You are here
Elucidating the role of Semaphorin 7A in breast cancer
| Title: | Elucidating the role of Semaphorin 7A in breast cancer. |
 115 views 27 downloads |
|---|---|---|
| Name(s): |
Garcia-Areas, Ramon A., author lragavarapu-Charyulu, Vijaya, Thesis advisor Florida Atlantic University, Degree grantor Charles E Schmidt College of Science Department of Biomedical Science |
|
| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Date Created: | 2016 | |
| Date Issued: | 2016 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Fla | |
| Physical Form: | application/pdf | |
| Extent: | 96 p | |
| Language(s): | English | |
| Summary: | Solid tumors can hijack many of the same programs used in neurogenesis to enhance tumor growth and metastasis, thereby generating a plethora of neurogenesis-related molecules including semaphorins Among them, we have identified Semaphorin7A (SEMA7A) in breast cancer We first used to the DA-3 mammary tumor model to determine the effect of tumor-derived SEMA7A on immune cells We found that tumor-derived SEMA7A can modulate the production of proangiogenic chemokines CXCL2/MIP-2 and CXCL 1, and prometastatic MMP-9 in macrophages We next aimed to determine the expression and function of SEMA7A in mammary tumor cells We found that SEMA7A is highly expressed in both metastatic human and murine breast cancer cells We show that both TGF-β and hypoxia elicits the production of SEMA 7 A in mammary cells SEMA7 A shRNA silencing in 4T1 cells resulted in decreased mesenchymal markers MMP-3, MMP-13, Vimentin and TGF-β) SEMA7A silenced cells show increased stiffness with reduced migratory and proliferative potential In vivo, SEMA7A silenced 4T1 tumor bearing mice showed decreased tumor growth and metastasis Genetic ablation of host-derived SEMA7A synergized to further decrease the growth and metastasis of 4T1 cells Our findings suggest novel functional roles for SEMA7A in breast cancer and that SEMA7A could be a novel therapeutic target to limit tumor growth and metastasis | |
| Identifier: | FA00004802 (IID) | |
| Degree granted: | Dissertation (PhD)--Florida Atlantic University, 2016 | |
| Collection: | FAU Electronic Theses and Dissertations Collection | |
| Note(s): | Includes bibliography | |
| Subject(s): |
Breast--Cancer--Diagnosis Semaphorins Protein precursors Cellular signal transduction Cell receptors |
|
| Held by: | Florida Atlantic University Libraries | |
| Sublocation: | Digital Library | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00004802 | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
