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Comparison of chemotaxonomic methods for the determination of periphyton community composition

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Date Issued:
2010
Summary:
Pigment-based chemotaxonomy uses relative amounts of photosynthetic pigments (biomarkers) within algae samples to determine the algal class composition of each sample. Chemotaxonomy has been applied successfully to phytoplankton communities, but its efficacy for periphyton has not yet been established. This study examined the ability of simultaneous linear equations (SLE), CHEMTAX, and the Bayesian Compositional Estimator (BCE) to determine algal class composition in Florida Everglades periphyton. The methods were applied to artificial datasets, mixed lab cultures of known composition, and Everglades periphyton samples for which microscopic biovolume data was available. All methods were able to return accurate sample compositions for artificial data and mixed lab cultures. Correlation between pigment methods and microscopic results for natural periphyton samples was poor. SLE and CHEMTAX returned similar results for all samples while BCE performed less well.
Title: Comparison of chemotaxonomic methods for the determination of periphyton community composition.
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Name(s): Browne, Jamie L.
Charles E. Schmidt College of Science
Department of Chemistry and Biochemistry
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Issued: 2010
Publisher: Florida Atlantic University
Physical Form: electronic
Extent: ix, 103 p. : ill. (some col.)
Language(s): English
Summary: Pigment-based chemotaxonomy uses relative amounts of photosynthetic pigments (biomarkers) within algae samples to determine the algal class composition of each sample. Chemotaxonomy has been applied successfully to phytoplankton communities, but its efficacy for periphyton has not yet been established. This study examined the ability of simultaneous linear equations (SLE), CHEMTAX, and the Bayesian Compositional Estimator (BCE) to determine algal class composition in Florida Everglades periphyton. The methods were applied to artificial datasets, mixed lab cultures of known composition, and Everglades periphyton samples for which microscopic biovolume data was available. All methods were able to return accurate sample compositions for artificial data and mixed lab cultures. Correlation between pigment methods and microscopic results for natural periphyton samples was poor. SLE and CHEMTAX returned similar results for all samples while BCE performed less well.
Identifier: 624746828 (oclc), 2100582 (digitool), FADT2100582 (IID), fau:3401 (fedora)
Note(s): by Jamie L. Browne.
Thesis (M.S.)--Florida Atlantic University, 2010.
Includes bibliography.
Electronic reproduction. Boca Raton, Fla., 2010. Mode of access: World Wide Web.
Subject(s): Water quality biological assessment -- Florida -- Everglades
Periphyton -- Florida -- Everglades
Water -- Phosphorus content -- Florida -- Everglades
Freshwater algae -- Florida -- Everglades
Persistent Link to This Record: http://purl.flvc.org/FAU/2100582
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU