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DNA fingerprinting of human oral microbiome: a first step towards development of early diagnosis of oral diseases
Title
DNA fingerprinting of human oral microbiome: a first step towards development of early diagnosis of oral diseases.
Creator
Chakraborty, Shreyasee, Graduate College
Date Issued
2013-04-12
PURL
http://purl.flvc.org/fcla/dt/3361916
Subject Headings
DNA fingerprinting, Mouth--Diseases, Metagenomics
Format
Document (PDF)
DNA Fingerprints and ribotypes of the oral microbiome can distinguish smokers and oral disease patients from healthy non smokers.
Title
DNA Fingerprints and ribotypes of the oral microbiome can distinguish smokers and oral disease patients from healthy non smokers.
Creator
Chakraborty, Shreyasee, Persaud, Verneshia, Vergas, Sonya, Graduate College, Gautier, Gloryanne, Esiobu, Nwadiuto
Abstract/Description
Defining the bacterial communities associated with healthy status will permit rapid detection of shifts associated with disease and foster effective probiotic intervention. This study compares the PCR-RFLP of oral metagenomes and cultured bacterial community, as well as the bacterial diversity profile of smokers, non-smokers and oral disease patients. The goal was to evaluate the stability of bacteria associated with health and capture possible shifts potentially diagnostic of smokers and...
Show moreDefining the bacterial communities associated with healthy status will permit rapid detection of shifts associated with disease and foster effective probiotic intervention. This study compares the PCR-RFLP of oral metagenomes and cultured bacterial community, as well as the bacterial diversity profile of smokers, non-smokers and oral disease patients. The goal was to evaluate the stability of bacteria associated with health and capture possible shifts potentially diagnostic of smokers and oral disease. Oral wash samples were obtained from 5 healthy and 5 smokers twice, 3 months apart. Samples from 5 oral disease patients were also collected. Metagenomic and the genomic DNA of a cultured subset were amplified using primer 1492R and 27F.The generated 16SrRNA gene amplicons were used for arestriction digestion assay and bcloning with the Gene Hunter PCRTrap vector and pCR 4- TOPO cloning kits. The restriction fingerprints were statistically tested using Gel Compare II. The cloned 16S genes were sequenced using the ABI GeneAmp 9700 Thermal cycler. Sequences were analyzed by BLAST on the RDB II database and the HOMD. Sau 3AI and Alu I produced the best distinctive markers of 300 bp in healthy nonsmokers and 500bp in smokers in plate wash communities. The 16S sequence data suggest the presence of a core microbiota in all subjects mostly Streptococcus, Gamella, Candidatuse spp and confirmed that the smokers harbored highly diverse and distinct community Neisseria pharynges, Rothia mucilaginosa. Remarkably, there was a high stability of the fingerprints and diversity profile for smokers and nonsmokers after 3 months.
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Date Issued
2014
PURL
http://purl.flvc.org/fau/fd/FA00005808
Format
Document (PDF)
DNA fingerprints of human oral microbiome: a first step towards early diagnosis of oral diseases
Title
DNA fingerprints of human oral microbiome: a first step towards early diagnosis of oral diseases.
Creator
Chakraborty, Shreyasee, Esiobu, Nwadiuto, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
This study evaluated the stability of oral bacteria in healthy subjects and documented community shifts in smokers and oral/periodontal disease by employing PCR-RFLP, DGGE and sequence analysis of the 16S rDNA gene from metagenomes and plate-wash (cultured) bacteria of oral wash from 15 participants,. A stable core of bacterial DNA fingerprint was detected within and between subjects and did not change over time when analyzed in smokers and healthy non-smokers. Signature bands in smokers, non...
Show moreThis study evaluated the stability of oral bacteria in healthy subjects and documented community shifts in smokers and oral/periodontal disease by employing PCR-RFLP, DGGE and sequence analysis of the 16S rDNA gene from metagenomes and plate-wash (cultured) bacteria of oral wash from 15 participants,. A stable core of bacterial DNA fingerprint was detected within and between subjects and did not change over time when analyzed in smokers and healthy non-smokers. Signature bands in smokers, non-smokers and periodontal disease subjects were evident suggesting the presence of potential indicators of health and poor oral health. Taxon diversity was higher in smokers including members of the genera Rothia, Synechococcus, Neisseria, Thiomargarita and Pyrobaculum but highest in periodontal disease. The two techniques successfully aligned the subjects within appropriate categories (based on their oral microbial genetic patterns)confirming their diagnostic suitability.
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Date Issued
2014
PURL
http://purl.flvc.org/fau/fd/FA00004184, http://purl.flvc.org/fau/fd/FA00004184
Subject Headings
Molecular microbiology., Mouth--Microbiology., Bacterial genetics., Cellular signal transduction., Microbial genomics.
Format
Document (PDF)