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Title: THP-1 Monocyte Differentiation and Activation.
Creator: Blaise, Danice, Hartmann, James X.
Abstract/Description: FAU's Office of Undergraduate Research and Inquiry hosts an annual symposium where students engaged in undergraduate research may present their findings either through a poster presentation or an oral presentation.
Date Issued: 2011
PURL: http://purl.flvc.org/fau/fd/FA00005429
Format: Document (PDF)

Title: Are filtration rates for the rough tunicate Styela plicata independent of weight or size?.
Creator: Draughon, Lisa D., Scarpa, John, Hartmann, James X.
Date Issued: 2010
PURL: http://purl.flvc.org/FCLA/DT/3352943
Subject Headings: Filtration, Tunicata, Styela, Bioremediation, Estuaries, Microalgae, Bacteria
Format: Document (PDF)

Title: Potential estuarine water quality improvement via marine invertebrate bioremediation.
Creator: Draughon, Lisa D., Scarpa, John, Keating, Patricia, Hartmann, James X.
Date Issued: 2008
PURL: http://purl.flvc.org/fau/fd/FA00007399
Subject Headings: Estuaries, Water quality, Marine invertebrates, Bioremediation
Format: Document (PDF)

Title: Analysis of gene expression profiles from normal and chronic lymphocytic leukemia white blood cells.
Creator: Chapp, Robert James., Florida Atlantic University, Hartmann, James X.
Abstract/Description: This project used a bioinformatics approach to identify the genetic differential expression of chronic lymphocytic leukemia (CLL) white blood cells as compared to normal white blood cells. Several public access databases and data mining tools were used to collect these data. The data collected was validated by independent bioinformatics databases and the methodology was supported by previously published "gene chip" differential expression data. This research identifies a pattern of...
Show moreThis project used a bioinformatics approach to identify the genetic differential expression of chronic lymphocytic leukemia (CLL) white blood cells as compared to normal white blood cells. Several public access databases and data mining tools were used to collect these data. The data collected was validated by independent bioinformatics databases and the methodology was supported by previously published "gene chip" differential expression data. This research identifies a pattern of differential gene expression specific to CLL white blood cells that can be used for the early diagnosis of CLL. The study also identifies the probable genetic origin for the low expression of tyrosine kinase and IgM immunoglobulin observed in CLL B-cells. Also presented are genes associated with chromosomal regions previously reported as deleted in a high percentage of CLL cases. These data can be used in further research and for the treatment of CLL.
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Date Issued: 2003
PURL: http://purl.flvc.org/fcla/dt/13024
Subject Headings: Lymphocytic leukemia, Gene expression, Bioinformatics, Leucocytes
Format: Document (PDF)

Title: The establishment and study of two elasmobranch cell lines.
Creator: Poyer, James Christopher., Florida Atlantic University, Hartmann, James X.
Abstract/Description: The present study describes the first cell lines produced from members of class Chondrichthyes. Explants of brain tissue from Carcharhinus falciformis (silky shark) and Ginglymostoma cirratum (nurse shark) were incubated in a mammalian medium modified with the addition of urea, trimethylamine N-oxide, NaCl, and bovine serum. Primary monolayers were passaged with 0.025% trypsin in a modified saline solution. Silky shark cells grew optimally at 29C. The population doubling time for C....
Show moreThe present study describes the first cell lines produced from members of class Chondrichthyes. Explants of brain tissue from Carcharhinus falciformis (silky shark) and Ginglymostoma cirratum (nurse shark) were incubated in a mammalian medium modified with the addition of urea, trimethylamine N-oxide, NaCl, and bovine serum. Primary monolayers were passaged with 0.025% trypsin in a modified saline solution. Silky shark cells grew optimally at 29C. The population doubling time for C. falciformis cells at passage 29 was 67 hours. For G. cirratum cells at passage 6 the population doubling was 84 hours. Silky shark cells grew over a broad range of osmolalities from 315 mOsm to a 1664 mOsm with optimal growth at 650 mOsm. A medium containing 10% dimethylsulfoxide allowed for cryopreservation with greater than 65% viability upon recovery. Current theories of elasmobranch osmoregulation are discussed in light of experimental data collected from studies conducted on the silky shark cell line.
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Date Issued: 1992
PURL: http://purl.flvc.org/fcla/dt/14795
Subject Headings: Sharks, Cell culture, Chondrichthyes
Format: Document (PDF)

