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EVIDENCE FOR THE EXISTENCE OF THE TRICARBOXYLIC ACID CYCLE AND GLYOXYLATE PATHWAY IN CERTAIN MARINE FILAMENTOUS FUNGI
Title
EVIDENCE FOR THE EXISTENCE OF THE TRICARBOXYLIC ACID CYCLE AND GLYOXYLATE PATHWAY IN CERTAIN MARINE FILAMENTOUS FUNGI.
Creator
VEMBU, DEVI T., Florida Atlantic University
Abstract/Description
Cellulolytic marine isolates, Culcitalna achraspora and Halosphaeria mediosetigera, were shaker-grown at 25 C in artificial seawater containing glucose, NH4NO3 , tris(hydroxymethyl) aminomethane and yeast extract, pH 7.5. Cultures were harvested in the late linear phase where whole cell endogenous respiration was maximal. Spectrophotometric determinations on acetone powder extracts revealed the presence of aconitase, NAD-linked isocitrate dehydrogenase, succinate dehydrogenase, fumarase,...
Show moreCellulolytic marine isolates, Culcitalna achraspora and Halosphaeria mediosetigera, were shaker-grown at 25 C in artificial seawater containing glucose, NH4NO3 , tris(hydroxymethyl) aminomethane and yeast extract, pH 7.5. Cultures were harvested in the late linear phase where whole cell endogenous respiration was maximal. Spectrophotometric determinations on acetone powder extracts revealed the presence of aconitase, NAD-linked isocitrate dehydrogenase, succinate dehydrogenase, fumarase, malate dehydrogenase and isocitratase. Culcitalna also showed NADP-linked isocitrate dehydrogenase activity. Manometric examinations of acetone powder and dialyzed sonic extracts were largely confirmatory. Oxidation of citric acid cycle intermediates by extracts were observed with Culcitalna for all but succinate with sonic extracts and for all but succinate and 2-oxoglutarate in acetone powder extracts. With Halosphaeria, neither extract could oxidize citrate or succinate, while acetone powder extracts were unable to degrade cis-aconitate and 2-oxoglutarate. Native mycelia were active only against malate. Attempts to permeate cell walls with ethyl esters of intermediates failed.
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Date Issued
1972
PURL
http://purl.flvc.org/fcla/dt/13478
Subject Headings
Marine fungi
Format
Document (PDF)