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DETACHED LEAF CULTURE OF EICHHORNIA CRASSIPES AND APPLICATION TO THE CULTURE OF ITS PATHOGENS

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Date Issued:
1972
Summary:
Water, Hoagland solution, glucose solutions, kinetin and benzimidazole were tested for their ability to delay senescence of detached Eichhornia crassipes leaves. Water and Hoagland solution failed to sustain chlorophyll past 9 days. Glucose solutions supported contaminating microbial growth and were judged unsatisfactory. Kinetin and benzimidazole solutions maintained a "normal appearance" in detached leaves beyond three weeks culture. Determination of the conversion of protein to amino acids and amines as well as carbohydrate levels in detached leaf culture indicated the optimal culture media were 100 ppm benzimidazole and benzimidazole-kinetin mixture, 50 ppm each. The effective length of culture was three weeks. The utility of this leaf culture system as an alternative to intact host inoculation with pathogenic microorganisms is demonstrated by the isolation and subculture of a pathogenic Alternaria in E. crassipes leaves supported by 50 ppm benzimidazole solution.
Title: DETACHED LEAF CULTURE OF EICHHORNIA CRASSIPES AND APPLICATION TO THE CULTURE OF ITS PATHOGENS.
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Name(s): CAPPLEMAN, LAWRENCE EUGENE, JR.
Florida Atlantic University
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1972
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 63 p.
Language(s): English
Summary: Water, Hoagland solution, glucose solutions, kinetin and benzimidazole were tested for their ability to delay senescence of detached Eichhornia crassipes leaves. Water and Hoagland solution failed to sustain chlorophyll past 9 days. Glucose solutions supported contaminating microbial growth and were judged unsatisfactory. Kinetin and benzimidazole solutions maintained a "normal appearance" in detached leaves beyond three weeks culture. Determination of the conversion of protein to amino acids and amines as well as carbohydrate levels in detached leaf culture indicated the optimal culture media were 100 ppm benzimidazole and benzimidazole-kinetin mixture, 50 ppm each. The effective length of culture was three weeks. The utility of this leaf culture system as an alternative to intact host inoculation with pathogenic microorganisms is demonstrated by the isolation and subculture of a pathogenic Alternaria in E. crassipes leaves supported by 50 ppm benzimidazole solution.
Identifier: 13531 (digitool), FADT13531 (IID), fau:10375 (fedora)
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Thesis (M.S.)--Florida Atlantic University, 1972.
Charles E. Schmidt College of Science
Subject(s): Leaves--Microbiology
Plant diseases
Water hyacinth--Diseases and pests
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/13531
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.