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Quantification and Analysis of Cancer-Cell Derived Exosomes
| Title: | Quantification and Analysis of Cancer-Cell Derived Exosomes. |
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| Name(s): |
Urdaneta, Alexandra Iragavarapu-Charyulu, Vijaya Keating, Patricia |
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| Type of Resource: | text | |
| Genre: | Poster | |
| Date Created: | 2018 | |
| Date Issued: | 2018 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Florida | |
| Physical Form: | application/pdf | |
| Extent: | 1 p. | |
| Language(s): | English | |
| Abstract/Description: | Over 90% of cancer-related deaths are due to metastasis. Tumor-cell derived extracellular vesicles or exosomes are thought to contribute to metastasis. However, there is no standardized method to isolate exosomes. We hypothesize that polymer-based kits, in particular ExoQuick-TC (EQTC), may be better for isolating exosomes when compared to ultracentrifugation. In this study, we used three different methods to isolate exosomes from 4T1 murine mammary tumor cells. Samples isolated through ultracentrifugation (UC), EQTC, and Total Exosome Isolation Reagent (TEIR) were analyzed to assess quantity and quality of exosomes by nanoparticle tracking analysis (NTA) and flow cytometry (FC). Using NTA, we found that each method yielded samples with varying average concentration and particle size. FC analysis revealed UC to be the most effective method in yielding a high number of verified exosomes. Standardizing the isolation method and assessment will help in determining the role of exosomes in cancer metastasis. | |
| Identifier: | FAU_SR00000055 (IID) | |
| Collection: | FAU Student Research Digital Collection | |
| Subject(s): | College students --Research --United States. | |
| Held by: | Florida Atlantic University Libraries | |
| Sublocation: | Digital Library | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FAU_SR00000055 | |
| Restrictions on Access: | Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
