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- Title
- 2014-2015 Program Review Biological Sciences.
- Creator
- Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for Charles E. Schmidt College of Science, Florida Atlantic University.
- Date Issued
- 2014-2015
- PURL
- http://purl.flvc.org/fau/fd/FA00007700
- Format
- Document (PDF)
- Title
- 2013-2014 Program Review Biological Sciences.
- Creator
- Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for Charles E. Schmidt College of Science, Florida Atlantic University.
- Date Issued
- 2013-2014
- PURL
- http://purl.flvc.org/fau/fd/FA00007693
- Format
- Document (PDF)
- Title
- 2012-2013 Program Review Biological Sciences.
- Creator
- Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for Charles E. Schmidt College of Science, Florida Atlantic University.
- Date Issued
- 2012-2013
- PURL
- http://purl.flvc.org/fau/fd/FA00007686
- Format
- Document (PDF)
- Title
- 2009-2010 Program Review Biological Sciences.
- Creator
- Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for Charles E. Schmidt College of Science, Florida Atlantic University.
- Date Issued
- 2009-2010
- PURL
- http://purl.flvc.org/fau/fd/FA00007672
- Format
- Document (PDF)
- Title
- 2010-2011 Program Review Biological Sciences.
- Creator
- Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for Charles E. Schmidt College of Science, Florida Atlantic University.
- Date Issued
- 2010-2011
- PURL
- http://purl.flvc.org/fau/fd/FA00007679
- Format
- Document (PDF)
- Title
- 2015-2016 Program Review Biological Sciences.
- Creator
- Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for Charles E. Schmidt College of Science, Florida Atlantic University.
- Date Issued
- 2015-2016
- PURL
- http://purl.flvc.org/fau/fd/FA00007707
- Format
- Document (PDF)
- Title
- 2016-2017 Program Review Biological Sciences.
- Creator
- Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for Charles E. Schmidt College of Science, Florida Atlantic University.
- Date Issued
- 2016-2017
- PURL
- http://purl.flvc.org/fau/fd/FA00007714
- Format
- Document (PDF)
- Title
- Anticarcinogenic effects of genistein and anthocyanin extract in MCF-7 human breast cancer cells.
- Creator
- Stinson, Corine M., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
This study investigated potential apoptotic and anti-proliferative effects of the phytochemicals, genistein and anthocyanin extract, as single and combined treatments in MCF-7 human breast cancer cells. Cells were exposed to single and combined treatments with the phytochemiclas for 48 and 72 hours. Cell viability was assessed using the MTT bioassay. Apoptosis induction was assessed using acridine orange ethidium bromide and rhodamine 123 ethidium bromide fluorescence assays. Both singe and...
Show moreThis study investigated potential apoptotic and anti-proliferative effects of the phytochemicals, genistein and anthocyanin extract, as single and combined treatments in MCF-7 human breast cancer cells. Cells were exposed to single and combined treatments with the phytochemiclas for 48 and 72 hours. Cell viability was assessed using the MTT bioassay. Apoptosis induction was assessed using acridine orange ethidium bromide and rhodamine 123 ethidium bromide fluorescence assays. Both singe and combination treatments induced dose- and time-dependent apoptotic cell death in MCF-7 cells. The percentage of apoptosis was higher in combination treatments than single treatments with either phytochemical, although the difference was not statistically significant. The combination of genistein and anthocyanin extract peaked in efficacy at 48 hours of treatment, to exhibit significantly greater (P<. O5) dose- and time-dependent cell cytotoxicity than single treatments. This study reveals potential chemopreventive implications for the complementary effects of genistein and anthocyanin extract.
Show less - Date Issued
- 2011
- PURL
- http://purl.flvc.org/FAU/3320108
- Subject Headings
- Phytochemicals, Therapeutic use, Phytoestrogens, Physiological effect, Breast, Cancer, Risk factors, Breast, Cancer, Treatment, Probiotics, Cancer, Chemoprevention, Antioxidants, Therapeutic use
- Format
- Document (PDF)
- Title
- Anatomy and physiology of the elasmobranch olfactory system.
- Creator
- Meredith, Tricia L., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
The olfactory system is the most highly developed system for molecular sensing in vertebrates. Despite their reputation for being particularly olfactory driven, little is known about how this sense functions in elasmobranch fishes. The goal of this dissertation was to examine the morphology and physiology of elasmobranchs to compare their olfactory system with teleost fishes and more derived vertebrates. To test the hypotheses that elasmobranchs possess greater olfactory sensitivities than...