Title: Enhanced CpG Activated Macrophage Killing of 3-Bromopyruvate Pre-treated 4T1 Breast Cancer Cells.
Creator: Rumicha, Dawit, Liddle, Genevieve M., Hartmann, James X., Office of Undergraduate Research and Inquiry
Abstract/Description: A common feature of breast cancer cells is the evasion of singular treatments by using the Warburg Effect, a process of metabolic ATP production through rapid anaerobic glycolysis. Cancer research has transitioned to an investigation of combination therapies to combat cancer. In our study, we seek to metabolically inhibit cancer cells before application of immunogenic killing. The Warburg Effect was targeted with 3-Bromopyruvate (3-BP), which blocks Glyceraldehyde 3-Phosphate Dehydrogenase ...
Show moreA common feature of breast cancer cells is the evasion of singular treatments by using the Warburg Effect, a process of metabolic ATP production through rapid anaerobic glycolysis. Cancer research has transitioned to an investigation of combination therapies to combat cancer. In our study, we seek to metabolically inhibit cancer cells before application of immunogenic killing. The Warburg Effect was targeted with 3-Bromopyruvate (3-BP), which blocks Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) production. Treatment with 3-BP yielded up to 86.5% cancer cell death. Glycolytic inhibition renders cancer cells metabolically stressed, which may enable an effective immune response. Our hypothesis was that CpG activated macrophage will possess tumoricidal potential to target metabolically stressed cancer cells. Macrophages and CpG cultivation alone yielded a significant immune response. We sought to find a synergistic effect of 3-BP induced killing susceptibility with CpG activated macrophages may lead to an effective method of combination therapy.
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Date Issued: 2017
PURL: http://purl.flvc.org/fau/fd/FA00005634
Subject Headings: College students --Research --United States.
Format: Document (PDF)

Title: Channel catfish virus disease vaccine and method of preparation thereof and method of immunization therewith.
Creator: Hartmann, James X., Noga, Edward J., Florida Atlantic University
Date Issued: 1980-08
PURL: http://purl.flvc.org/fcla/dt/15860
Format: Document (PDF)

Title: Colloidal gold labeling of monoclonal antibodies for billfish species identification.
Creator: Duan, Fei., Florida Atlantic University, Hartmann, James X.
Abstract/Description: This study established a lateral flow competitive immunoassay with a colloidal gold-monoclonal antibody probe for the qualitative detection of sailfish serum albumin. The test involves mixing a tissue homogenate with a colloidal gold-monoclonal antibody suspension and applying the mixture to a strip of plastic-backed nitrocellulose membrane. The presence of albumin in a target sample competed with adsorbed antigen and prevented the appearance of a pink color on the nitrocellulose membrane. A...
Show moreThis study established a lateral flow competitive immunoassay with a colloidal gold-monoclonal antibody probe for the qualitative detection of sailfish serum albumin. The test involves mixing a tissue homogenate with a colloidal gold-monoclonal antibody suspension and applying the mixture to a strip of plastic-backed nitrocellulose membrane. The presence of albumin in a target sample competed with adsorbed antigen and prevented the appearance of a pink color on the nitrocellulose membrane. A non-target sample yielded a pink color when gold-labeled monoclonal antibodies bound to sailfish albumin previously absorbed to the nitrocellulose. Three gold particle sizes for antibody conjugation were evaluated and of these, 41 nm was optimal. The optimal pH for conjugation of anti-sailfish antibody to colloidal gold was 7.0. The assay requires only five minutes to perform and utilizes two solutions.
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Date Issued: 2000
PURL: http://purl.flvc.org/fcla/dt/15789
Subject Headings: Billfishes, Colloidal gold, Immunogold labeling
Format: Document (PDF)

