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A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells
| Title: | A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells. |
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| Name(s): |
Longley, Ross E., creator Harmody, Dedra K., creator Harbor Branch Oceanographic Institute |
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| Type of Resource: | text | |
| Genre: | Article | |
| Issuance: | single unit | |
| Date Issued: | 1991 | |
| Publisher: | Nature Publishing Group | |
| Extent: | 11 p. | |
| Physical Description: | ||
| Language(s): | English | |
| Identifier: | 3351944 (digitool), FADT3351944 (IID), fau:6508 (fedora) | |
| Note(s): |
A rapid, colorimetric, microassay for detection of agents which are known agonists/antagonists of protein kinase C (PKC) was developed, utilizing their effects on adherence of EL-4.IL-2 cells. Cells that were incubated with agents which are known inducers of PKC activation, phorbol 12-myristate 13-acetate (PMA), phorbol 12, 13-dibutyrate (PDBu), mezerein and indolactam V, readily adhered to wells of 96 well microtiter plates within 1 - 2 hours, whereas cells incubated with the negative PKC activator, 4α-phorbol 12-myristate 13-acetate (4α-PMA), which is structurally related to PMA (4β-PMA), did not adhere. This manuscript is an author version with the final publication available at http://www.nature.com/ja/index.html and may be cited as: Longley R. E., & Harmody, D. (1991). A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells. The Journal of Antibiotics, 44(1), 93-102. Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #818. |
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| Subject(s): |
Protein kinase C Colorimetric analysis Diglycerides |
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| Persistent Link to This Record: | http://purl.flvc.org/FCLA/DT/3351944 | |
| Restrictions on Access: | ©1991 Nature Publishing Group | |
| Host Institution: | FAU |
