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FAU 2016 3MT® Three Minute Thesis Championship - Douglas Holmes
Title
FAU 2016 3MT® Three Minute Thesis Championship - Douglas Holmes.
Creator
Holmes, Douglas, Graduate College
Abstract/Description
The 3MT® competition celebrates the exciting research conducted by graduate students. Developed by The University of Queensland (UQ), the exercise cultivates students’ academic, presentation, and research communication skills. The competition supports their capacity to effectively explain their research in three minutes, in language appropriate to a non-specialist audience. The first 3MT® competition was held at the University of Queensland in 2008 with 160 students competing. In 2009 and...
Show moreThe 3MT® competition celebrates the exciting research conducted by graduate students. Developed by The University of Queensland (UQ), the exercise cultivates students’ academic, presentation, and research communication skills. The competition supports their capacity to effectively explain their research in three minutes, in language appropriate to a non-specialist audience. The first 3MT® competition was held at the University of Queensland in 2008 with 160 students competing. In 2009 and 2010 the 3MT® competition was promoted to other Australian and New Zealand universities and enthusiasm for the concept grew. Since 2011, the popularity of the competition has increased and 3MT® competitions are now held in over 170 universities across more than 18 countries worldwide.
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Date Issued
2016
PURL
http://purl.flvc.org/fau/fd/FA00005380
Subject Headings
College students --Research --United States.
Format
Video file
Creation of a bacteria surrogate to accelerate research on Ebolavirus Zaire
Title
Creation of a bacteria surrogate to accelerate research on Ebolavirus Zaire.
Creator
Varghese, Reen, Holmes, Douglas, Coarsey, C., Singkorat, D., Office of Undergraduate Research and Inquiry
Abstract/Description
Ebola Viral Disease (EVD) is a devastating illness with high infectivity and mortality rates. The 2014 West African EVD outbreak was unprecedented in case numbers and fatalities, and has highlighted the need to develop rapid Point of Care detection devices. Progress in the diagnosis and treatment of highly virulent pathogens like the Ebola virus is often limited by the small number of labs adequately equipped to handle them. This study is one of the first to aim at developing a non-pathogenic...
Show moreEbola Viral Disease (EVD) is a devastating illness with high infectivity and mortality rates. The 2014 West African EVD outbreak was unprecedented in case numbers and fatalities, and has highlighted the need to develop rapid Point of Care detection devices. Progress in the diagnosis and treatment of highly virulent pathogens like the Ebola virus is often limited by the small number of labs adequately equipped to handle them. This study is one of the first to aim at developing a non-pathogenic bacterium surrogate, containing a stable EBV gene for subsequent detection studies. Our approach entailed the use of synthetic biology, to design a recombinant vector containing the Ebola virus glycoprotein (GP) gene. The synthetic gene was spliced into a E.coli pUC19 plasmid vector by ligation and subsequently transformed into competent E. coli by cloning techniques. This Ebola virus surrogate will assist in further Ebola diagnostic platform design and testing.
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Date Issued
2016
PURL
http://purl.flvc.org/fau/fd/FA00005605
Subject Headings
College students --Research --United States.
Format
Document (PDF)
Using Synthetic Biology to Create a Safe and Stable Ebola Surrogate for Effective Development of Detection and Therapy Platforms
Title
Using Synthetic Biology to Create a Safe and Stable Ebola Surrogate for Effective Development of Detection and Therapy Platforms.
Creator
Holmes, Douglas, Esiobu, Nwadiuto, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
Ebolavirus is responsible for a deadly hemorrhagic fever that has claimed thousands of lives in Africa and could become a global health threat. Because of the danger of infection, novel Ebola research is restricted to BSL-4 laboratories; this slows progress due to both the cost and expertise required to operate these laboratories. The development of a safe surrogate would speed research and reduce risk to researchers. Two highly conserved Ebola gene segments—from the glycoprotein and...
Show moreEbolavirus is responsible for a deadly hemorrhagic fever that has claimed thousands of lives in Africa and could become a global health threat. Because of the danger of infection, novel Ebola research is restricted to BSL-4 laboratories; this slows progress due to both the cost and expertise required to operate these laboratories. The development of a safe surrogate would speed research and reduce risk to researchers. Two highly conserved Ebola gene segments—from the glycoprotein and nucleoprotein genes—were designed with modifications preventing expression while maintaining sequence integrity, spliced into high copy number plasmids, cloned into E.coli, and tested for stability, safety, and potential research applications. The surrogates were stable over 2-3 months, had a negligible mutation rate (<0.165% over the experiment), and were detectable in human blood down to 5.8E3-1.17E4 surrogates/mL. These protocols could be used to safely simulate other pathogens and promote infectious disease treatment and detection research.
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Date Issued
2018
PURL
http://purl.flvc.org/fau/fd/FA00013015
Subject Headings
Ebolavirus, Infectious disease research, Ebola virus disease, Synthetic biology
Format
Document (PDF)