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Associated bacterial communities of two deep-water sponges

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Date Issued:
Title: Associated bacterial communities of two deep-water sponges.
Name(s): Olson, Julie B., creator
McCarthy, Peter J., creator
Type of Resource: text
Genre: Article
Issuance: single unit
Date Issued: 2005
Publisher: Inter-Research Science Center
Extent: 10 p.
Physical Description: pdf
Language(s): English
Identifier: 3350900 (digitool), FADT3350900 (IID), fau:6489 (fedora), 10.3354/ame039047 (doi)
Note(s): A combination of approaches was used to examine the bacterial communities associated with 2 deep-water Scleritoderma spp. sponges. Denaturing gradient gel electrophoresis analysis of partial 16S rRNA genes extracted from sponge tissue was used to determine the structure of the sponge-associated bacterial community. Cultivation studies using a variety of marine-based media and medium additions were performed to characterize the cultivable aerobic heterotrophic bacterial community. DNA was extracted from recovered isolates, mixed, and PCR-denaturing gradient gel electrophoresis was performed to demonstrate the differences between the recoverable microorganisms and the entire bacterial community.
This manuscript is available at and may be cited as: Olson, J. B., & McCarthy, P. J. (2005). Associated bacterial communities of two deep-water sponges. Aquatic Microbial Ecology, 39(1), 47-55. doi:10.3354/ame039047
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1591.
Subject(s): Sponges
Gel electrophoresis
Heterotrophic bacteria
Host-bacteria relationships
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Restrictions on Access: ©2005 Inter-Research Science Center
Owner Institution: FAU