Show moreThe olfactory system is the most highly developed system for molecular sensing in vertebrates. Despite their reputation for being particularly olfactory driven, little is known about how this sense functions in elasmobranch fishes. The goal of this dissertation was to examine the morphology and physiology of elasmobranchs to compare their olfactory system with teleost fishes and more derived vertebrates. To test the hypotheses that elasmobranchs possess greater olfactory sensitivities than teleosts and that lamellar surface area is correlated to sensitivity, I compared the surface area of the olfactory lamellae and the olfactory sensitivities of five phylogenetically diverse elasmobranch species. The olfactory thresholds reported here (10-9 to 10-6 M) were comparable to those previously reported for teleosts and did not correlate with lamellar surface area. Since aquatic species are subject to similar environmental amino acid levels, they appear to have converged upon similar amino acid sensitivities. To test the hypothesis that elasmobranchs are able to detect bile salt odorants despite lacking ciliated olfactory receptor neurons (ORNs), the type of ORN that mediates bile salt detection in the teleosts, I quantified the olfactory specificity and sensitivity of two elasmobranch species to four, teleost-produced C24 bile salts. Both species responded to all four bile salts, but demonstrated smaller relative responses and less sensitivity compared to teleosts and agnathans. This may indicate that elasmobranchs don't rely on bile salts to detect teleost prey. Also, the olfactory system of elasmobranchs contains molecular olfactory receptors for bile salts independent of those that detect amino acids, similar to teleosts., In some elasmobranch species, each olfactory bulb (OB) is physically partitioned into two hemi-bulbs; however, the functional significance of this morphology is not fully understood. The organization of the OBs in three species with varying OB morphologies was examined to test the hypothesis that the elasmobranch OB is somatotopically arranged. Glomeruli in the OB received projections from ORNs in 3-4 olfactory lamellae situated immediately anterior. These results indicate a somatotopic arrangement of the elasmobranch OB, which may be unique among vertebrate olfactory systems and potentially led to the hemi-OB morphology.
Show less - Date Issued
- 2011
- PURL
- http://purl.flvc.org/FAU/3333058
- Subject Headings
- Condrichthyes, Sense organs, Condrichthyes, Physiology, Sharks, Physiology, Adaptation (Biology), Aquatic animals, Physiology
- Format
- Document (PDF)
- Title
- Biomonitoring of hypoxia and sulfide stress in three sub-tropical seagrasses.
- Creator
- Irwin, Connor., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Hypoxia and sulfide exposure, increased using glucose, are considered major environmental stressors in seagrass communities. Quantum efficiency, total soluble protein and catalase activity were quantified to evaluate the applicability of each of these bioindicators to detect environmental stress in three tropical seagrass species, Thalassia testudinum (Banks ex Kèoenig), Halodule wrightii (Ascherson) and Syringodium filiforme (Kuetz). Hypoxia + sulfide treatments significantly decreased the...
Show moreHypoxia and sulfide exposure, increased using glucose, are considered major environmental stressors in seagrass communities. Quantum efficiency, total soluble protein and catalase activity were quantified to evaluate the applicability of each of these bioindicators to detect environmental stress in three tropical seagrass species, Thalassia testudinum (Banks ex Kèoenig), Halodule wrightii (Ascherson) and Syringodium filiforme (Kuetz). Hypoxia + sulfide treatments significantly decreased the quantum efficiency of all three species, but showed no response in protein and catalase activity. Although no treatment effect was found, catalase activity was enhanced in T. testudinum leaves and H. wrightii roots relative to other tissues, while S. filiforme showed no location-specific catalase activity. These results indicate that quantum efficiency is a more sensitive indicator than protein and catalase activity to hypoxia and sulfide stress in seagrasses.
Show less - Date Issued
- 2010
- PURL
- http://purl.flvc.org/FAU/2976445
- Subject Headings
- Plant physiology, Environmental management, Seagrasses, Habitat, Environmental aspects, Sulfites, Physiological effect, Marine ecology
- Format
- Document (PDF)
- Title
- Characterization of the MHC II B of the bald eagle.
