You are here

FAU Collections » FAU Research Repository » FAU Theses and Dissertations

Colloidal gold labeling of monoclonal antibodies for billfish species identification

Download pdf | Full Screen View

Date Issued:
2000
Summary:
This study established a lateral flow competitive immunoassay with a colloidal gold-monoclonal antibody probe for the qualitative detection of sailfish serum albumin. The test involves mixing a tissue homogenate with a colloidal gold-monoclonal antibody suspension and applying the mixture to a strip of plastic-backed nitrocellulose membrane. The presence of albumin in a target sample competed with adsorbed antigen and prevented the appearance of a pink color on the nitrocellulose membrane. A non-target sample yielded a pink color when gold-labeled monoclonal antibodies bound to sailfish albumin previously absorbed to the nitrocellulose. Three gold particle sizes for antibody conjugation were evaluated and of these, 41 nm was optimal. The optimal pH for conjugation of anti-sailfish antibody to colloidal gold was 7.0. The assay requires only five minutes to perform and utilizes two solutions.
Title: Colloidal gold labeling of monoclonal antibodies for billfish species identification.
86 views
21 downloads
Name(s): Duan, Fei.
Florida Atlantic University, Degree grantor
Hartmann, James X., Thesis advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 2000
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 64 p.
Language(s): English
Summary: This study established a lateral flow competitive immunoassay with a colloidal gold-monoclonal antibody probe for the qualitative detection of sailfish serum albumin. The test involves mixing a tissue homogenate with a colloidal gold-monoclonal antibody suspension and applying the mixture to a strip of plastic-backed nitrocellulose membrane. The presence of albumin in a target sample competed with adsorbed antigen and prevented the appearance of a pink color on the nitrocellulose membrane. A non-target sample yielded a pink color when gold-labeled monoclonal antibodies bound to sailfish albumin previously absorbed to the nitrocellulose. Three gold particle sizes for antibody conjugation were evaluated and of these, 41 nm was optimal. The optimal pH for conjugation of anti-sailfish antibody to colloidal gold was 7.0. The assay requires only five minutes to perform and utilizes two solutions.
Identifier: 9780599747333 (isbn), 15789 (digitool), FADT15789 (IID), fau:12541 (fedora)
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Thesis (M.S.)--Florida Atlantic University, 2000.
Charles E. Schmidt College of Science
Subject(s): Billfishes
Colloidal gold
Immunogold labeling
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/15789
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.