Title: Characterization and distribution of parvalbumin from selected fish species.
Creator: Ross, Cliff Ian., Florida Atlantic University, Hartmann, James X., Mari, Frank
Abstract/Description: Parvalbumins are acidic calcium-binding proteins found in large quantities in the white muscle fibers of cold-blooded vertebrates. Fish display two to seven parvalbumin isotypes that are species specific and thus constitute a valuable tool in the study of phylogenetic relationships, and as a specific biomarker for fish identification. Parvalbumins were isolated from a selected sample of marine teleosts and elasmobranchs. Purified isotypes were characterized via HPLC (gel filtration, and ion...
Show moreParvalbumins are acidic calcium-binding proteins found in large quantities in the white muscle fibers of cold-blooded vertebrates. Fish display two to seven parvalbumin isotypes that are species specific and thus constitute a valuable tool in the study of phylogenetic relationships, and as a specific biomarker for fish identification. Parvalbumins were isolated from a selected sample of marine teleosts and elasmobranchs. Purified isotypes were characterized via HPLC (gel filtration, and ion exchange), and electrophoresis (IEF, and SDS-PAGE). An indirect immunoassay was developed for the parvalbumin isotypes using monoclonal antibodies directed against the highly conserved calcium-binding site. Parvalbumins from selected fish species are compared and contrasted by standard biochemical and immunological methods.
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Date Issued: 1998
PURL: http://purl.flvc.org/fcla/dt/15536
Subject Headings: Fishes, Calcium-binding proteins, Globular proteins
Format: Document (PDF)

Title: Detection and purification of lupus B cells.
Creator: Saccocio, Seth., Florida Atlantic University, Hartmann, James X.
Abstract/Description: This study developed a method for positive selection of anti-single stranded (ss)DNA B cells from peripheral blood mononuclear cells (PBMCs) using a twenty nucleotide oligomer of deoxythymidine (oligo(dT) 20mer), coupled to microscopie magnetic beads. Oligo(dT) 20mer specificity for plasma anti-ssDNA antibody (Ab) was established through indirect enzyme linked immunosorbent assay (ELISA) and flow cytometry. A novel method for semi-quantitative detection of ssDNA specific, B cells from PBMC...
Show moreThis study developed a method for positive selection of anti-single stranded (ss)DNA B cells from peripheral blood mononuclear cells (PBMCs) using a twenty nucleotide oligomer of deoxythymidine (oligo(dT) 20mer), coupled to microscopie magnetic beads. Oligo(dT) 20mer specificity for plasma anti-ssDNA antibody (Ab) was established through indirect enzyme linked immunosorbent assay (ELISA) and flow cytometry. A novel method for semi-quantitative detection of ssDNA specific, B cells from PBMC purified peripheral blood that utilized flow cytometric analysis of oligo(dT) 20mer Texas Red labeled cells was developed. Qualitative purification of ssDNA specific, B cells using oligo(dT) coupled magnetic beads was determined through light microscopy and flow cytometric analysis of positive sclected cell populations. Cross-reaetivity of oligo(dT) 20mer with receptors distinct from membrane Ab, resulted in the use of oligo(dC) 20mer as a useful blocking agent. Results show anti-ssDNA Ab titer does not correlate with numbers of peripheral blood ssDNA specific, B cells.
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Date Issued: 2003
PURL: http://purl.flvc.org/fcla/dt/13086
Subject Headings: Systemic lupus erythematosus, B cells, DNA antibodies
Format: Document (PDF)

Title: EFFECTIVENESS OF ANTI-HERPETIC COMPOUNDS ON PACHECO'S DISEASE VIRUS OF PSITTACINES.
Creator: AVERY, PATRICIA OTTENS., Florida Atlantic University, Hartmann, James X.
Abstract/Description: Three antiviral compounds, 5-iodo-2' -deoxyuridine (IUDR), 9-(2-hydroxyethoxymethyl )guanine (acyclovir) and methylglyoxal-bis-(guanylhydrazone) MGBG were tested for their effectiveness against Pacheco's disease virus (PDV). IUDR caused a fifty percent reduction in plaque formation (PR50) at 0 .8 μM in chicken embryo fibroblasts while acyclovir showed a PR50 of 6.6 μM. Plaque inhibition assays showed a significant decrease in plaque size (1.25 mm to 0.45 mm) at 10 μM acyclovir and (1.3 mm to...
Show moreThree antiviral compounds, 5-iodo-2' -deoxyuridine (IUDR), 9-(2-hydroxyethoxymethyl )guanine (acyclovir) and methylglyoxal-bis-(guanylhydrazone) MGBG were tested for their effectiveness against Pacheco's disease virus (PDV). IUDR caused a fifty percent reduction in plaque formation (PR50) at 0 .8 μM in chicken embryo fibroblasts while acyclovir showed a PR50 of 6.6 μM. Plaque inhibition assays showed a significant decrease in plaque size (1.25 mm to 0.45 mm) at 10 μM acyclovir and (1.3 mm to 0.5 mm) at both 1 μM and 10 μM of IUDR. MGBG did not reduce virus replication vitro, and an apparent reduction of virus growth in vivo was probably due to toxicity. However, in vitro toxicity studies did not show any significant reduction in cell growth with any of the compounds tested.
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Date Issued: 1983
PURL: http://purl.flvc.org/fcla/dt/14145
Subject Headings: Herpesviruses, Birds--Diseases, Psittacosis
Format: Document (PDF)