- Creator
- Smith, Andrew., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
The Major Histocompatibility Complex class II B (MHC II B) gene encodes a protein that is part of the adaptive immune system and critical for the non-self recognition ability of immune cells. This gene has been characterized in the Bald Eagle, ten unique alleles were found in two subpopulations at the geographic extremes of the range margins. Geographic genetic variation is suggested by the presence of population specific alleles. The results showed considerable divergence of groups of Bald...
Show moreThe Major Histocompatibility Complex class II B (MHC II B) gene encodes a protein that is part of the adaptive immune system and critical for the non-self recognition ability of immune cells. This gene has been characterized in the Bald Eagle, ten unique alleles were found in two subpopulations at the geographic extremes of the range margins. Geographic genetic variation is suggested by the presence of population specific alleles. The results showed considerable divergence of groups of Bald Eagle alleles when compared to alleles from other birds of prey. Particular codons within the exon II show signs of balancing selection driving the evolution of the MHC II B. Transcription data showed statistically significant differential expression of alleles. This can be interpreted as meaning a particular locus is being preferentially expressed in blood. The analysis of the polymorphism of this adaptive marker may aid managers of wildlife during this age of global climate change and the biodiversity crisis.
Show less - Date Issued
- 2010
- PURL
- http://purl.flvc.org/FAU/2979375
- Subject Headings
- Major histocompatibility complex, Cellular signal transduction, Immunogenetics, Genetic polymorphisms
- Format
- Document (PDF)
- Title
- Cleavage of brain glutamic acid decarboxylase 65 by calpain under pathological conditions.
- Creator
- Buddhala, Chandana, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Brain glutamic acid decarboxylase 65 (GAD65) catalyzes the rate-limiting step in the biosynthesis of the major inhibitory neurotransmitter-amino butyric acid (GABA) from the substrate L-glutamic acid. Severe lapse in GABA neurotransmission is one of the etiologies documented in the manifestation of certain neurodegenerative diseases such as epilepsy, Parkinson's disease, Huntington's disease etc. Because GAD65 synthesizes GABA, any modulation of GAD65, therefore, has direct implications on...
Show moreBrain glutamic acid decarboxylase 65 (GAD65) catalyzes the rate-limiting step in the biosynthesis of the major inhibitory neurotransmitter-amino butyric acid (GABA) from the substrate L-glutamic acid. Severe lapse in GABA neurotransmission is one of the etiologies documented in the manifestation of certain neurodegenerative diseases such as epilepsy, Parkinson's disease, Huntington's disease etc. Because GAD65 synthesizes GABA, any modulation of GAD65, therefore, has direct implications on the quanta of GABA released at the synapse. Hence, the major objective of this study was to focus on the regulation of GAD65, with special emphasis on investigating the proteolytic cleavage of fGAD65. Previously, we have shown in vitro that GAD65 was cleaved to form its truncated form (tGAD65), which was more active than the full length form (fGAD65). The enzyme responsible for cleavage was later identified as calpain. Calpain is known to cleave its substrates either under a transient physiologica l stimulus or upon a sustained pathological insult. However, the precise role of calpain cleavage of fGAD65 is poorly understood. In this study, we examined the cleavage of fGAD65 under a range of conditions encompassing both physiological and pathological aspects, including rats under ischemia/reperfusion insult, rat brain synaptosomes or primary neuronal cultures subjected to excitotoxic stimulation with KCl. It was observed that the formation of tGAD65 progressively increased with increasing stimulus concentration. More importantly, cleavage of synaptic vesicle (SV) - associated fGAD65 by calpain was demonstrated, and the resulting tGAD65 harboring the active site of the enzyme was detached from the SVs. Vesicular uptake of the newly synthesized GABA into the SVs was found to be reduced in calpain treated SVs. Furthermore, we also observed that the levels of tGAD65 in the focal cerebral ischemic rat brain tissue increased corresponding to the elevation of local glutamate indica, d by in vivo micro dialysis. Based on these observations, we conclude that calpain cleavage of fGAD65 occurs under pathological conditions.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3342053
- Subject Headings
- Glutamic acids, Antagonists, Proteolytic enzymes, Research, Cellular signal transduction, Calpain, Glutamic acid, Metabolism
- Format
- Document (PDF)
- Title
- Comprehensive study of the ZAD family of zinc finger transcription factors in Drosophila melanogaster.
