You are here
FAU Collections » FAU Research Repository » Harbor Branch Oceanographic Institute Collection » HBOI Faculty Contributions
- Date Issued:
|Title:||Associated bacterial communities of two deep-water sponges.||
Olson, Julie B., creator
McCarthy, Peter J., creator
|Type of Resource:||text|
|Publisher:||Inter-Research Science Center|
|Identifier:||3350900 (digitool), FADT3350900 (IID), fau:6489 (fedora), 10.3354/ame039047 (doi)|
A combination of approaches was used to examine the bacterial communities associated with 2 deep-water Scleritoderma spp. sponges. Denaturing gradient gel electrophoresis analysis of partial 16S rRNA genes extracted from sponge tissue was used to determine the structure of the sponge-associated bacterial community. Cultivation studies using a variety of marine-based media and medium additions were performed to characterize the cultivable aerobic heterotrophic bacterial community. DNA was extracted from recovered isolates, mixed, and PCR-denaturing gradient gel electrophoresis was performed to demonstrate the differences between the recoverable microorganisms and the entire bacterial community.
This manuscript is available at http://www.int-res.com/ and may be cited as: Olson, J. B., & McCarthy, P. J. (2005). Associated bacterial communities of two deep-water sponges. Aquatic Microbial Ecology, 39(1), 47-55. doi:10.3354/ame039047
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1591.
|Persistent Link to This Record:||http://purl.flvc.org/FCLA/DT/3350900|
|Restrictions on Access:||©2005 Inter-Research Science Center|