Title: Cytokinetic effects of platinum (II) polyamines on three different cell lines using flow cytometry.
Creator: Fitch, Claire E., Florida Atlantic University, Hartmann, James X., Louda, Deborah W.
Abstract/Description: The association between cytotoxicity and cell cycle perturbation caused by cisplatin and two platinum (II) polyamines, containing a methotrexate or a 2-chloro-p-phenylenediamine component, was investigated in human ovarian carcinoma cells (CAOV3), murine Balb 3T3 fibroblasts and Chinese ovarian hamster cells by flow cytometric DNA/protein, DNA/RNA and DNA degradation assays. Continuous in vitro drug exposure caused a time- and dose-dependent suppression of growth. Flow cytometric analysis...
Show moreThe association between cytotoxicity and cell cycle perturbation caused by cisplatin and two platinum (II) polyamines, containing a methotrexate or a 2-chloro-p-phenylenediamine component, was investigated in human ovarian carcinoma cells (CAOV3), murine Balb 3T3 fibroblasts and Chinese ovarian hamster cells by flow cytometric DNA/protein, DNA/RNA and DNA degradation assays. Continuous in vitro drug exposure caused a time- and dose-dependent suppression of growth. Flow cytometric analysis revealed a multiplicity of cytokinetic perturbations, the most important of which was Go/G1 depletion and S phase arrest. The latter correlated with inhibition of DNA synthesis and subsequent inhibition of RNA and protein synthesis. Prolonged S phase arrest concomitant with DNA degradation, followed by RNA and protein degradation, resulted in cell death via apoptosis. The platinum polymers appear to be effective in vitro chemotherapeutic agents. The study of cytokinetic perturbations is useful in determining the relative potency of antitumor drugs and in monitoring drug effectiveness during treatment.
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Date Issued: 1999
PURL: http://purl.flvc.org/fcla/dt/15626
Subject Headings: Cisplatin, Cell lines, Flow cytometry, Polymers
Format: Document (PDF)

Title: Monoclonal antibodies and blue marlin (Makaira nigricans) identification.
Creator: Paniker, Lakshmi., Florida Atlantic University, Hartmann, James X.
Abstract/Description: Spleenocytes from Balb/c mouse immunized with purified blue marlin albumin were fused with X 63 myeloma cells in a polyethylene glycol aided fusion. The resulting fusomas were screened for anti-blue marlin activity and a potential blue marlin specific hybridoma clone designated 605D was isolated and expanded in cell culture. While testing the reactivity of monoclonal antibody secreted by clone 605D against pooled serum samples of blue marlin, white marlin and sailfish in indirect ELISA, 605D...
Show moreSpleenocytes from Balb/c mouse immunized with purified blue marlin albumin were fused with X 63 myeloma cells in a polyethylene glycol aided fusion. The resulting fusomas were screened for anti-blue marlin activity and a potential blue marlin specific hybridoma clone designated 605D was isolated and expanded in cell culture. While testing the reactivity of monoclonal antibody secreted by clone 605D against pooled serum samples of blue marlin, white marlin and sailfish in indirect ELISA, 605D could differentiate blue marlin from white marlin and sailfish clearly. The crossreactivity to white marlin was minimised by diluting sera samples and antibody solutions. However, when individual billfish serum samples and heart tissue homogenates were tested 605D could not differentiate between the two marlin species, though crossreactivity to SF was minimal.
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Date Issued: 1996
PURL: http://purl.flvc.org/fcla/dt/15347
Subject Headings: Blue marlin--Identification, Fishes--Immunology, Immunology--Technique
Format: Document (PDF)