- Creator
- Krystel, Joseph., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
The zinc finger associated domain (ZAD) family of transcription factors from Drosophila melanogaster is not well described in the literature, in part because it is very difficult to study by traditional mutagenesis screens. Bioinformatic studies indicate this is due to overlapping functions remaining after a recent evolutionary divergence. I set out to use in vitro-binding techniques to identify the characteristics of the ZAD family and test this theory. I have constructed glutathione S...
Show moreThe zinc finger associated domain (ZAD) family of transcription factors from Drosophila melanogaster is not well described in the literature, in part because it is very difficult to study by traditional mutagenesis screens. Bioinformatic studies indicate this is due to overlapping functions remaining after a recent evolutionary divergence. I set out to use in vitro-binding techniques to identify the characteristics of the ZAD family and test this theory. I have constructed glutathione S-transferase (GST)-ZAD domain chimeric proteins for use in pull down protein binding assays,and GST-Zinc finger (ZnF) array domain chimera for electrophoretic mobility shift assays (EMSA). Protein binding assays indicated two putative conserved interactors, similar to the analogous KRAB system in mammals. ... Competitive bindings were carried out to show a specificity of binding conferred by the identified conserved positions. While the consensus binding sites show relatively few similarities, the predicted target genes identified by the consensus binding sites show significant overlap. The nature of this overlap conforms to the known characteristics of the ZAD family but points to a more positive selection to maintain conservation of function.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3355627
- Subject Headings
- Cellular signal transduction, Drosophila melanogaster, Cytogenetics, Transcription factors, Zinc-finger proteins, Synthesis, Genetic transcription, Regulation, Gene expression
- Format
- Document (PDF)
- Title
- Consumptive effects of predatory fish reduce wetland crayfish (Procambarus spp.) recruitment and drive species turnover.
- Creator
- Kellogg, Christopher M., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Trade-offs in traits conferring success in permanent and ephemeral habitats are often at odds with few species being able to persist in both types of environments. I examined the effect of sunfish predators on two species of south Florida crayfish to establish the mechanism that limits one species, Procambarus alleni, to short-hydroperiod environments. The crayfish assemblage response to a gradient of sunfish predators and the effect of predation on P. fallax alone was examined. I also...
Show moreTrade-offs in traits conferring success in permanent and ephemeral habitats are often at odds with few species being able to persist in both types of environments. I examined the effect of sunfish predators on two species of south Florida crayfish to establish the mechanism that limits one species, Procambarus alleni, to short-hydroperiod environments. The crayfish assemblage response to a gradient of sunfish predators and the effect of predation on P. fallax alone was examined. I also examined the effects of sunfish on crayfish growth and quantified activity levels and risky behaviors of both crayfish species. P. alleni dominated at low sunfish densities but dominance shifted with increasing sunfish density. P. alleni was more active and likely to initiate risky behaviors, suggesting that sunfish predators remove the more active P. alleni, reducing their numbers disproportionally to those of P. fallax and allowing P. fallax to dominate crayfish assemblages in long-hydroperiod wetlands.
Show less - Date Issued
- 2011
- PURL
- http://purl.flvc.org/FAU/3171400
- Subject Headings
- Wetland ecology, Habitat selection, Marine ecosystem management, Predatory marine animals, Ecology
- Format
- Document (PDF)
- Title
- Dietary niche relationships of white ibis, tricolored heron and snowy egret nestlings in the northern Everglades.
- Creator
- Boyle, Robin A., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Food availability is the primary factor affecting the reproductive success in many species of birds. Diet composition can indicate diet quality, habitat use and niche requirements for breeding birds and may be variable across short and long-term time scales. Identifying primary prey types of nesting wading birds is important for the hydrologic restoration of wetlands. I collected nestling boluses during the 2008 and 2009 nesting seasons from three species of wading birds that nest in the...
Show moreFood availability is the primary factor affecting the reproductive success in many species of birds. Diet composition can indicate diet quality, habitat use and niche requirements for breeding birds and may be variable across short and long-term time scales. Identifying primary prey types of nesting wading birds is important for the hydrologic restoration of wetlands. I collected nestling boluses during the 2008 and 2009 nesting seasons from three species of wading birds that nest in the northern Everglades: White Ibis, Tricolored Herons and Snowy Egrets. White Ibis bolus composition was dominated by crayfish in both years, but exhibited some variation with landscape water depth in 2009; fish use was greatest when the wetland landscape was relatively dry. In contrast, the prey of Tricolored Herons and Snowy Egrets were primarily fish and their respective diets did not differ from one another in either fish species composition or size structure.
