRT2.1 from Zea mays was constitutively expressed in Nicotiana tabacum. To assess how over-expression of this foreign NRT2.1 affects nitrate influx by plants, nitrate content in leaf and root tissue, gene expression, and vegetal growth were analyzed in media with deficient or high nitrate concentrations (0.1, 1, or 10 mM). Compared to wild type plants: the transgenic lines had a significantly larger fresh weight in all nitrate conditions; primary root length was significantly longer in the 0.1 and 1 mM nitrate conditions; both the fresh weight and the primary root length were significantly higher when 50 mM NaCl was applied as a stress factor to medias containing 0.1 and 10 mM nitrate.
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Date Issued: 2015
PURL: http://purl.flvc.org/fau/fd/FA00004492, http://purl.flvc.org/fau/fd/FA00004492
Subject Headings: Nitrogen--Fixation., Nitrogen-fixing plants--Metabolism., Crops and nitrogen., Field crops--Genetic engineering., Plants--Effect of nitrogen on., Soil microbiology.
Format: Document (PDF)

Title: The expression and analysis of a lysine-rich wound-response protein in tomato plants.
Creator: Kaplan, Noah, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Understanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum...
Show moreUnderstanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum lycopersicum) that we named SlKED. We have analyzed gene expression pattern of SlKED through tomato growth and development and in response to wounding as well as hormonal and inhibitor treatments. We found that the plant hormone ethylene played a major role in the expression of SlKED. To further identify evidence for physiological and transductional functions of KED and SlKED, the tobacco KED gene was introduced to tomato and overexpressed by the fruit tissue-active PUN1 promoter from pepper (Capsicum annuum,). The expression of this gene was compared to the expression of the native SlKED gene and other known wound response genes in both the wild-type and transgenic tomato plants. The upregulation of the native SlKED gene by wounding was significantly muted in the tobacco KED-expressing transgenic plants. The expression of other genes known to be associated with wound response transduction pathways was also altered. Our studies implicate the KED gene in defense mechanisms for mechanical stress in tomato plants.
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Date Issued: 2016
PURL: http://purl.flvc.org/fau/fd/FA00004773, http://purl.flvc.org/fau/fd/FA00004773
Subject Headings: Wound healing., Wounds and injuries--Genetic aspects., Plant gene expression., Plant genetic regulation., Nanostructured materials--Physiological effect., Biomedical engineering.
Format: Document (PDF)

Title: Targeted Gene Knock-out Via Promoter Tagging in Tomato (Lycopersicon Esculentum) and Tobacco (Nicotiana Tabacum).
Creator: Kazy, Alia, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Many different ways to create mutants have been established. This research demonstrates yet another variation of the promoter tagging technique that allows for a single step selection of the putative transgenic plants that have a mutation in constitutively expressed genes. While tomato transformants have not yet been convincingly confirmed, tobacco transformation resulted in seven transgenic lines showing resistance to high concentrations of kanamycin. Two transgenic lines were further...
Show moreMany different ways to create mutants have been established. This research demonstrates yet another variation of the promoter tagging technique that allows for a single step selection of the putative transgenic plants that have a mutation in constitutively expressed genes. While tomato transformants have not yet been convincingly confirmed, tobacco transformation resulted in seven transgenic lines showing resistance to high concentrations of kanamycin. Two transgenic lines were further investigated and three putative promoters isolated. Transient expression analysis of leaves transformed by particle bombardment with vectors carrying beta-glucuronidase gene driven by these putative promoters suggests two of them to be functional. Further investigation is needed to confirm the expression in the stably transformed plants as well as cloning of the genes downstream of the functional promoters and research of their functions.
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Date Issued: 2007
PURL: http://purl.flvc.org/fau/fd/FA00000779
Subject Headings: Gene mapping--Methodology, Gene mapping--Data processing, Gene expression, Microbial genetics, Computational biology
Format: Document (PDF)

Title: Analysis of KED-mediated wound response to biotic stress and mechanical damage in tomato plants (Solanum lycopersicum).
Creator: Nifakos, Nicholas, Zhang, Xing-Hai, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Lysine-rich KED was previously identified from wounded tobacco (Nicotiana tabacum) leaves before the alignment of protein sequences between NtKED (Nicotiana tabacum KED) and SlKED (Solanum lycopersicum KED) were discovered to display 55.1% identity. Using previously generated SlKED knockout plants by CRISPR/Cas9, we performed biological assays, to investigate the role of KED in wound response to biotic and abiotic stress. Previous studies implied that the KED gene functions as a role in the...
Show moreLysine-rich KED was previously identified from wounded tobacco (Nicotiana tabacum) leaves before the alignment of protein sequences between NtKED (Nicotiana tabacum KED) and SlKED (Solanum lycopersicum KED) were discovered to display 55.1% identity. Using previously generated SlKED knockout plants by CRISPR/Cas9, we performed biological assays, to investigate the role of KED in wound response to biotic and abiotic stress. Previous studies implied that the KED gene functions as a role in the wound-induced mechanism, as well as suggested that it may also function in the plant defense system against biotic stress and insect herbivory. The results from bioassays using tobacco hornworm (Manduca sexta) have proven inconclusive thus far. Expression of KED is induced not only by mechanical wounding but also by touching such as brushing the leaves, indicating that this gene is sensitive to subtle environmental signal and may be involved in defense response against abiotic stress. To further investigate the KED gene’s role in the plant defense system, biological assays using both specialist and generalist herbivores, transcription analysis using various phytohormone mutant plants, and Evans blue cellular damage assays were performed. Our findings imply that the KED gene does not seem to have a long-term effect on insect herbivory but may have a shortterm anti-feeding effect against insect herbivores. Results from the Evans blue membrane damage assay indicate the KED gene may provide some benefit to mechanically damaged plants in a short-term period post-wounding of leaf tissues. Using the SlKED knockout as genetic tool, we conclude that this gene does not confer resistance to insect herbivores over a long-term but seems to provide a beneficial defense response in the short-term. Our membrane damage assay results also imply that this gene may be involved in membrane stabilization and repair of cellular damage after mechanical wounding.
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Date Issued: 2022
PURL: http://purl.flvc.org/fau/fd/FA00014001
Subject Headings: Plant gene expression, Solanum lycopersicum, Tomatoes--Effect of stress on
Format: Document (PDF)

