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Core clock genes modulate the sleep-feeding axis

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Date Issued:
2014
Summary:
Sleep and feeding are processes essential to nearly all complex organisms, impacting the behavioral output of an animal through homeostatic drive. In Drosophila melanogaster it has been shown that starvation leads to sleep suppression through the signaling of core clock proteins that regulate the animal circadian rhythm. Furthermore overexpression of short neuropeptide, a sleep-promoting inhibitory modulator, increases sleep in animals and alters feeding behavior. While these findings provide a framework of the interaction between the pressures to feed and sleep, they are bi-modal shifts, limiting the understanding of this relationship between to its extreme states. Using the Activity Recording CAFÉ ARC, a tool for measuring the sleep and feeding of individual flies, we tuned either behavior and observed corresponding effects. By shifting food concentrations we are able to control hunger state of an animal while recording its sleep and activity patterns. By coupling this system with a gentle air puff we were also able to control sleep while measuring feeding. We found that the hunger state of an animal had a direct effect on sleep and sleep consolidation. Conversely, we show that increasing sleep pressure led to increased feeding and reduced satiety as calculated through the animal’s prandial behavior. In addition, we show that the direct relationship of the sleep/feeding is disrupted by core clock gene mutations but not by secondary clock genes such as period. By use of the ARC and bi-directional perturbation, we provide a higher resolution understanding of the sleep-feeding axis.
Title: Core clock genes modulate the sleep-feeding axis.
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Name(s): Murphy, Keith Richard
Bruce, Kimberly
Dawson-Scully, Ken
Graduate College
Ja, William W.
Type of Resource: text
Genre: Abstract
Date Created: 2014
Date Issued: 2014
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 1 p.
Language(s): English
Summary: Sleep and feeding are processes essential to nearly all complex organisms, impacting the behavioral output of an animal through homeostatic drive. In Drosophila melanogaster it has been shown that starvation leads to sleep suppression through the signaling of core clock proteins that regulate the animal circadian rhythm. Furthermore overexpression of short neuropeptide, a sleep-promoting inhibitory modulator, increases sleep in animals and alters feeding behavior. While these findings provide a framework of the interaction between the pressures to feed and sleep, they are bi-modal shifts, limiting the understanding of this relationship between to its extreme states. Using the Activity Recording CAFÉ ARC, a tool for measuring the sleep and feeding of individual flies, we tuned either behavior and observed corresponding effects. By shifting food concentrations we are able to control hunger state of an animal while recording its sleep and activity patterns. By coupling this system with a gentle air puff we were also able to control sleep while measuring feeding. We found that the hunger state of an animal had a direct effect on sleep and sleep consolidation. Conversely, we show that increasing sleep pressure led to increased feeding and reduced satiety as calculated through the animal’s prandial behavior. In addition, we show that the direct relationship of the sleep/feeding is disrupted by core clock gene mutations but not by secondary clock genes such as period. By use of the ARC and bi-directional perturbation, we provide a higher resolution understanding of the sleep-feeding axis.
Identifier: FA00005843 (IID)
Collection: FAU Student Research Digital Collection
Note(s): The Fifth Annual Graduate Research Day was organized by Florida Atlantic University’s Graduate Student Association. Graduate students from FAU Colleges present abstracts of original research and posters in a competition for monetary prizes, awards, and recognition
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005843
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.