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(1 - 9 of 9)
- Title
- Organotin Polyethers as Biomaterials.
- Creator
- Carraher, Charles E., Roner, Michael R.
- Abstract/Description
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Organotin polyethers are easily synthesized employing interfacial polymerization systems involving the reaction of hydroxyl-containing Lewis bases and organotin halides. A wide variety of organotin-containing polymeric products have been synthesized including those derived from natural and synthetic polymers such as lignin, xylan, cellulose, dextran, and poly(vinyl alcohol). Others have been synthesized employing known drug diols such as dicumarol, DES, and dienestrol and a wide variety of...
Show moreOrganotin polyethers are easily synthesized employing interfacial polymerization systems involving the reaction of hydroxyl-containing Lewis bases and organotin halides. A wide variety of organotin-containing polymeric products have been synthesized including those derived from natural and synthetic polymers such as lignin, xylan, cellulose, dextran, and poly(vinyl alcohol). Others have been synthesized employing known drug diols such as dicumarol, DES, and dienestrol and a wide variety of synthetic diols. Included in these materials are the first water soluble organotin polymers. The organotin polyethers exhibit a wide range of biological activities. Some selectively inhibit a number of unwanted bacteria, including Staph. MRSA, and unwanted yeasts such as Candida albicans. Some also inhibit a variety of viruses including those responsible for herpes infections and smallpox. Others show good inhibition of a wide variety of cancer cell lines including cell lines associated with ovarian, colon, lung, prostrate, pancreatic and breast cancer. The synthesis, structural characterization, and biological characterization of these materials is described in this review.
Show less - Date Issued
- 2009-10-21
- PURL
- http://purl.flvc.org/fau/fd/FAUIR000010
- Format
- Citation
- Title
- Structural Consideration in Designing Organotin Polyethers to Arrest the Growth of Breast Cancer Cells In Vitro.
- Creator
- Carraher, Charles E., Roner, Michael R., Shahi, Kimberly, Barot, Girish
- Abstract/Description
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The ability to inhibit cancer is inherent in organotin materials yet the structural relationships that regulate/direct this activity remains unknown. We measured antitumor activity using a matched pair of cell lines MDA-MB-231 cells that are estrogen-independent, estrogen receptor negative and MCF-7 cells, a cell line that is estrogen receptor (ER) positive. Those polyethers that contained a O-phenyl unit were able to significantly inhibit the non-estrogen sensitive cell line but were much...
Show moreThe ability to inhibit cancer is inherent in organotin materials yet the structural relationships that regulate/direct this activity remains unknown. We measured antitumor activity using a matched pair of cell lines MDA-MB-231 cells that are estrogen-independent, estrogen receptor negative and MCF-7 cells, a cell line that is estrogen receptor (ER) positive. Those polyethers that contained a O-phenyl unit were able to significantly inhibit the non-estrogen sensitive cell line but were much less effective against the estrogen sensitive cell line; that is, the human breast cancer cell line MDA-MB-231 showed better test results for polymers derived from diols containing the O-phenyl moiety than the breast cancer cell line MCF-7, a well-characterized estrogen receptor positive control cell line. Those polyethers that did not contain the O-phenyl unit inhibited both cell lines approximately the same. The differential activity of the O-phenyl-containing polyethers is likely due to the estrogen-sensitive cells combining with some of the organotin polyethers minimizing their ability to inhibit cell growth.
Show less - Date Issued
- 2011-04-15
- PURL
- http://purl.flvc.org/fau/fd/FAUIR000009
- Format
- Citation
- Title
- Antiviral Activity of Metal-Containing Polymers—Organotin and Cisplatin-Like Polymers.
- Creator
- Roner, Michael R., Carraher, Charles E., Shahi, Kimberly, Barot, Girish
- Abstract/Description
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Polymers containing platinum and to a lesser extent tin, have repeatedly demonstrated antitumor activity in vitro and in vivo against a variety of cell and tumor types. The mechanisms responsible for the antitumor activity include inducing a delay in cell proliferation and sister chromatid exchanges blocking tumor growth. As most DNA and some RNA viruses require, and even induce, infected cells to initiate DNA replication and subsequent cell division, compounds with antitumor activity will...
Show morePolymers containing platinum and to a lesser extent tin, have repeatedly demonstrated antitumor activity in vitro and in vivo against a variety of cell and tumor types. The mechanisms responsible for the antitumor activity include inducing a delay in cell proliferation and sister chromatid exchanges blocking tumor growth. As most DNA and some RNA viruses require, and even induce, infected cells to initiate DNA replication and subsequent cell division, compounds with antitumor activity will very likely also possess antiviral activity. This article examines the use of metal-containing polymers as a novel class of antivirals.
