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Environmental hotspots for antibiotic resistant bacteria arb

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Date Issued:
2014
Abstract/Description:
The overuse and misuse of antibiotics in environmental and clinical settings have been a driving force for the prevalence of bacterial resistance. In constant interaction with these chemicals which can harm them, adaptively and inherently, bacteria have devised resistance mechanisms to combat the deleterious effects posed. In the presence of a particular antibiotic, it is expected there will be selection of resistant micro-organisms and their associated resistance genes if present. In this study, a set of 10 samples were taken from recreational beaches in Ft. Lauderdale, Miami and Hollywood and four different agricultural soils. These soils were enriched after being collected aseptically with three commonly used antibiotics; Ciprofloxacin, Tetracycline and Vancomycin to select for resistant organisms, which produced 29 total samples. A metagenomic analysis was done with 16S rDNA amplification with primers 27F and 1492R which produced 14 out of 29 amplicons producing the expected ~1400 bp fragment from the conserved SSU 16S rDNA region using Agarose gel electrophoresis. From these 14 samples amplified, a second PCR would be run from each enriched antibiotic sample with their respective antibiotic resistance primers eg. vanA-D, tetO and qnrA to identify the resistance genes present expecting that the antibiotic used for enrichment would select for the resistant organisms. Future work includes the sequencing of the amplified resistance genes to help identify novel genetic alterations indicative of new adaptive mechanisms.
Title: Environmental hotspots for antibiotic resistant bacteria arb.
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Name(s): Dawkins, Karim
Esiobu, Nwadiuto
Graduate College
Type of Resource: text
Genre: Abstract
Date Created: 2014
Date Issued: 2014
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 1 p.
Language(s): English
Abstract/Description: The overuse and misuse of antibiotics in environmental and clinical settings have been a driving force for the prevalence of bacterial resistance. In constant interaction with these chemicals which can harm them, adaptively and inherently, bacteria have devised resistance mechanisms to combat the deleterious effects posed. In the presence of a particular antibiotic, it is expected there will be selection of resistant micro-organisms and their associated resistance genes if present. In this study, a set of 10 samples were taken from recreational beaches in Ft. Lauderdale, Miami and Hollywood and four different agricultural soils. These soils were enriched after being collected aseptically with three commonly used antibiotics; Ciprofloxacin, Tetracycline and Vancomycin to select for resistant organisms, which produced 29 total samples. A metagenomic analysis was done with 16S rDNA amplification with primers 27F and 1492R which produced 14 out of 29 amplicons producing the expected ~1400 bp fragment from the conserved SSU 16S rDNA region using Agarose gel electrophoresis. From these 14 samples amplified, a second PCR would be run from each enriched antibiotic sample with their respective antibiotic resistance primers eg. vanA-D, tetO and qnrA to identify the resistance genes present expecting that the antibiotic used for enrichment would select for the resistant organisms. Future work includes the sequencing of the amplified resistance genes to help identify novel genetic alterations indicative of new adaptive mechanisms.
Identifier: FA00005810 (IID)
Collection: FAU Student Research Digital Collection
Note(s): The Fifth Annual Graduate Research Day was organized by Florida Atlantic University’s Graduate Student Association. Graduate students from FAU Colleges present abstracts of original research and posters in a competition for monetary prizes, awards, and recognition
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005810
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.