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Knockdown of KIF9 leads to defects in mitotic entry and progression in mammalian cells

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Date Issued:
2010
Summary:
Kinesin motors bind to microtubules and function in mitosis and intracellular transport depending on the position of the motor domain within the primary sequence (Hirokawa and Noda 2008). KIF9 has recently been shown to be involved in MTOC positioning and mitotic entry in Dictyostelium (Tikhonenko et al. 2009). To determine if a similar role for KIF9 exists in mammalian cells, we are using siRNA-mediated knockdown of KIF9 in COS-7 cells. Analysis of unsynchronized and cell-cycle synchronized cells treated with siRNA to KIF9 reveal that the transition from G2 to M phase is delayed and that mitotic progression is also affected. Additionally, our data indicates that spindle pole function during anaphase may be abnormal in cells treated with siRNA, suggesting a role for KIF9 during that stage.
Title: Knockdown of KIF9 leads to defects in mitotic entry and progression in mammalian cells.
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Name(s): Alsina, Laura.
Harriet L. Wilkes Honors College
Type of Resource: text
Genre: Thesis
Issuance: multipart monograph
Date Issued: 2010
Publisher: Florida Atlantic University
Physical Form: electronic resource
Extent: vii, 25 p. : ill. (some col.)
Language(s): English
Summary: Kinesin motors bind to microtubules and function in mitosis and intracellular transport depending on the position of the motor domain within the primary sequence (Hirokawa and Noda 2008). KIF9 has recently been shown to be involved in MTOC positioning and mitotic entry in Dictyostelium (Tikhonenko et al. 2009). To determine if a similar role for KIF9 exists in mammalian cells, we are using siRNA-mediated knockdown of KIF9 in COS-7 cells. Analysis of unsynchronized and cell-cycle synchronized cells treated with siRNA to KIF9 reveal that the transition from G2 to M phase is delayed and that mitotic progression is also affected. Additionally, our data indicates that spindle pole function during anaphase may be abnormal in cells treated with siRNA, suggesting a role for KIF9 during that stage.
Identifier: 779174977 (oclc), 3334255 (digitool), FADT3334255 (IID), fau:1394 (fedora)
Note(s): by Laura Alsina.
Signature page is unsigned.
Thesis (B.A.)--Florida Atlantic University, Honors College, 2010.
Includes bibliography.
Electronic reproduction. Boca Raton, Fla., 2010. Mode of access: World Wide Web.
Subject(s): Cells -- Motility
Protoplasmic streaming
Cell organelles -- Formation
Cellular signal transduction
Held by: FBoU FAUER
Persistent Link to This Record: http://purl.flvc.org/FAU/3334255
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Host Institution: FAU

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