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Microextraction of total lipid from mesopelagic animals
| Title: | Microextraction of total lipid from mesopelagic animals. |
 76 views 20 downloads |
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| Name(s): |
Reisenbichler, K. R., creator Bailey, T. G., creator Harbor Branch Oceanographic Institute |
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| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 1991 | |
| Publisher: | Elsevier | |
| Extent: | 10 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 3353868 (digitool), FADT3353868 (IID), fau:6654 (fedora), 10.1016/0198-0149(91)90030-J (doi) | |
| Note(s): |
A modification of the Bligh and Dyer lipid extraction method (Canadian Journal of Biochemistry and Physiology, 37, 911–917, 1959) is described. The technique utilizes commercially available Teflon membrane microfiltration units that permit filtration and solvent separation to occur simultaneously. Thus, rapid and efficient microextraction of total lipid (20–200 μg) can be accomplished from tissue homogenates at reduced solvent volumes. The primary advantages of this modification include increased accuracy for small samples, increased productivity and reduced exposure to solvent fumes. The final published version of this manuscript is available at http://www.sciencedirect.com/science/journal/01980149 and may be cited as: Reisenbichler, K. R., & Bailey, T. G. (1991). Microextraction of total lipid from mesopelagic animals. Deep Sea Research Part A. Oceanographic Research Papers, 38(10), 1331-1339. doi:10.1016/0198-0149(91)90030-J Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #821. |
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| Subject(s): |
Lipids--Analysis Lipids--Analysis--Technique |
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| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/3353868 | |
| Links: | http://dx.doi.org/10.1016/0198-0149(91)90030-J | |
| Restrictions on Access: | ©1991 Elsevier Ltd. | |
| Host Institution: | FAU |
