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Alkaline phosphatase activity in symbiotic dinoflagellates (zooxanthellae) as a biological indicator of environmental phosphate exposure
Title
Alkaline phosphatase activity in symbiotic dinoflagellates (zooxanthellae) as a biological indicator of environmental phosphate exposure.
Creator
Annis, E. R., Cook, Clayton B.
Date Issued
2002
PURL
http://purl.flvc.org/FCLA/DT/3350894
Subject Headings
Dinoflagellates, Zooxanthella, Symbiosis, Alkaline phosphatase, Acid phosphatase, Montastraea
Format
Document (PDF)
The influence of nearshore waters on corals of the Florida reef tract.
Title
The influence of nearshore waters on corals of the Florida reef tract.
Creator
Cook, Clayton B., Mueller, E. M., Ferrier, M. D., Annis, E. R.
Date Issued
2002
PURL
http://purl.flvc.org/fau/fd/FA00007396
Subject Headings
Florida, Straits of, Reefs--Florida, Corals--Florida, Montastraea, Corals--Growth
Format
Document (PDF)
Comprehensive health assessment and blood analyte reference intervals of gopher tortoises (Gopherus polyphemus) in southeastern FL, USA
Title
Comprehensive health assessment and blood analyte reference intervals of gopher tortoises (Gopherus polyphemus) in southeastern FL, USA.
Creator
Annie Page-Karjian, Kathleen Rafferty, Clerson Xavier, Nicole I. Stacy, Jon A. Moore, Sarah E. Hirsch, Samantha Clark, Charles A. Manire, Justin R. Perrault
Abstract/Description
The gopher tortoise (Gopherus polyphemus), a keystone species, is declining throughout its geographic range. Lack of knowledge with respect to the potential infectious diseases present within wild populations creates a dilemma for wildlife biologists, conservationists and public policy makers. The objective of this study was to conduct a health assessment of two previously unstudied gopher tortoise aggregations located at two sites in southeastern FL. Samples were collected from 91 tortoises ...
Show moreThe gopher tortoise (Gopherus polyphemus), a keystone species, is declining throughout its geographic range. Lack of knowledge with respect to the potential infectious diseases present within wild populations creates a dilemma for wildlife biologists, conservationists and public policy makers. The objective of this study was to conduct a health assessment of two previously unstudied gopher tortoise aggregations located at two sites in southeastern FL. Samples were collected from 91 tortoises (48 adults, 35 juveniles, 8 hatchlings) captured at Florida Atlantic University’s Harbor Branch Oceanographic Institute, in Fort Pierce, FL, USA in 2019, and Loggerhead Park in Juno Beach, FL, USA, during 2018–2019. Samples of blood, nasal swabs and oral/cloacal swabs were analyzed for hematology, plasma protein electrophoretic profiles and infectious disease testing including Mycoplasma spp. serology and polymerase chain reaction (PCR) assays for Ranavirus, Herpesvirus and Anaplasma spp. Hematological and plasma protein electrophoresis reference intervals are presented for adult and juvenile tortoises from both sites combined. Clinical signs consistent with upper respiratory tract disease (URTD) were observed in 18/91 (20%) tortoises, and antibodies to Mycoplasma agassizii were detected in 33/77 (42.9%) tortoises. Adult tortoises were significantly more likely than juveniles to have URTD clinical signs, and statistically significant, positive relationships were observed between the presence of antibodies to Mycoplasma spp. and carapace length, packed cell volume and plasma globulin concentrations. Anaplasma spp. inclusions were observed in 8/82 (10%) tortoises, but PCR detected Anaplasma sp. in 21/83 (25%) tortoises. Herpesvirus and Ranavirus were not detected in any blood or swab samples. This work contributes important baseline information on the health of gopher tortoises toward the southern end of the species’ range.
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Date Issued
2021
PURL
http://purl.flvc.org/fau/fd/FAUIR000554
Format
Document (PDF)
Chelonid Alphaherpesvirus 5 Prevalence and First Confirmed Case of Sea Turtle Fibropapillomatosis in Grenada, West Indies
Title
Chelonid Alphaherpesvirus 5 Prevalence and First Confirmed Case of Sea Turtle Fibropapillomatosis in Grenada, West Indies.
Creator
Amanda James, Annie Page-Karjian, Kate E. Charles, Jonnel Edwards, Christopher R. Gregory, Sonia Cheetham, Brian P. Buter, David P. Marancik
Abstract/Description
Chelonid alphaherpesvirus 5 (ChHV5) is strongly associated with fibropapillomatosis, a neoplastic disease of sea turtles that can result in debilitation and mortality. The objectives of this study were to examine green (Chelonia mydas), hawksbill (Eretmochelys imbricata), and leatherback (Dermochelys coriacea) sea turtles in Grenada, West Indies, for fibropapillomatosis and to utilize ChHV5-specific PCR, degenerate herpesvirus PCR, and serology to non-invasively evaluate the prevalence of...
