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Title: EFFECTS OF NATURAL AND ANTHROPOGENIC IMPACTS ON THE MICROBIOME OF THE INDIAN RIVER LAGOON, FL, USA.
Creator: Bradshaw II, David J., McCarthy, Peter J., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: The Indian River Lagoon (IRL) FL, USA, is an Estuary of National Significance due to its economic and high biodiversity. Microbial populations are understudied in the IRL despite their numerous ecological services. A two-year, nineteen-site Lagoon-Wide Survey (LWS) was conducted to provide the first 16S rRNA amplicon sequencing data on the microbiome of the sediment in the IRL and determine how the microbiome changed in response to environmental and anthropogenic factors. The most influential...
Show moreThe Indian River Lagoon (IRL) FL, USA, is an Estuary of National Significance due to its economic and high biodiversity. Microbial populations are understudied in the IRL despite their numerous ecological services. A two-year, nineteen-site Lagoon-Wide Survey (LWS) was conducted to provide the first 16S rRNA amplicon sequencing data on the microbiome of the sediment in the IRL and determine how the microbiome changed in response to environmental and anthropogenic factors. The most influential variables that explained the variability between microbiomes were porewater salinity, total organic matter (TOM), and copper (Cu). These results correlated with some of the anthropogenic pressures the IRL faces such as freshwater discharges from St. Lucie Estuary (SLE), trace metal contamination, and the accumulation of fine-grained, highly organic sediment known as “IRL muck” (muck). Research then focused on determining the microbial differences between three sets of sample types: sediment from the IRL versus the SLE; sediment that had three muck characteristics versus those with zero; and high TOM sediment that had high Cu versus high TOM sediment that had low Cu. Differentially abundant prokaryotes between sample types were determined with novel indicator analysis techniques. One technique tested the effectiveness of an indicator list to separate samples based upon the product of the sensitivity and specificity of partitioning around medoids clustering in comparison to metadata classifications. The other technique allowed for the tracking of changes in the entire indicator microbiome. These new indicator analysis techniques were created using the original LWS data and tested to determine how sediment microbiomes responded during two opportunistic surveys: dredging of muck from an IRL tributary (Eau Gallie River) and Hurricane Irma. These studies have filled the knowledge gap regarding the unknown microbiome of the IRL and how sediment microbiomes respond to extreme events such as dredging and a hurricane. They also led to the development of new indicator analysis techniques that can be used by to track changes in the entire indicator microbiome.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013446
Subject Headings: Indian River (Fla. : Lagoon), Microbiota, Anthropogenic effects on nature, Estuarine ecology
Format: Document (PDF)

Title: Novel Detection Techniques for Viable but Nonculturable Vibrio Vulnificus Cells in Response to Elevated Salinity.
Creator: McHenry, Brandon, McCarthy, Peter J., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Vibrio vulnificus is a marine pathogen of human health concern, capable of causing potentially fatal wound infections in a select group of the population. Previous studies have indicated this species’ strong negative correlation with salinity, not typically found above 30 ppt. This study assessed the ability of V. vulnificus to become Viable But Nonculturable in response to elevated salinity (35 ppt) as well as investigated novel methods for confirming their entrance into this state. Results...
Show moreVibrio vulnificus is a marine pathogen of human health concern, capable of causing potentially fatal wound infections in a select group of the population. Previous studies have indicated this species’ strong negative correlation with salinity, not typically found above 30 ppt. This study assessed the ability of V. vulnificus to become Viable But Nonculturable in response to elevated salinity (35 ppt) as well as investigated novel methods for confirming their entrance into this state. Results showed a complete loss of culturability in both Environmental and Clinical strains of this bacterium by 9 days after inoculation. Using a High Content Imager, it was determined that these pathogens were not dying (< 10%) in response to the treatment and were partially becoming cocci (≈35%). This study indicates the importance of understanding the impact environmental parameters have on this human pathogen, and what it means for reliably detecting them.
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Date Issued: 2019
PURL: http://purl.flvc.org/fau/fd/FA00013240
Subject Headings: Vibrio vulnificus, Pathogenic microorganisms--Detection, Salinity
Format: Document (PDF)

Title: An Assessment of Fecal Pollution in the St. Lucie Estuary and Indian River Lagoon.
Creator: Perricone, Carlie S., McCarthy, Peter J., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Contamination of recreational waters with fecal waste, indicated by the presence of enterococci, can have consequences for human and ecosystem health. The difficulty in determining the extent and origin of fecal pollution in dynamic estuarine systems is compounded by contributions from sources including septic tanks and agricultural runoff. This study investigated fecal pollution at five sites with variable hydrological conditions in the St. Lucie Estuary and Indian River Lagoon. The...
Show moreContamination of recreational waters with fecal waste, indicated by the presence of enterococci, can have consequences for human and ecosystem health. The difficulty in determining the extent and origin of fecal pollution in dynamic estuarine systems is compounded by contributions from sources including septic tanks and agricultural runoff. This study investigated fecal pollution at five sites with variable hydrological conditions in the St. Lucie Estuary and Indian River Lagoon. The distribution and occurrence of enterococci was assessed using traditional cultivation. A human-associated microbial source tracking assay was validated and applied in these estuaries using qPCR. Results demonstrated a correlation between enterococci concentrations, rainfall, and salinity. The human-associated assay was sensitive and specific in the lab; however, human fecal pollution was not detected in the field even though samples contained high levels of enterococci. This study highlights the importance of expanding the range of water quality assessment.
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Date Issued: 2017
PURL: http://purl.flvc.org/fau/fd/FA00005023, http://purl.flvc.org/fau/fd/FA00005932
Subject Headings: Dissertations, Academic -- Florida Atlantic University, Indian River (Fla. : Lagoon), Saint Lucie River Estuary (Fla.), Enterococcus., Fecal matter., Estuarine pollution.
Format: Document (PDF)

Title: Florida Sea Grant: Frequently Asked Questions about Vibrio in Florida.
Creator: Holly Abeels, Gabby Barbarite, Anita Wright, Peter J. McCarthy
Abstract/Description: Under certain conditions, one type of marine bacteria known as Vibrio is capable of causing wound infections and seafood sickness in people with weakened immune systems. Recently, the term “flesh-eating bacteria” has been used to refer to Vibrio. This description is false and misleading and causes unnecessary fear and panic. To ensure that your time on the water is safe and enjoyable, be aware of local hazards and follow these simple safety guidelines.
Date Issued: 2016-02
PURL: http://purl.flvc.org/fau/fd/FAUIR000479
Format: Document (PDF)

