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Genetics of Selected Marine Bacteria
- Date Issued:
- 1968
- Summary:
- Marine bacteria are usually described as those bacteria which grow optimally in sea water. Their complex requirements for specific ions (sodium, potassium and magnesium) have been used to differentiate marine from terrigenous bacteria. These requirements, however, vary at the gene level through mutation. Both spontaneous and induced (ultraviolet light) mutations to loss of the ion requirement were observed. Spontaneous frequencies were low (below 10^-6); induced frequencies, high (10^-1 to 10^-8). Back mutations were noticed. A stepwise mechanism was postulated for the mechanism of the mutation involved in the appearance of ion independent bacteria in a population of cells. Genetic analysis of the mechanism by which variations in ion requirements occurred included determination of base ratios and of transformation frequencies. The base ratios of mutant and parent bacteria were similar enough and the transformation frequencies high enough (10^-3 to 10^-5) to show a close relationship between the two types of bacteria. This similarity indicated that, although the genome of marine bacteria, which clearly controls the requirement for specific ions from the values of mutation frequencies, is altered by the action of ultraviolet light, the change was not observable by the methods used. The change could have been induced on the gene level to effect the synthesis of structural or functional proteins (enzymes).
Title: | Genetics of Selected Marine Bacteria. |
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Name(s): |
Pursglove, Diana L. Waddell, Glenn H., Thesis advisor Florida Atlantic University, Degree grantor |
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Type of Resource: | text | |
Genre: | Electronic Thesis Or Dissertation | |
Date Created: | 1968 | |
Date Issued: | 1968 | |
Publisher: | Florida Atlantic University | |
Place of Publication: | Boca Raton, Fla. | |
Physical Form: | application/pdf | |
Extent: | 54 p. | |
Language(s): | English | |
Summary: | Marine bacteria are usually described as those bacteria which grow optimally in sea water. Their complex requirements for specific ions (sodium, potassium and magnesium) have been used to differentiate marine from terrigenous bacteria. These requirements, however, vary at the gene level through mutation. Both spontaneous and induced (ultraviolet light) mutations to loss of the ion requirement were observed. Spontaneous frequencies were low (below 10^-6); induced frequencies, high (10^-1 to 10^-8). Back mutations were noticed. A stepwise mechanism was postulated for the mechanism of the mutation involved in the appearance of ion independent bacteria in a population of cells. Genetic analysis of the mechanism by which variations in ion requirements occurred included determination of base ratios and of transformation frequencies. The base ratios of mutant and parent bacteria were similar enough and the transformation frequencies high enough (10^-3 to 10^-5) to show a close relationship between the two types of bacteria. This similarity indicated that, although the genome of marine bacteria, which clearly controls the requirement for specific ions from the values of mutation frequencies, is altered by the action of ultraviolet light, the change was not observable by the methods used. The change could have been induced on the gene level to effect the synthesis of structural or functional proteins (enzymes). | |
Identifier: | FA00000814 (IID) | |
Degree granted: | Thesis (M.S.)--Florida Atlantic University, 1968. | |
Note(s): | Charles E. Schmidt College of Science | |
Subject(s): |
Marine microbiology Marine bacteria |
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Held by: | Florida Atlantic University Libraries | |
Sublocation: | Digital Library | |
Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00000814 | |
Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
Host Institution: | FAU | |
Is Part of Series: | Florida Atlantic University Digital Library Collections. |