You are here

FAU Collections » FAU Research Repository » FAU Theses and Dissertations

Heterologous expression and purification of cell function components -

Download pdf | Full Screen View

Date Issued:
2009
Summary:
Metastatic cancers are problematic because they spread throughout the body. A crucial step in cancer metastasis is the separation of the cancer cells from their surrounding normal cells. This occurs due to suppression or destruction of cell adhesion molecules such as E-cadherin, occludin, and various claudins. The Snail and Slug transcription factors play a direct role in suppressing these cell adhesion molecules through their SNAG repression domain. We explored the possibility of developing an ELISA diagnostics capable of detecting soluble E-cadherin, occludin, and claudin fragments in the serum of cancer patients. Using several bioinformatics tools, unique extracellular antigenic sequences were identified on claudins-1, 4, 16, occludin, and E-cadherin. These sequences were cloned as GST fusion proteins, expressed, and purified in large quantities to raise antibodies. In parallel, expression profiling of metastatic cancer cell lines was carried out to derive a correlation between Snail-Slug expression and suppression of cell adhesion molecules.
Title: Heterologous expression and purification of cell function components -: an effort towards developing an antigen-capture ELISA diagnostics for metastatic cancers.
184 views
112 downloads
Name(s): Irvine, Michael.
Charles E. Schmidt College of Medicine
Department of Biomedical Science
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Issued: 2009
Publisher: Florida Atlantic University
Physical Form: electronic
Extent: x, 70 p. : ill. (some col.)
Language(s): English
Summary: Metastatic cancers are problematic because they spread throughout the body. A crucial step in cancer metastasis is the separation of the cancer cells from their surrounding normal cells. This occurs due to suppression or destruction of cell adhesion molecules such as E-cadherin, occludin, and various claudins. The Snail and Slug transcription factors play a direct role in suppressing these cell adhesion molecules through their SNAG repression domain. We explored the possibility of developing an ELISA diagnostics capable of detecting soluble E-cadherin, occludin, and claudin fragments in the serum of cancer patients. Using several bioinformatics tools, unique extracellular antigenic sequences were identified on claudins-1, 4, 16, occludin, and E-cadherin. These sequences were cloned as GST fusion proteins, expressed, and purified in large quantities to raise antibodies. In parallel, expression profiling of metastatic cancer cell lines was carried out to derive a correlation between Snail-Slug expression and suppression of cell adhesion molecules.
Identifier: 501168612 (oclc), 369191 (digitool), FADT369191 (IID), fau:4262 (fedora)
Note(s): by Michael Irvine.
Thesis (M.S.)--Florida Atlantic University, 2009.
Includes bibliography.
Electronic reproduction. Boca Raton, Fla., 2009. Mode of access: World Wide Web.
Subject(s): Cellular signal transduction
Extracellular matrix proteins
Genetic transcription -- Research
Metalloproteinases -- Inhibitors
Persistent Link to This Record: http://purl.flvc.org/FAU/369191
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU