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Identification and characterization of a newly discovered reovirus strain

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Date Issued:
2000
Summary:
In this study, we report the identification and characterization of a new Reovirus strain isolated from the spinal fluid of a human patient. Unlike other reovirus serotypes that can be isolated from fecal matter, this strain was isolated from the spinal fluid of a patient; which suggests it may be a more pathogenic strain. The new isolate was compared to the three known strains of mammalian reoviruses. We have found that the virus is not a reassortment of the other three serotypes. This was confirmed by examination of the migration rates of the genomic dsRNA segments of all four viruses. The new isolate demonstrated measurable differences in viral protein molecular weights, when compared to the proteins of the three known serotypes. The molecular weight of the sigma I protein of the new isolate, was found to be more then 3Kd smaller then sigma 1 obtained from the other three serotypes. The largest difference in protein molecular weights was found with mu1C, which was about 7Kd smaller. The growth rate of the new virus was very similar to that of the other three serotypes. Some differences were found in select cell lines.
Title: Identification and characterization of a newly discovered reovirus strain.
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Name(s): Jerusalmi, Alan
Florida Atlantic University, Degree grantor
Roner, Michael R., Thesis advisor
Charles E. Schmidt College of Science
Department of Biological Sciences
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 2000
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, FL
Physical Form: application/pdf
Extent: 50 p.
Language(s): English
Summary: In this study, we report the identification and characterization of a new Reovirus strain isolated from the spinal fluid of a human patient. Unlike other reovirus serotypes that can be isolated from fecal matter, this strain was isolated from the spinal fluid of a patient; which suggests it may be a more pathogenic strain. The new isolate was compared to the three known strains of mammalian reoviruses. We have found that the virus is not a reassortment of the other three serotypes. This was confirmed by examination of the migration rates of the genomic dsRNA segments of all four viruses. The new isolate demonstrated measurable differences in viral protein molecular weights, when compared to the proteins of the three known serotypes. The molecular weight of the sigma I protein of the new isolate, was found to be more then 3Kd smaller then sigma 1 obtained from the other three serotypes. The largest difference in protein molecular weights was found with mu1C, which was about 7Kd smaller. The growth rate of the new virus was very similar to that of the other three serotypes. Some differences were found in select cell lines.
Identifier: 9780599813755 (isbn), 12684 (digitool), FADT12684 (IID), fau:9566 (fedora)
Degree granted: Thesis (M.S.)--Florida Atlantic University, 2000.
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Charles E. Schmidt College of Science
Subject(s): Reoviruses
RNA viruses
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/12684
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.