Show less - Date Issued
- 2011-05-27
- PURL
- http://purl.flvc.org/fau/fd/FAUIR000008
- Format
- Citation
- Title
- Ability of Group IVB metallocene polyethers containing dienestrol to arrest the growth of selected cancer cell lines.
- Creator
- Roner, Michael R., Carraher, Charles E., Shahi, Kimberly, Ashida, Yuki, Barot, Girish
- Date Issued
- 2009-10-07
- PURL
- http://purl.flvc.org/fcla/dt/3325102
- Format
- Document (PDF)
- Title
- Differential cell killing of normal and transformed human lung fibroblasts by reovirus.
- Creator
- Loeffler, Jennifer L., Florida Atlantic University, Roner, Michael R.
- Abstract/Description
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When infected with reovirus ST3, strain Dearing, normal and transformed human lung fibroblasts exhibit differential sensitivities. Transformed cells (WI38 VA13 2RA) are destroyed within four days. In contrast, normal cells (WI38) maintain a productive infection for as long as two months. Attempts to examine the differences between these cells included the use of cDNA subtraction and a reovirus sigma 1 protein affinity chromatography column, both of which were hampered by technical...
Show moreWhen infected with reovirus ST3, strain Dearing, normal and transformed human lung fibroblasts exhibit differential sensitivities. Transformed cells (WI38 VA13 2RA) are destroyed within four days. In contrast, normal cells (WI38) maintain a productive infection for as long as two months. Attempts to examine the differences between these cells included the use of cDNA subtraction and a reovirus sigma 1 protein affinity chromatography column, both of which were hampered by technical difficulties. Two-dimensional gel electrophoresis revealed patterns of differential protein expression between infected and mock-infected normal and transformed cells. Visual comparison of Coomassie blue-stained gels revealed one protein which was present in uninfected normal cells but was missing in all other samples, as well as five proteins that were present in infected and mock-infected normal cells but were missing in both transformed cell samples. Autoradiography of 35S-labeled cell samples revealed an additional eleven proteins not seen with Coomassie staining. Further characterization of these proteins should uncover the mechanism of differential cell killing by reovirus.
Show less - Date Issued
- 2000
- PURL
- http://purl.flvc.org/fcla/dt/15776
- Subject Headings
- Reoviruses, Fibroblasts, Cell transformation
- Format
- Document (PDF)
- Title
- Resistant or persistent reovirus infections: Of normal human embryonic lung fibroblast WI-38 cells.
- Creator
- Eukitis, Martine Marie., Florida Atlantic University, Roner, Michael R.
- Abstract/Description
-
Reovirus is a common virus that usually affects children; this infection causes symptoms such as respiratory and or gastrointestinal aliments. Morbidity most often occurs in impoverished countries where supportive hospitalization is not available. In the U.S. and other established countries morbidity is not an issue. When WI-38 cells are infected with reovirus the infection is either resisted by the cells or a persistent latent infection occurs. In this study, gene expression was analyzed by...
Show moreReovirus is a common virus that usually affects children; this infection causes symptoms such as respiratory and or gastrointestinal aliments. Morbidity most often occurs in impoverished countries where supportive hospitalization is not available. In the U.S. and other established countries morbidity is not an issue. When WI-38 cells are infected with reovirus the infection is either resisted by the cells or a persistent latent infection occurs. In this study, gene expression was analyzed by comparing Reovirus-infected WI-38 cells with mock infected cells. P.R.O.M(TM) analysis was performed on RNA sent to Genka Biotechnology Inc. Bioinformatics was used to analyze the data. Reovirus infection of the WI-38 cells resulted in increased mRNA levels for a number of transcription regulatory genes, and possibly more significant, decreased mRNA levels for some very important regulatory genes. These changes may be responsible for establishing the antiviral replication environment observed in these normal cells.
Show less - Date Issued
- 2003
- PURL
- http://purl.flvc.org/fcla/dt/12991
- Subject Headings
- Reoviruses, Genetic regulation, Fibroblasts
- Format
- Document (PDF)
- Title
- Selective destruction of transformed cells by reovirus: Cellular targets and active reovirus proteins analysis.
- Creator
- Alter, Sarah., Florida Atlantic University, Roner, Michael R.
- Abstract/Description
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It is known that Reovirus selectively destroys transformed cells, but the complex mechanism is not completely understood at this time. In this study, gene expression was examined by comparing Reovirus-infected SV-40 transformed human embryonic fibroblasts, with mock-infected cells. Among the 40 genes shown to be altered, 38 genes were up regulated, and 2 genes were down regulated. Out of the 40 genes having differential gene expression, 8 were significantly over induced. 3 of these were DNA...
Show moreIt is known that Reovirus selectively destroys transformed cells, but the complex mechanism is not completely understood at this time. In this study, gene expression was examined by comparing Reovirus-infected SV-40 transformed human embryonic fibroblasts, with mock-infected cells. Among the 40 genes shown to be altered, 38 genes were up regulated, and 2 genes were down regulated. Out of the 40 genes having differential gene expression, 8 were significantly over induced. 3 of these were DNA binding transcription factors. Activation of transcription factors following Reovirus infection suggests that gene expression is essential in Reovirus induced transformed cell killing. Another 3 genes were found to be tumor suppressor proteins and oncogenes expressed downstream of the Ras pathway. The over expression of these was shown to induce apoptosis induced cell killing by Reovirus. Finally, 2 of the 8 significantly up regulated genes cause cell cycle progression inhibition. The cell cycle arrest then leads to cell death.
Show less - Date Issued
- 2002
- PURL
- http://purl.flvc.org/fcla/dt/12966
- Subject Headings
- Reoviruses, Gene expression
- Format
- Document (PDF)
- Title
- An investigation of the nonstructural proteins of mammalian reovirus serotype 3: Mu NS and sigma NS.
- Creator
- Van Vliet, Kim Marie, Florida Atlantic University, Roner, Michael R.
- Abstract/Description
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The reovirus nonstructural proteins muNS and sigmaNS are thought to play a role in assortment. To produce large quantities of the reovirus nonstructural proteins, two recombinant vaccinia virus systems were used, one for expression of muNS and the other for expression of muNS. The reovirus gene is under the control of the phage T7 RNA polymerase promoter. A recombinant vaccinia virus containing the gene for T7 RNA polymerase was added and protein expression was determined. Protein expression...
Show moreThe reovirus nonstructural proteins muNS and sigmaNS are thought to play a role in assortment. To produce large quantities of the reovirus nonstructural proteins, two recombinant vaccinia virus systems were used, one for expression of muNS and the other for expression of muNS. The reovirus gene is under the control of the phage T7 RNA polymerase promoter. A recombinant vaccinia virus containing the gene for T7 RNA polymerase was added and protein expression was determined. Protein expression was confirmed by infecting mouse fibroblast L929 cells, harvesting the cells and running an SDS-PAGE gel, followed by western blot, followed by Western-immunoperoxidase assay using a polyclonal antibody for the reovirus proteins, or by radioimmunoprecipitation followed by an SDS-PAGE gel. Purification of the recombinant proteins was accomplished by ammonium sulfate fractionation and column chromatography. The purified proteins will be utilized to further investigate the role of the reovirus nonstructural proteins in assortment.
Show less - Date Issued
- 2002
- PURL
- http://purl.flvc.org/fcla/dt/12930
- Subject Headings
- Reoviruses, RNA viruses
- Format
- Document (PDF)
- Title
- Identification and characterization of a newly discovered reovirus strain.
- Creator
- Jerusalmi, Alan, Florida Atlantic University, Roner, Michael R., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
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In this study, we report the identification and characterization of a new Reovirus strain isolated from the spinal fluid of a human patient. Unlike other reovirus serotypes that can be isolated from fecal matter, this strain was isolated from the spinal fluid of a patient; which suggests it may be a more pathogenic strain. The new isolate was compared to the three known strains of mammalian reoviruses. We have found that the virus is not a reassortment of the other three serotypes. This was...
Show moreIn this study, we report the identification and characterization of a new Reovirus strain isolated from the spinal fluid of a human patient. Unlike other reovirus serotypes that can be isolated from fecal matter, this strain was isolated from the spinal fluid of a patient; which suggests it may be a more pathogenic strain. The new isolate was compared to the three known strains of mammalian reoviruses. We have found that the virus is not a reassortment of the other three serotypes. This was confirmed by examination of the migration rates of the genomic dsRNA segments of all four viruses. The new isolate demonstrated measurable differences in viral protein molecular weights, when compared to the proteins of the three known serotypes. The molecular weight of the sigma I protein of the new isolate, was found to be more then 3Kd smaller then sigma 1 obtained from the other three serotypes. The largest difference in protein molecular weights was found with mu1C, which was about 7Kd smaller. The growth rate of the new virus was very similar to that of the other three serotypes. Some differences were found in select cell lines.
Show less - Date Issued
- 2000
- PURL
- http://purl.flvc.org/fcla/dt/12684
- Subject Headings
- Reoviruses, RNA viruses
- Format
- Document (PDF)