Show moreChelonid alphaherpesvirus 5 (ChHV5) is strongly associated with fibropapillomatosis, a neoplastic disease of sea turtles that can result in debilitation and mortality. The objectives of this study were to examine green (Chelonia mydas), hawksbill (Eretmochelys imbricata), and leatherback (Dermochelys coriacea) sea turtles in Grenada, West Indies, for fibropapillomatosis and to utilize ChHV5-specific PCR, degenerate herpesvirus PCR, and serology to non-invasively evaluate the prevalence of ChHV5 infection and exposure. One-hundred and sixty-seven turtles examined from 2017 to 2019 demonstrated no external fibropapilloma-like lesions and no amplification of ChHV5 DNA from whole blood or skin biopsies. An ELISA performed on serum detected ChHV5-specific IgY in 18/52 (34.6%) of green turtles tested. In 2020, an adult, female green turtle presented for necropsy from the inshore waters of Grenada with severe emaciation and cutaneous fibropapillomas. Multiple tumors tested positive for ChHV5 by qPCR, providing the first confirmed case of ChHV5-associated fibropapillomatosis in Grenada. These results indicate that active ChHV5 infection is rare, although viral exposure in green sea turtles is relatively high. The impact of fibropapillomatosis in Grenada is suggested to be low at the present time and further studies comparing host genetics and immunologic factors, as well as examination into extrinsic factors that may influence disease, are warranted.
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Date Issued
2021
PURL
http://purl.flvc.org/fau/fd/FAUIR000553
Format
Document (PDF)
Molecular Assessment of Chelonid Alphaherpesvirus 5 Infection in Tumor-Free Green (Chelonia mydas) and Loggerhead (Caretta caretta) Sea Turtles in North Carolina, USA, 2015–2019
Title
Molecular Assessment of Chelonid Alphaherpesvirus 5 Infection in Tumor-Free Green (Chelonia mydas) and Loggerhead (Caretta caretta) Sea Turtles in North Carolina, USA, 2015–2019.
Creator
Annie Page-Karjian, Maria E. Serrano, Jeffrey Cartzendafner, Ashley Morgan, Branson W. Ritchie, Christopher R. Gregory, Joanne Braun McNeill, Justin R. Perrault, Emily F. Christiansen, Craig A. Harms
Abstract/Description
Fibropapillomatosis is a debilitating tumor disease of sea turtles that is sometimes fatal. This disease is a key concern for sea turtle rehabilitation facilities due to its infectious nature, as it is associated with a virus called chelonid alphaherpesvirus 5. This is the first study to analyze antibodies to this virus in loggerhead sea turtles and represents the most complete dataset on viral detection in sea turtles encountered in the more northern latitudes of their habitat in the western...
Show moreFibropapillomatosis is a debilitating tumor disease of sea turtles that is sometimes fatal. This disease is a key concern for sea turtle rehabilitation facilities due to its infectious nature, as it is associated with a virus called chelonid alphaherpesvirus 5. This is the first study to analyze antibodies to this virus in loggerhead sea turtles and represents the most complete dataset on viral detection in sea turtles encountered in the more northern latitudes of their habitat in the western Atlantic.
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Date Issued
2020
PURL
http://purl.flvc.org/fau/fd/FAUIR000556
Format
Document (PDF)
Insights on Immune Function in Free-Ranging Green Sea Turtles (Chelonia mydas) with and without Fibropapillomatosis
Title
Insights on Immune Function in Free-Ranging Green Sea Turtles (Chelonia mydas) with and without Fibropapillomatosis.
Creator
Justin R. Perrault, Milton Levin, Cody R. Mott, Caitlin M. Bovery, Michael J. Bresette, Ryan M. Chabot, Christopher R. Gregory, Jeffrey R. Guertin, Sarah E. Hirsch, Branson W. Ritchie, Steven T. Weege, Ryan C. Welsh, Blair E. Witherington, Annie Page-Karjian
Abstract/Description
Chelonid alphaherpesviruses 5 and 6 (ChHV5 and ChHV6) are viruses that affect wild sea turtle populations. ChHV5 is associated with the neoplastic disease fibropapillomatosis (FP), which affects green turtles (Chelonia mydas) in panzootic proportions. ChHV6 infection is associated with lung-eye-trachea disease (LETD), which has only been observed in maricultured sea turtles, although antibodies to ChHV6 have been detected in free-ranging turtles. To better understand herpesvirus prevalence...