Title: Evaluation of monoclonal antibody technology for the species-specific identification of Centropomus undecimalis.
Creator: Potter, Christopher Samuel., Florida Atlantic University, Hartmann, James X.
Abstract/Description: A fusion was conducted using spleen cells from a hyperimmunized Balb/C mouse and a murine myeloma cell line, in an attempt to create monoclonal antibodies specific to parvalbumin from Centropomus undecimalis. The fusion protocol utilized a 24 well culture plate protocol. Initial results indicated the successful generation of antibody producing hybridomas specific to the desired parvalbumin; however, attempts to isolate and clone these cells were unsuccessful. The study results clearly...
Show moreA fusion was conducted using spleen cells from a hyperimmunized Balb/C mouse and a murine myeloma cell line, in an attempt to create monoclonal antibodies specific to parvalbumin from Centropomus undecimalis. The fusion protocol utilized a 24 well culture plate protocol. Initial results indicated the successful generation of antibody producing hybridomas specific to the desired parvalbumin; however, attempts to isolate and clone these cells were unsuccessful. The study results clearly demonstrate some potential drawbacks to using the 24-well fusion protocol versus a 96-well protocol. The 24-well protocol seems to favor rapidly growing non-secreting cells due to the lower dilutions involved. This study was successful in isolating murine polyclonal antiserum reactive in isolating murine polyclonal antiserum reactive with piscine parvalbumin. Further, initial cultures of hybridomas were able to distinguish C. undecimalis from several other species of fishes thus confirming the suitability of parvalbumin as a biomaker protein.
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Date Issued: 1998
PURL: http://purl.flvc.org/fcla/dt/15552
Subject Headings: Monoclonal antibodies, Snook--Identification
Format: Document (PDF)

Title: Evaluation of the specificity and sensitivity of monoclonal antibodies produced against sailfish albumin.
Creator: Rossi, Edmund Anthony., Florida Atlantic University, Hartmann, James X.
Abstract/Description: Monoclonal antibodies (MAbs) were produced against sailfish (Istiophorus platypterus) albumin and investigated for their potential use in species identification. Balb/c mice were immunized with albumin purified from sailfish serum. Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) for reactivity with the purified albumins of sailfish, blue marlin (Makaira nigricans) and white marlin (Tetrapturus albidus). MAbs from 18 different hybridomas exhibited activity against...
Show moreMonoclonal antibodies (MAbs) were produced against sailfish (Istiophorus platypterus) albumin and investigated for their potential use in species identification. Balb/c mice were immunized with albumin purified from sailfish serum. Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) for reactivity with the purified albumins of sailfish, blue marlin (Makaira nigricans) and white marlin (Tetrapturus albidus). MAbs from 18 different hybridomas exhibited activity against sailfish albumin. Sixteen MAbs showed cross-reactivity with the marlin species. Two MAbs displayed distinct specificity for sailfish albumin. One of these MAbs (M2D1) was conjugated to horseradish peroxidase to construct an ELISA for the identification of sailfish from serum. The assay correctly identified 18 sailfish from 36 billfish samples. The MAb-peroxidase conjugate was highly specific toward sailfish with no detectable reaction against the heterologous species.
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Date Issued: 1992
PURL: http://purl.flvc.org/fcla/dt/14801
Subject Headings: Sailfish, Monoclonal antibodies
Format: Document (PDF)

Title: Immunoaffinity purification of albumin from fish serum.
Creator: Apter, Christine V., Florida Atlantic University, Hartmann, James X.
Abstract/Description: This study describes an affinity method which purifies albumin from fish serum. Hybridoma clones producing antibodies specific to fish albumin have been screened and propagated and their antibodies coupled to a cross-linked agarose gel matrix which has been activated with N-hydroxysuccinimide. The resulting gel with its ligand attached was then used to purify albumin from fish serum. The procedure was optimized for coupling, sample application and elution. The coupling optimization using...
Show moreThis study describes an affinity method which purifies albumin from fish serum. Hybridoma clones producing antibodies specific to fish albumin have been screened and propagated and their antibodies coupled to a cross-linked agarose gel matrix which has been activated with N-hydroxysuccinimide. The resulting gel with its ligand attached was then used to purify albumin from fish serum. The procedure was optimized for coupling, sample application and elution. The coupling optimization using varying pH values resulted in over 90% coupling efficiencies. The sample application optimization showed yields of 2 mg of protein per ml of serum at 1:5 dilution of sample. Elution conditions were evaluated using varying pH and chaotropic agent concentrations. The optimal elution conditions (pH 4.0) resulted in a yield of 0.2 mg/ml of gel with several impurities. Elution at pH 3.0 resulted in a very pure product with low yield (.02 mg/ml of gel) and a loss of activity.
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Date Issued: 1998
PURL: http://purl.flvc.org/fcla/dt/15568
Subject Headings: Serum albumin--Purification, Immune serums
Format: Document (PDF)

