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Diversity and selection in the major histocompatibility complex

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Date Issued:
2013
Summary:
The Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected geographic patterns of diversity between estuarine and coastal individuals (FST=0.1255, p<0.05), (6) found evidence of positive selection centered in the binding pockets P1, P6 and P9 of the peptide binding region (w=2.08), (7) found that patterns of polymorphism did not closely match patterns of diversity in neutral markers, (8) performed a pilot study with Orcinus orca. The initial findings highlight the need for further comparative work and suggest that silent mutations are not neutral.
Title: Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphins (Tursiops truncatus).
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Name(s): Ferrer, Tatiana.
Charles E. Schmidt College of Science
Department of Biological Sciences
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Issued: 2013
Publisher: Florida Atlantic University
Physical Form: electronic
Extent: x, 75 p. : ill. (some col.)
Language(s): English
Summary: The Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected geographic patterns of diversity between estuarine and coastal individuals (FST=0.1255, p<0.05), (6) found evidence of positive selection centered in the binding pockets P1, P6 and P9 of the peptide binding region (w=2.08), (7) found that patterns of polymorphism did not closely match patterns of diversity in neutral markers, (8) performed a pilot study with Orcinus orca. The initial findings highlight the need for further comparative work and suggest that silent mutations are not neutral.
Identifier: 858866559 (oclc), 3362335 (digitool), FADT3362335 (IID), fau:4173 (fedora)
Note(s): by Tatiana Ferrer.
Thesis (M.S.)--Florida Atlantic University, 2013.
Includes bibliography.
Mode of access: World Wide Web.
System requirements: Adobe Reader.
Subject(s): Major histocompatibility complex
Immunogenetics
Molecular genetics
Endocrine disrupting chemicals
Dolphins -- Atlantic Ocean -- Geographical distribution
Population genetics
Social behavior in animals
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/3362335
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Owner Institution: FAU