Title: The Occurrence of Vibrio vulnificus, V. parahaemolyticus and V. cholerae in the Indian River Lagoon, Florida, with Implications for Human Health.
Creator: Barbarite, Gabrielle M., McCarthy, Peter J., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Vibrio bacteria are emerging pathogens responsible for 80,000 illnesses and 100 deaths in the United States each year. Infections are directly linked to the marine environment and are acquired by consuming contaminated seafood or exposing wounds during aquatic activities. Florida has the highest national incidence of vibriosis, with 20% of its cases reported from the Indian River Lagoon region, a popular recreation destination. This study utilized a combination of cultivation and molecular...
Show moreVibrio bacteria are emerging pathogens responsible for 80,000 illnesses and 100 deaths in the United States each year. Infections are directly linked to the marine environment and are acquired by consuming contaminated seafood or exposing wounds during aquatic activities. Florida has the highest national incidence of vibriosis, with 20% of its cases reported from the Indian River Lagoon region, a popular recreation destination. This study utilized a combination of cultivation and molecular techniques to investigate the local distribution of V. vulnificus, V. parahaemolyticus and V. cholerae in this local waterway. The targeted species were found in an array of samples which may facilitate their transmission to humans. Overall, these bacteria were abundant in estuarine sediments (Vp: 2,439 CFU/g, Vv: 303 CFU/g, Vc: 176 CFU/g), on the sharp edges of oyster shells (Vp: 82 CFU/cm, Vv: 102 CFU/cm, Vc: 41 CFU/cm), and in the water column (Vp: 3.78 CFU/ml, Vv: 5.51 CFU/ml, Vc: 2.46 CFU/ml). Vibrio also pose a hazard to recreational anglers as they were recovered from fish (Vp: 61%, Vv: 55%, Vc: 30%), live bait shrimp (Vp: 80%, Vv: 37%, Vc: 0%) and hooks (Vp: 32%, Vv: 18%, Vc: 0%). Additionally, a molecular analysis of the V. vulnificus virulence revealed that the local population was dominated by disease-causing (vcgC) strains, which may explain why wound-related infections are common in this region. Vibrio occurrence varied both spatially and temporally due to their relationship with salinity and temperature. These bacteria exhibited a strong negative correlation with salinity, being particularly abundant near freshwater discharge locations. Due to Florida’s year-round warm climate, these species were found to be permanent members of the local microbial community. Seasonal peaks in abundance occurred between August and October, a period which corresponds with the warmest water temperatures as well as frequent rainfall. Predictive models were constructed based on these parameters to provide a better understanding of how, when and where Vibrio spp. may be encountered by humans. This information is important for both water management and healthcare initiatives, with an overall goal of improving local recreational safety.
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Date Issued: 2016
PURL: http://purl.flvc.org/fau/fd/FA00004705
Subject Headings: Health risk assessment, Marine microbiology, Pathogenic microorganisms, Vibrio infections, Vibrionaceae
Format: Document (PDF)

Title: Frequently Asked Questions about Vibrio in Florida.
Creator: Gabby Barbarite, Peter J McCarthy, Holly Abeels, Anita Wright
Abstract/Description: Florida is a tropical paradise that attracts marine enthusiasts and seafood lovers from around the world. Its extensive waterways not only offer unique areas for us to explore but also provide essential habitat for marine life. Although you cannot see them, marine bacteria are common throughout Florida waters. Marine bacteria play an important role in environmental processes by breaking down organic matter or providing food for larger organisms. There are many different species of bacteria...
Show moreFlorida is a tropical paradise that attracts marine enthusiasts and seafood lovers from around the world. Its extensive waterways not only offer unique areas for us to explore but also provide essential habitat for marine life. Although you cannot see them, marine bacteria are common throughout Florida waters. Marine bacteria play an important role in environmental processes by breaking down organic matter or providing food for larger organisms. There are many different species of bacteria that live in Florida waters. They are natural inhabitants of marine environments and live in and on many aquatic organisms. Under certain conditions, one type of marine bacteria known as Vibrio is capable of causing wound infections and seafood sickness in people with weakened immune systems.
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Date Issued: 2015-11
PURL: http://purl.flvc.org/fau/fd/FAUIR000480
Format: Document (PDF)

Title: Two distinct microbial communities revealed in the sponge Cinachyrella.
Creator: Cuvelier, Marie L., Blake, Emily, Mulheron, Rebecca, McCarthy, Peter J., Blackwelder, Patricia, Thurber, Rebecca L. Vega, Lopez, Jose V.
Date Issued: 2014-11-04
PURL: http://purl.flvc.org/fau/fd/FAUIR000172
Format: Citation

Title: Bacillus strains most closely-related to Bacillus nealsonii are not effectively circumscribed within the taxonomic species definition.
Creator: McCarthy, Peter J., Peak, K. K., Duncan, K. E., Luna, V. A., King, D. S., Cannons, A. C
Date Issued: 2011
PURL: http://purl.flvc.org/FCLA/DT/3318913
Subject Headings: Bacillus (Bacteria), Bacillus (Bacteria) --Classification, Bacteria--Classification--Technique, Sporeforming bacteria
Format: Document (PDF)