Title: Rapid, immunologic identification of istiophorid fishes from minute tissue samples.
Creator: Shepard, Scot Robert., Florida Atlantic University, Hartmann, James X.
Abstract/Description: A monoclonal antibody (MAb) produced against sailfish (Istiophorus albicans) serum albumin (SFA)/(Rossi et al. 1992) was used in conjunction with rabbit polyclonal antibodies (PAbs) to formulate a sandwich-style enzyme immunoassay (sEIA). The sEIA specifically identifies sailfish tissues utilizing samples such as heart, kidney, white muscle, red muscle, and blood serum. The assay is sensitive to 20ng of SFA. Of the tissues tested, kidney yields the strongest signal. The entire assay is...
Show moreA monoclonal antibody (MAb) produced against sailfish (Istiophorus albicans) serum albumin (SFA)/(Rossi et al. 1992) was used in conjunction with rabbit polyclonal antibodies (PAbs) to formulate a sandwich-style enzyme immunoassay (sEIA). The sEIA specifically identifies sailfish tissues utilizing samples such as heart, kidney, white muscle, red muscle, and blood serum. The assay is sensitive to 20ng of SFA. Of the tissues tested, kidney yields the strongest signal. The entire assay is carried out in a 3cc syringe and, excluding sample preparation, can be executed in 30 minutes. No specialized equipment or training is required. MAbs were also produced against serum albumin purified from white marlin (Tetrapturus albidus) and Atlantic blue marlin (Makaira nigricans). These Mabs were examined for their utility in identification assays for these species.
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Date Issued: 1994
PURL: http://purl.flvc.org/fcla/dt/15052
Subject Headings: Monoclonal antibodies, Sailfish, Billfishes--Identification, Billfishes
Format: Document (PDF)

Title: Variability in the venom of Conus regius.
Creator: Uribe-Benninghoff, Alejandro., Florida Atlantic University, Hartmann, James X., Mari, Frank
Abstract/Description: The venom of two different geographical populations of Conus regius has been isolated and characterized. Comparisons between the chromatographic profiles of the venom of these two populations exhibited similarities and differences among the venom's constituents. MALDI-TOF and PCR analysis techniques ratified the differences present in the venom of both populations. It is postulated that these differences could reflect the rapid adaptive nature of cone snails in an actual stage of speciation....
Show moreThe venom of two different geographical populations of Conus regius has been isolated and characterized. Comparisons between the chromatographic profiles of the venom of these two populations exhibited similarities and differences among the venom's constituents. MALDI-TOF and PCR analysis techniques ratified the differences present in the venom of both populations. It is postulated that these differences could reflect the rapid adaptive nature of cone snails in an actual stage of speciation. Molecular weights of the venom's constituents were compared with those of patented conopeptides in the Swiss Protein Database. Results of this comparison indicated that a number of the peptides isolated for both of the populations of C. regius had the same molecular weight as other patented conopeptides. In combination with the PCR analysis of these conopeptides, it has been proposed that some of the venom constituents of the venom of C. regius could have pharmacological applications for vertebrate systems.
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Date Issued: 1999
PURL: http://purl.flvc.org/fcla/dt/15746
Subject Headings: Conus--Venom
Format: Document (PDF)

Title: Comparative study of shark (Carcharinus falciformis) and mammalian (Mesocricetus auratus) cells exposed to chemical carcinogens.
Creator: Ritchey, Donna Mae., Florida Atlantic University, Hartmann, James X.
Abstract/Description: It is not known by what mechanism members of the subclass Elasmobranchii derive their natural resistance to neoplasia. To determine if this resistance is at the cellular level, cultures of shark (Carcharinus falciformis) cells were exposed to varying concentrations of the chemical carcinogens methanesulfonic acid ethyl ester (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Treated cells were assayed for cytotoxicity, formation of transformed foci and growth in agarose. It was found that...
Show moreIt is not known by what mechanism members of the subclass Elasmobranchii derive their natural resistance to neoplasia. To determine if this resistance is at the cellular level, cultures of shark (Carcharinus falciformis) cells were exposed to varying concentrations of the chemical carcinogens methanesulfonic acid ethyl ester (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Treated cells were assayed for cytotoxicity, formation of transformed foci and growth in agarose. It was found that a greater chemical concentration was necessary to reach LC50 in shark cells than in mammalian cells (BHK21 Cl13). Shark cell cultures developed no transformed foci over a range of chemical concentrations of EMS whereas foci formed at all concentrations in BHK control cultures exposed to EMS. Treated shark cells did not develop transformed colonies in agarose, but the mammalian cells readily formed colonies. These results suggest that the natural resistance of elasmobranchs to neoplasia could be partially, if not entirely, regulated at the cellular level.
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Date Issued: 1991
PURL: http://purl.flvc.org/fcla/dt/14708
Subject Headings: Carcharhinus, Carcinogens, Golden hamster
Format: Document (PDF)