Title: Analysis of Tobacco Resistance to Saline Conditions via Endogenous Expression of SeNN24 gene from the halophyte plant Salicornia europaea.
Creator: Wynter, Lij, Zhang, Xing-Hai, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Freshwater salinization and expanding desertification threaten global agriculture. Promise lies in salt resistance genes found in Salicornia europaea, a halophyte that thrives in high-salt conditions partly due to protein action. We focused one of its genes, SeNN24. It enhanced salt resistance in yeast and shows promise in improving crop resilience. Our research introduced SeNN24 into tobacco via agrobacterial transformation, testing the plants under salt and drought conditions. The...
Show moreFreshwater salinization and expanding desertification threaten global agriculture. Promise lies in salt resistance genes found in Salicornia europaea, a halophyte that thrives in high-salt conditions partly due to protein action. We focused one of its genes, SeNN24. It enhanced salt resistance in yeast and shows promise in improving crop resilience. Our research introduced SeNN24 into tobacco via agrobacterial transformation, testing the plants under salt and drought conditions. The transformed tobacco showed superior tolerance of up to 400mM NaCl and drought, maintaining health and even flowering under stress. This suggests that SeNN24 could potentially confer significant salt and drought resistance to vital crops, protecting them from environmental challenges and enhancing agricultural sustainability.
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Date Issued: 2024
PURL: http://purl.flvc.org/fau/fd/FA00014441
Subject Headings: Halophytes, Tobacco, Salinity, Botany
Format: Document (PDF)

Title: FAU Climate Change Initiative Priority Theme: Research, Engineering, and Adaption to a Change Climate.
Creator: Berry, Leonard, Koch, Marguerite, Center for Environmental Studies, Benscoter, Brian, Comas, Xavier, Devlin, Donna, Fadiman, Maria, Gerstein, E., Herzing, Denise L., Hindle, Tobin, Milton, Sarah L., Oleinik, Anton E., Proffitt, C. Edward, Restrepo, Jorge I., Root, Tara L., Wyneken, Jeanette, Xie, Zhixiao, Zhang, Xing-Hai, Esnard, Ann-Margaret, Mitsova, Diana, Murley, J., Vos, J., Escaleras, Monica, Mehallis, M., Shaw, Eric H., Hardman, Guillermo [John], Lambert, Julie, Thomas, G., Arockiasamy, Madasamy, Bloetscher, Frederick, Carvalho, G., Dhanak, Manhar R., Frisk, George V., Kaisar, Evangelos I., Kalva, Hari, Meeroff, Daniel E., Rodriguez, Jarice, Scarlatos, Panagiotis (Pete) D., Shankar, Ravi, Teegavarapu, Ramesh, Brown, Clifford T., McAfee, Francis, Widener, Patricia, Dalgleish, Fraser R., Hanisak, M. Dennis, McMulloch, S., O'Corry-Crowe, Gregory, Pomponi, Shirley A., Reed, John K., Scarpa, John, Voss, Joshua, Heimlich, Barry N., Alvarez, R., Jolley, J., Edwards, A., Charles E. Schmidt College of Science, Harbor Branch Oceanographic Institute, College of Business, Dorothy F. Schmidt College of Arts and Letters, College of Education, College of Engineering and Computer Science
Date Issued: 2010
PURL: http://purl.flvc.org/fau/fd/FA00003457
Format: Citation

FAU Theses and Dissertations (11)	+ -
FAU Undergraduate Student Research (5)	+ -
FAU College Collections (1)	+ -

College students --Research --United States. (4)	+ -
Gene expression (2)	+ -
Plant gene expression. (2)	+ -
Plant genetic engineering (2)	+ -
Antioxidants (1)	+ -

Biomedical engineering. (1)	+ -
Botany (1)	+ -
CRISPR-associated protein 9 (1)	+ -
CRISPR/Cas9 (1)	+ -
Cerastium. (1)	+ -
Computational biology (1)	+ -
Crops and nitrogen. (1)	+ -
Crops, Effect of stress on. (1)	+ -
DNA Damage--radiation effects (1)	+ -
DNA Repair (1)	+ -
DNA damage (1)	+ -
Everglades (Fla ) (1)	+ -
Field crops--Genetic engineering. (1)	+ -
Gene mapping--Data processing (1)	+ -
Gene mapping--Methodology (1)	+ -
Genetic regulation (1)	+ -
Genetically modified foods (1)	+ -
Genome editing (1)	+ -
Habitat (Ecology)--Florida--Everglades (1)	+ -
Halophytes (1)	+ -
Lysine (1)	+ -
Microbial genetics (1)	+ -
Nanostructured materials--Physiological effect. (1)	+ -
Nitrogen--Fixation. (1)	+ -
Nitrogen-fixing plants--Metabolism. (1)	+ -

Electronic Thesis or Dissertation (11)	+ -
poster (5)	+ -
report (1)	+ -

Zhang, Xing-Hai (17)	+ -
Florida Atlantic University (11)	+ -
Charles E. Schmidt College of Science (9)	+ -
Department of Biological Sciences (8)	+ -
Hill, William (2)	+ -

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