Title: Population dynamics of Vibrio spp. associated with marine sponge microcosms.
Creator: Hoffmann, M., Fischer, M., Ottesen, A., McCarthy, Peter J., Lopez, Jose V., Brown, E. W., Monday, S. R.
Date Issued: 2010
PURL: http://purl.flvc.org/FCLA/DT/3351984
Subject Headings: Sponges, Vibrio, Microbial ecology, RNA, Ribosomal, 16S
Format: Document (PDF)

Title: Gymnochromes E and F, cytotoxic phenanthroperylenequinones from a deep-water crinoid, Holopus rangii.
Creator: Wangun, H. V. K., Wood, A., Fiorilla, C., Reed, John K., McCarthy, Peter J., Wright, Amy E.
Date Issued: 2010
PURL: http://purl.flvc.org/FCLA/DT/3319112
Subject Headings: Crinoidea, Marine natural products, Cytotoxin, Marine metabolites, Anthraquinones
Format: Document (PDF)

Title: Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy sediments in the Straits of Florida.
Creator: Bruck, W. M., Bruck, T. B., Self, W. T., Reed, John K., Nitecki, S. S., McCarthy, Peter J.
Date Issued: 2010
PURL: http://purl.flvc.org/FCLA/DT/3351982
Subject Headings: Microbial ecology, Marine sediments, Florida, Straits of, Fluorescence in situ hybridization, Sponges
Format: Document (PDF)

Title: Isolation, synthesis, and biological activity of Aphrocallistin, an adenine-substituted bromotyramine metabolite from the hexactinellida sponge Aphrocallistes beatrix.
Creator: Wright, Amy E., Roth, G. P., Hoffman, J. K., Divlianska, D. B., Pechter, D., Sennett, Susan H., Guzman, Esther A., Linley, P. A., McCarthy, Peter J., Pitts, Tara P., Pomponi, Shirley A., Reed, John K.
Date Issued: 2009
PURL: http://purl.flvc.org/FCLA/DT/3319109
Subject Headings: Sponges --Research, Marine metabolites, Marine natural products, Hexactinellida
Format: Document (PDF)

Title: The many faces of gene expression profiling: transcriptome analyses applied towardselucidating marine organismal interactions and metabolism.
Creator: Lopez, Jose V., Mouzon, B., McCarthy, Peter J., Kerr, Russell G.
Date Issued: 2009
PURL: http://purl.flvc.org/fau/fd/FA00007286
Subject Headings: Gene expression, Transcriptome, Molecular biotechnology, Marine ecology, Marine organisms
Format: Document (PDF)

Title: Use of real-time qPCR to quantify members of the unculturable heterotrophic bacterial community in a deep sea marine sponge, Vetulina sp.
Creator: Cassler, M., Peterson, C. L., Ledger, Angela, Pomponi, Shirley A., Wright, Amy E., Winegar, R., McCarthy, Peter J., Lopez, Jose V.
Date Issued: 2008
PURL: http://purl.flvc.org/FCLA/DT/2708323
Subject Headings: Microbiology --Research, Sponges --Microbiology, Marine biology --Caribbean Area
Format: Document (PDF)

Title: Comparison of bacterial diversity within the coral reef sponge, Axinella corrugata, the encrusting coral Erythropodium caribaeorum.
Creator: Lopez, Jose V., Ranzer, L. K., Ledger, Angela, Schoch, B., Duckworth, Alan R., McCarthy, Peter J., Kerr, Russell G.
Date Issued: 2008
PURL: http://purl.flvc.org/fau/fd/FA00007036
Subject Headings: Sponges, Corals, Heterotrophic bacteria, Symbiosis, RNA, Ribosomal, 16S
Format: Document (PDF)

Title: Identification of the bacterial symbiont Entotheonella sp. in the mesohyl of the marine sponge Discodermia sp.
Creator: Bruck, W. M., Sennett, Susan H., Pomponi, Shirley A., Willenz, P., McCarthy, Peter J.
Date Issued: 2008
PURL: http://purl.flvc.org/FCLA/DT/3183806
Subject Headings: Bacteria Ecology, Symbiosis, Sponges
Format: Document (PDF)