Show moreChelonid alphaherpesviruses 5 and 6 (ChHV5 and ChHV6) are viruses that affect wild sea turtle populations. ChHV5 is associated with the neoplastic disease fibropapillomatosis (FP), which affects green turtles (Chelonia mydas) in panzootic proportions. ChHV6 infection is associated with lung-eye-trachea disease (LETD), which has only been observed in maricultured sea turtles, although antibodies to ChHV6 have been detected in free-ranging turtles. To better understand herpesvirus prevalence and host immunity in various green turtle foraging aggregations in Florida, USA, our objectives were to compare measures of innate and adaptive immune function in relation to (1) FP tumor presence and severity, and (2) ChHV5 and ChHV6 infection status. Free-ranging, juvenile green turtles (N = 45) were captured and examined for external FP tumors in Florida’s Big Bend, Indian River Lagoon, and LakeWorth Lagoon. Blood samples were collected upon capture and analyzed for ChHV5 and ChHV6 DNA, antibodies to ChHV5 and ChHV6, in vitro lymphocyte proliferation using a T-cell mitogen (concanavalin A), and natural killer cell activity. Despite an overall high FP prevalence (56%), ChHV5 DNA was only observed in one individual, whereas 20% of turtles tested positive for antibodies to ChHV5. ChHV6 DNA was not observed in any animals and only one turtle tested positive for ChHV6 antibodies. T-cell proliferation was not significantly related to FP presence, tumor burden, or ChHV5 seroprevalence; however, lymphocyte proliferation in response to concanavalin A was decreased in turtles with severe FP (N = 3). Lastly, green turtles with FP (N = 9) had significantly lower natural killer cell activity compared to FP-free turtles (N = 5). These results increase our understanding of immune system effects related to FP and provide evidence that immunosuppression occurs after the onset of FP disease.
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Date Issued
2021
PURL
http://purl.flvc.org/fau/fd/FAUIR000555
Format
Document (PDF)
Efficient laser pulse dispersion codes for turbid undersea imaging and communications applications
Title
Efficient laser pulse dispersion codes for turbid undersea imaging and communications applications.
Creator
Dalgleish, Fraser R., Caimi, F. M., Vuorenkoski, Anni K., Britton, W. B., Ramos, Brian, Giddings, T. E., Shirron, J. J., Mazel, C. H.
Date Issued
2010
PURL
http://purl.flvc.org/FCLA/DT/3340792
Subject Headings
Underwater imaging systems, Laser communication systems, Radiative transfer, Monte Carlo method
Format
Document (PDF)
Evaluating prevalence of external injuries on nesting loggerhead sea turtles Caretta caretta in southeastern Florida, USA
Title
Evaluating prevalence of external injuries on nesting loggerhead sea turtles Caretta caretta in southeastern Florida, USA.
Creator
Aleah Ataman, Alison M. Gainsbury, Charles A. Manire, Sarah L. Hoffmann, Annie Page-Karjian, Sarah E. Hirsch, Maximilian M. R. Polyak, Deby L. Cassill, Derek M. Aoki, Katelyn M. Fraser, Skyler Klingshirn, Jamie A. Stoll, Justin R. Perrault
Abstract/Description
Sea turtles face both anthropogenic and natural threats including boat strikes, fisheries, pollution, and predator attacks. Injuries from anthropogenic sources are more common than naturally caused injuries. The goal of this study was to determine prevalence and cause (e.g. boat strike, entanglement, hook, shark bite) of injuries on nesting loggerhead sea turtles Caretta caretta on Juno and Jupiter beaches, Florida, USA. During the 2019 and 2020 nesting seasons, 450 loggerhead females were...
Show moreSea turtles face both anthropogenic and natural threats including boat strikes, fisheries, pollution, and predator attacks. Injuries from anthropogenic sources are more common than naturally caused injuries. The goal of this study was to determine prevalence and cause (e.g. boat strike, entanglement, hook, shark bite) of injuries on nesting loggerhead sea turtles Caretta caretta on Juno and Jupiter beaches, Florida, USA. During the 2019 and 2020 nesting seasons, 450 loggerhead females were examined for external injuries. Injuries were categorized by anatomic location, condition, and cause. We found that 24% of loggerheads had at least 1 injury. Of the 111 injuries found on 107 nesting females, 88% were healed, 9% were partially healed with some scarred tissue, and 3% were fresh injuries. Most injuries (55%) were lateral injuries on the carapace or appendages. We were able to attribute 60 injuries to a specific cause. Boat strikes accounted for 75% of the 60 injuries, shark bites accounted for 15%, fishing hooks accounted for 7%, and entanglements accounted for the remaining 3%. This study provides new insight into the prevalence of anthropogenic injuries relative to natural injuries in loggerhead sea turtles nesting in the most densely nested beach in the Western Hemisphere and can be used to improve conservation management plans through implementation of fishing and/or boating restrictions in the nesting and foraging areas most commonly frequented by sea turtles.
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Date Issued
2021
PURL
http://purl.flvc.org/fau/fd/FAUIR000551
Format
Document (PDF)
Underwater laser serial imaging using compressive sensing and digital mirror device
Title
Underwater laser serial imaging using compressive sensing and digital mirror device.
Creator
Ouyang, Bing, Dalgleish, Fraser R., Caimi, F. M., Giddings, T. E., Shirron, J. J., Vuorenkoski, Anni K., Nootz, G., Britton, W. B., Ramos, Brian
Date Issued
2011
PURL
http://purl.flvc.org/FCLA/DT/3340793
Subject Headings
Underwater imaging systems, Image compression, Lasers, Signal processing
Format
Document (PDF)