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CREATINE PHOSPHOKINASE ISOENZYME ANALYSIS BY SELECTIVE ACTIVATION WITH THIOLS AND INHIBITION BY ANTIBODIES. AN EVALUATION AND ADAPTATION TO AUTOMATED ANALYSIS

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Date Issued:
1979
Summary:
Analysis of creatine phosphokinase (CPK) isoenzymes is the best clinical test for diagnosis of heart disease. Two new methods for quantitative analysis of these enzymes are selective activation with thiols and specific inhibition by antibodies. These new methods and the conventionally used technique of electrophoresis are comparatively evaluated using assayed controls and cardiac patient serum samples. Results indicate that thiol activation and antibody inhibition are preferred methods for CPK cardiac isoenzyme analysis because they have lower levels of detection, fewer false negative results, and are considerably more efficient than electrophoresis. The new techniques also are adapted to automated spectrophotometric instrumentation, which further contributes to their accuracy and procedural efficiency. Thus, thiol activation and antibody inhibition methods should provide more sensitive and reliable CPK isoenzyme analysis for critical supportive evidence in heart disease diagnosis and treatment
Title: CREATINE PHOSPHOKINASE ISOENZYME ANALYSIS BY SELECTIVE ACTIVATION WITH THIOLS AND INHIBITION BY ANTIBODIES. AN EVALUATION AND ADAPTATION TO AUTOMATED ANALYSIS.
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Name(s): BERNARD, DONALD JAMES.
Florida Atlantic University, Degree grantor
Schultz, Franklin A., Thesis advisor
Charles E. Schmidt College of Science
Department of Chemistry and Biochemistry
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1979
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 101 p.
Language(s): English
Summary: Analysis of creatine phosphokinase (CPK) isoenzymes is the best clinical test for diagnosis of heart disease. Two new methods for quantitative analysis of these enzymes are selective activation with thiols and specific inhibition by antibodies. These new methods and the conventionally used technique of electrophoresis are comparatively evaluated using assayed controls and cardiac patient serum samples. Results indicate that thiol activation and antibody inhibition are preferred methods for CPK cardiac isoenzyme analysis because they have lower levels of detection, fewer false negative results, and are considerably more efficient than electrophoresis. The new techniques also are adapted to automated spectrophotometric instrumentation, which further contributes to their accuracy and procedural efficiency. Thus, thiol activation and antibody inhibition methods should provide more sensitive and reliable CPK isoenzyme analysis for critical supportive evidence in heart disease diagnosis and treatment
Identifier: 13982 (digitool), FADT13982 (IID), fau:10804 (fedora)
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Charles E. Schmidt College of Science
Thesis (M.S.)--Florida Atlantic University, 1979.
Subject(s): Chemistry, Analytic
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/13982
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Owner Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.