Title: Neopeltolide a new macrolide from a lithistid sponge of the family Neopeltidae.
Creator: Wright, Amy E., Botelho, J. C., Guzman, Esther A., Harmody, Dedra K., Linley, P. A., McCarthy, Peter J., Pitts, Tara P., Pomponi, Shirley A., Reed, John K.
Date Issued: 2007
PURL: http://purl.flvc.org/FCLA/DT/3333011
Subject Headings: Sponges, Macrolides, Marine natural products
Format: Document (PDF)

Title: The diversity of the bacterial communities associated with the azooxanthellate hexacoral Cirrhipathes lutkeni.
Creator: Santiago-Vázquez, L. Z., Brück, T. B., Bruck, W. M., Duque-Alarcón, A. P., McCarthy, Peter J., Kerr, Russell G.
Date Issued: 2007
PURL: http://purl.flvc.org/FCLA/DT/3183801
Subject Headings: Symbiosis, Hexacorals, Bacterial diversity, Marine bacteria
Format: Document (PDF)

Title: Tsukamurella spongiae sp. nov., a novel actinomycete isolated from a deep-water marinesponge.
Creator: Olson, Julie B., Harmody, Dedra K., Bej, A. K., McCarthy, Peter J.
Date Issued: 2007
PURL: http://purl.flvc.org/fau/fd/FA00007117
Subject Headings: Curaçao, Sponges, Actinobacteria, Actinomycetes, RNA, Ribosomal, 16S
Format: Document (PDF)

Title: Diversity of the Bacterial Communities Associated with the Azooxanthellate Deep Water Octocorals Leptogorgia minimata, Iciligorgia schrammi, and Swiftia exertia, Marine Biotechnology.
Creator: Brück, Thomas B., Brück, Wolfram M., Santiago-Vázquez, Lory Z., McCarthy, Peter J., Kerr, Russell G.
Date Issued: 2007
PURL: http://purl.flvc.org/FCLA/DT/2796098
Subject Headings: Octocorallia, Microbial ecology, Microorganisms --United States --Identification, Marine microbiology, Pompano Beach (Fla.)
Format: Document (PDF)

HBOI Faculty Contributions (50)	+ -
FAU Theses and Dissertations (4)	+ -
FAU College Collections (1)	+ -

Sponges (24)	+ -
Marine natural products (18)	+ -
Antifungal agents (8)	+ -
Marine metabolites (6)	+ -
Sponges --Research (6)	+ -

Bioactive compounds (5)	+ -
Symbiosis (5)	+ -
Marine microbiology (4)	+ -
Microbial ecology (4)	+ -
Candida albicans (3)	+ -
Marine organisms (3)	+ -
Microorganisms (3)	+ -
Peroxides (3)	+ -
RNA, Ribosomal, 16S (3)	+ -
Alkaloids (2)	+ -
Aspergillus fumigatus (2)	+ -
Cytotoxin (2)	+ -
Demospongiae (2)	+ -
Enzyme inhibitors (2)	+ -
Heterotrophic bacteria (2)	+ -
Indian River (Fla. : Lagoon) (2)	+ -
Indole alkaloids (2)	+ -
Marine invertebrates (2)	+ -
Marine sediments (2)	+ -
Peptides (2)	+ -
Porifera (2)	+ -
Sesquiterpenes (2)	+ -
Actinobacteria (1)	+ -
Actinomycetes (1)	+ -
Anthraquinones (1)	+ -

article (48)	+ -
Electronic Thesis or Dissertation (4)	+ -
poster (1)	+ -

McCarthy, Peter J. (52)	+ -
Pomponi, Shirley A. (24)	+ -
Wright, Amy E. (21)	+ -
Lopez, Jose V. (10)	+ -
Gunasekera, Sarath P. (9)	+ -

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