Show less - Date Issued
- 2010
- PURL
- http://purl.flvc.org/FAU/2953207
- Subject Headings
- Wetland ecology, Bird populations, Water birds
- Format
- Document (PDF)
- Title
- Elucidation of the features of the zinc finger associated domain (ZAD) family of transportation factors in Drosophila melanogaster.
- Creator
- Krystel, Joseph., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
The zinc finger associated domain (ZAD) containing family of transcription factors is not well described in the literature, in part because it is very difficult to study by mutagenesis. We used in vitro-binding techniques to identify characteristics of the ZAD family, by constructing glutathione Stransferase (GST)-ZAD domain chimeric proteins for use in protein binding assays, and GST-Zinc finger array domain chimera for binding site selections. Protein binding assays indicated a possible...
Show moreThe zinc finger associated domain (ZAD) containing family of transcription factors is not well described in the literature, in part because it is very difficult to study by mutagenesis. We used in vitro-binding techniques to identify characteristics of the ZAD family, by constructing glutathione Stransferase (GST)-ZAD domain chimeric proteins for use in protein binding assays, and GST-Zinc finger array domain chimera for binding site selections. Protein binding assays indicated a possible shared cofactor, as seen in the analogous KRAB system in mammals. DNA binding assays have provided a consensus binding sequence for five of the ZAD proteins, consistent with previously reported work on ZAD and unpublished work on mammalian transcription factors. Research is ongoing with an additional ~50 ZAD proteins to more fully map the binding characters of ZAD proteins.
Show less - Date Issued
- 2009
- PURL
- http://purl.flvc.org/FAU/186768
- Subject Headings
- Cellular signal transduction, Drosophila melanogaster, Cytogenetics, Transcription factors, Zinc-finger proteins, Synthesis, Genetic transcription, Regulation, Gene expression
- Format
- Document (PDF)
- Title
- Electroreception in the obligate freshwater stingray, Potamotrygon motoro.
- Creator
- Harris, Lindsay L., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Elasmobranch fishes use electroreception to detect electric fields in the environment, particularly minute bioelectric fields produced by potential prey. A single elasmobranch family (Potamotrygonidae) is composed of obligate freshwater stingrays endemic to the Amazon River. A freshwater existence has imposed morphological adaptions on their electrosensory system due to life in a high impedance medium. Because their electrosensory morphology differs from their marine relatives, freshwater...
Show moreElasmobranch fishes use electroreception to detect electric fields in the environment, particularly minute bioelectric fields produced by potential prey. A single elasmobranch family (Potamotrygonidae) is composed of obligate freshwater stingrays endemic to the Amazon River. A freshwater existence has imposed morphological adaptions on their electrosensory system due to life in a high impedance medium. Because their electrosensory morphology differs from their marine relatives, freshwater stingrays may demonstrate corresponding differences in behavioral sensitivity. The objective of this study was to quantify behavioral sensitivity of the obligate freshwater stingray Potamotrygon motoro to prey-simulating voltage. The voltage produced by common teleost prey of P. motoro were measured and replicated for behavioral trials. The best response was 10.62 cm, and the smallest voltage gradient detected was 0.005 mVcm-1. This sensitivity is reduced compared to marine species. The conductivity of the medium, more so than ampullary morphology, may dictate sensitivity of the elasmobranch electrosensory system.
Show less - Date Issued
- 2013
- PURL
- http://purl.flvc.org/fcla/dt/3362382
- Subject Headings
- Fishes, Sense organs, FIshes, Physiology, Stingrays, Physiology, Fish culture
- Format
- Document (PDF)
- Title
- Electrogenic metals for elasmobranch bycatch mitigation.
- Creator
- Smith, Kieran Thomas., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Commercial longline fishing results in large amounts of incidental bycatch of elasmobranch fishes (sharks, skates, and rays). Teleost species lack electrosensory systems and development of technologies which target the ampullary organs of sharks provides an avenue to selectively deter elasmobranchs without affecting the catch rate of target teleosts. Electric field measurements and a controlled scientific longline study were conducted testing whether the lanthanide metal neodymium or zinc...
