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DNA Fingerprints and ribotypes of the oral microbiome can distinguish smokers and oral disease patients from healthy non smokers.
| Title: | DNA Fingerprints and ribotypes of the oral microbiome can distinguish smokers and oral disease patients from healthy non smokers. |
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| Name(s): |
Chakraborty, Shreyasee Persaud, Verneshia Vergas, Sonya Graduate College Gautier, Gloryanne Esiobu, Nwadiuto |
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| Type of Resource: | text | |
| Genre: | Abstract | |
| Date Created: | 2014 | |
| Date Issued: | 2014 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Fla. | |
| Physical Form: | application/pdf | |
| Extent: | 1 p. | |
| Language(s): | English | |
| Abstract/Description: | Defining the bacterial communities associated with healthy status will permit rapid detection of shifts associated with disease and foster effective probiotic intervention. This study compares the PCR-RFLP of oral metagenomes and cultured bacterial community, as well as the bacterial diversity profile of smokers, non-smokers and oral disease patients. The goal was to evaluate the stability of bacteria associated with health and capture possible shifts potentially diagnostic of smokers and oral disease. Oral wash samples were obtained from 5 healthy and 5 smokers twice, 3 months apart. Samples from 5 oral disease patients were also collected. Metagenomic and the genomic DNA of a cultured subset were amplified using primer 1492R and 27F.The generated 16SrRNA gene amplicons were used for arestriction digestion assay and bcloning with the Gene Hunter PCRTrap vector and pCR 4- TOPO cloning kits. The restriction fingerprints were statistically tested using Gel Compare II. The cloned 16S genes were sequenced using the ABI GeneAmp 9700 Thermal cycler. Sequences were analyzed by BLAST on the RDB II database and the HOMD. Sau 3AI and Alu I produced the best distinctive markers of 300 bp in healthy nonsmokers and 500bp in smokers in plate wash communities. The 16S sequence data suggest the presence of a core microbiota in all subjects mostly Streptococcus, Gamella, Candidatuse spp and confirmed that the smokers harbored highly diverse and distinct community Neisseria pharynges, Rothia mucilaginosa. Remarkably, there was a high stability of the fingerprints and diversity profile for smokers and nonsmokers after 3 months. | |
| Identifier: | FA00005808 (IID) | |
| Collection: | FAU Student Research Digital Collection | |
| Note(s): | The Fifth Annual Graduate Research Day was organized by Florida Atlantic University’s Graduate Student Association. Graduate students from FAU Colleges present abstracts of original research and posters in a competition for monetary prizes, awards, and recognition | |
| Held by: | Florida Atlantic University Libraries | |
| Sublocation: | Digital Library | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00005808 | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
