Current Search: Chen, Ying (x)
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Title
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Development of methods for the immobilization of enzymes used in 9,11-secosteroid biosynthesis.
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Creator
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Chen, Ying, Florida Atlantic University, Kerr, Russell G.
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Abstract/Description
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9,11-Secosteroids exhibit novel biological activity and interesting pharmacological properties. A biosynthetic study of 9,11-secogorgosterol and other novel 9,11-secosteroids using an enzyme extract from the gorgonian Pseudopterogorgia americana demonstrated that this system is an efficient catalyst for a wide range 9,11-secosteroid production. Two methods to recover the enzyme from 9,11-secosteroid synthesis have been developed: (1) membrane-enclosed enzymatic catalysis, which combines...
Show more9,11-Secosteroids exhibit novel biological activity and interesting pharmacological properties. A biosynthetic study of 9,11-secogorgosterol and other novel 9,11-secosteroids using an enzyme extract from the gorgonian Pseudopterogorgia americana demonstrated that this system is an efficient catalyst for a wide range 9,11-secosteroid production. Two methods to recover the enzyme from 9,11-secosteroid synthesis have been developed: (1) membrane-enclosed enzymatic catalysis, which combines certain of the advantages of soluble enzymes and immobilized enzymes, shows high activity, simple recovery and at least 5 cycles or 23 days; (2) covalently bonded enzyme-agarose gel, attached by cyanogen bromide via the amino groups of enzymes, show 80% activity of free enzymes and at least 7 cycles or 46 days. Based on stability and kinetic studies of these immobilized enzymes in 9,11-secosteroid synthesis, a continuous flow column reactor for scale-up of 9,11-secosteroid production has been developed with great efficiency, which represents the first enzymatic production of a bioactive compound from a marine invertebrate on a large scale.
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Date Issued
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1998
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PURL
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http://purl.flvc.org/fcla/dt/15521
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Subject Headings
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Octocorallia, Enzymes--Synthesis, Steroids
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Format
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Document (PDF)
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Title
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Three new peroxides from the sponge Plakinastrella species.
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Creator
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Chen, Ying, Killday, K. B., McCarthy, Peter J., Schimoler, Rebecca, Chilson, Katherine, Selitrennikoff, Claude, Pomponi, Shirley A., Wright, Amy E.
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Date Issued
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2001
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PURL
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http://purl.flvc.org/FCLA/DT/3319103
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Subject Headings
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Sponges --Research, Spectral analysis, Marine natural products, Peroxides, Antifungal agents
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Format
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Document (PDF)
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Title
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New Bioactive Peroxides from Marine Sponges of the Family Plakiniidae.
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Creator
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Chen, Ying, McCarthy, Peter J., Harmody, Dedra K., Schimoler, Rebecca, Chilson, Katherine, Selitrennikoff, Claude, Pomponi, Shirley A., Wright, Amy E.
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Date Issued
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2002
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PURL
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http://purl.flvc.org/FCLA/DT/3164099
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Subject Headings
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Sponges, Peroxides, Pathogenic fungi, Candida albicans, Aspergillus fumigatus
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Format
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Document (PDF)
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Title
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Lasonolides C—G, Five New Lasonolide Compounds from the Sponge Forcepia sp.
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Creator
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Wright, Amy E., Chen, Ying, Winder, Priscilla L., Pitts, Tara P., Pomponi, Shirley A., Longley, Ross E.
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Date Issued
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2004
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PURL
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http://purl.flvc.org/FCLA/DT/3164109
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Subject Headings
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Macrolide antibiotics, Natural products, Mass spectrometry, Sponges, Stereochemistry
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Format
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Document (PDF)