Show moreCommercial longline fishing results in large amounts of incidental bycatch of elasmobranch fishes (sharks, skates, and rays). Teleost species lack electrosensory systems and development of technologies which target the ampullary organs of sharks provides an avenue to selectively deter elasmobranchs without affecting the catch rate of target teleosts. Electric field measurements and a controlled scientific longline study were conducted testing whether the lanthanide metal neodymium or zinc/graphite might reduce elasmobranch catch per unit effort (CPUE). Baited longline hooks were treated with neodymium and zinc/graphite and catch rates were compared to that of controls. Shark CPUE decreased by 60% on neodymium treated hooks and 80% on zinc/graphite treated hooks. The effectiveness of both treatments varied among species with significant reductions shown for Atlantic sharpnose sharks (Rhizoprionodon terranovae) but less dramatic differences for others. Zinc/graphite is potentially a viable tool for reduction of shark bycatch in a commercial longline fishery.
Show less - Date Issued
- 2013
- PURL
- http://purl.flvc.org/fcla/dt/3361053
- Subject Headings
- Fishery conservation, Sharks, Conservation, Lanthanide shift reagents, Wildlife management
- Format
- Document (PDF)
- Title
- Development of a novel assay for in vivo screening of neuromodulatory drugs and targeted disruption of cholinergic synaptic transmission in Drosophila melanogaster.
- Creator
- Mejia, Monica, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Finding novel compounds that affect neuronal or muscular function is of great interest, as they can serve as potential pharmacological agents for a variety of neurological disorders. For instance, conopeptides have been developed into powerful drugs like the painkiller PrialtTM. Most conopeptides, however, have yet to be characterized, revealing the need for a rapid and straightforward screening method. We have designed a novel bioassay, which allows for unbiased screening of biological...
Show moreFinding novel compounds that affect neuronal or muscular function is of great interest, as they can serve as potential pharmacological agents for a variety of neurological disorders. For instance, conopeptides have been developed into powerful drugs like the painkiller PrialtTM. Most conopeptides, however, have yet to be characterized, revealing the need for a rapid and straightforward screening method. We have designed a novel bioassay, which allows for unbiased screening of biological activity of compounds in vivo against numerous molecular targets on a wide variety of neurons and muscles in a rapid and straightforward manner. For this, we paired nanoinjection of compounds with electrophysiological recordings from the Giant Fiber System of Drosophila melanogaster, which mediates the escape response of the fly.
Show less - Date Issued
- 2013
- PURL
- http://purl.flvc.org/fcla/dt/3362560
- Subject Headings
- Drosophila melanogaster, Genetics, Drosophila melanogaster, Life cycles, Insects, Physiology, Developmental neurobiology, Neural transmission, Cholinergic mechanisms
- Format
- Document (PDF)
- Title
- Does size really matter: how synchrony and size affect the dynamic of aggression between two sympatric species of dolphin in the Bahamas.
- Creator
- Cuksick, Jessica A., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Bottlenose (Tursiops truncatus) and spotted (Stenella frontalis) dolphins are sympatric species, resident to Little Bahama Bank, Bahamas. A unique, dynamic methodology quantified how interspecific aggression changed over time in terms of the individuals participating, context, and behaviors used. The timing of human observation relative to the onset of aggression did not result in differences in the proportion of behaviors observed. Highly intense behaviors were used most often. The...
Show moreBottlenose (Tursiops truncatus) and spotted (Stenella frontalis) dolphins are sympatric species, resident to Little Bahama Bank, Bahamas. A unique, dynamic methodology quantified how interspecific aggression changed over time in terms of the individuals participating, context, and behaviors used. The timing of human observation relative to the onset of aggression did not result in differences in the proportion of behaviors observed. Highly intense behaviors were used most often. The synchronous state of spotted dolphin groups, not the presence alone, was a crucial factor in determining the onset and progression of aggression. When synchronous, spotted dolphins successfully dominated the larger bottlenose dolphins. Two levels of dominance were observed. Within a single encounter ("encounter level"), one species did dominate the other. When all aggressive encounters were considered collectively over the long term ("gross level"), one species did not dominate the other. The combination of contextual factors best determined the dynamic of interspecific aggression.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3342044
- Subject Headings
- Bottlenose dolphin, Behavior, Research, Atlantic spotted dolphin, Behavior, Research, Mammals, Behavior, Aggressive behavior in animals
- Format
- Document (PDF)