Title: A study of conjugated monoclonal antibodies in an immunoassay for fish species identification.
Creator: Yu, Wenjie, Florida Atlantic University, Hartmann, James X.
Abstract/Description: Atlantic billfishes (family Istiophoridae) are overexploited and often illegally harvested. To address both of these problems, a rapid means of identifying billfish carcasses is needed. This thesis describes a simple and rapid Nalge Nunc-Immuno(TM) Stick-based direct sandwich assay for sailfish identification that can be performed in the field. A species-specific anti-sailfish monoclonal antibody, covalently bound to the Nalge Nunc-Immuno(TM) Stick's polystyrene surface, was used to capture a...
Show moreAtlantic billfishes (family Istiophoridae) are overexploited and often illegally harvested. To address both of these problems, a rapid means of identifying billfish carcasses is needed. This thesis describes a simple and rapid Nalge Nunc-Immuno(TM) Stick-based direct sandwich assay for sailfish identification that can be performed in the field. A species-specific anti-sailfish monoclonal antibody, covalently bound to the Nalge Nunc-Immuno(TM) Stick's polystyrene surface, was used to capture a biomarker molecule---serum albumin---from sailfish tissue samples. This antibody-antigen interaction was visualized by utilizing peroxidase-conjugated anti-billfish monoclonal antibodies or polyclonal antibodies together with a precipitating substrate. This technique successfully differentiated between sailfish and other fishes within 15 minutes, with a high degree of sensitivity and accuracy. This assay has many potential applications for species identification.
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Date Issued: 2000
PURL: http://purl.flvc.org/fcla/dt/15794
Subject Headings: Monoclonal antibodies, Fishes--Identification, Fishes--Immunology
Format: Document (PDF)

FAU Theses and Dissertations (30)	+ -
HBOI Faculty Contributions (2)	+ -
FAU Undergraduate Student Research (2)	+ -
Florida Atlantic University Patents (1)	+ -

Monoclonal antibodies (4)	+ -
Calcitriol (3)	+ -
Billfishes (2)	+ -
Bioremediation (2)	+ -
Breast--Cancer--Treatment. (2)	+ -

Cancer--Molecular aspects. (2)	+ -
DNA antibodies (2)	+ -
Dienogest (2)	+ -
Endometriosis (2)	+ -
Estuaries (2)	+ -
Fishes--Immunology (2)	+ -
Sailfish (2)	+ -
Systemic lupus erythematosus (2)	+ -
Systemic lupus erythematosus--Molecular aspects (2)	+ -
Algal blooms -- Florida -- Indian River Lagoon (1)	+ -
Anti-DNA antibodies (1)	+ -
Antibodies, Catalytic (1)	+ -
Antibodies--isolation & purification (1)	+ -
Apoptosis. (1)	+ -
Autoantibodies--Analysis (1)	+ -
Autoimmune diseases--Research (1)	+ -
B cells (1)	+ -
Bacteria (1)	+ -
Bacterial genetics (1)	+ -
Billfishes--Identification (1)	+ -
Bioinformatics (1)	+ -
Biological interfaces. (1)	+ -
Biological response modifiers. (1)	+ -
Birds--Diseases (1)	+ -
Blue marlin--Identification (1)	+ -

Electronic Thesis or Dissertation (30)	+ -
article (2)	+ -
poster (2)	+ -
patent (1)	+ -

Hartmann, James X. (35)	+ -
Florida Atlantic University (31)	+ -
Charles E. Schmidt College of Science (12)	+ -
Department of Biological Sciences (12)	+ -
Mari, Frank (3)	+ -

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